{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/71154"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/71154","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Hapten Binding by Lymphocyte Surface Immunoglobulin and the Subsequent Implications for Affinity Maturation","abstract":"Involvement of surface membrane immunoglobulin (smIg) in the elicitation of affinity maturation has been approached from a number of possible considerations. The salient features are summarized. First, retention time of antigen by membrane associated smIg involves assessment of geometric and probabilistic arguments, in addition to smIg affinity. The functional or observed association constant resulting from multi-determinant-smIg interaction (avidity) may provide a degree of enhancement in antigen binding as to obscure the selective advantage of intrinsic smIg affinity. Second, in the young New Zealand Black animal model, characterized by B lymphocyte hyperactivity (possibly due to T(,s) lymphocyte deficiency), the antibody affinity maturation process occurs at an accelerated rate when compared to another animal model (BALB/cV). Third, in a B cell plasmacytoma (MOPC-315) which had experienced isotype class switch to an (alpha) heavy chain, the immunoglobulin active site was identical between the cell's smIg and secreted immunoglobulin. Protein specificity and affinity for nitrophenyl compounds were determined by quantitative affinity chromatography. Therefore, smIg and secreted immunoglobulin active site identity appear to be a continuous and necessary feature of B cell selection and differentiation. Fourth, naive lymphocytes from BALB/cV mice, selected according to smIg avidity on fluorescein affinity plates failed to demonstrate on anti-fluorescyl response upon adoptive transfer into irradiated recipients. In contrast, selection of antigen-sensitized lymphocytes by affinity panning was effective in removal of certain cell populations. However, the resulting antibody produced from the reconstitution systems were similar in high affinity, regardless of the selected for smIg avidity. Fifth, hapten specific T lymphocytes appeared, within the constraints of the reconstitution system, not to influence antibody affinity maturation and therefore, probably did not influence B cell clonal selection and expansion. Carrier specific T lymphocytes were required for an anti-fluorescyl antibody response.","abstract_html":"Involvement of surface membrane immunoglobulin (smIg) in the elicitation of affinity maturation has been approached from a number of possible considerations. The salient features are summarized. First, retention time of antigen by membrane associated smIg involves assessment of geometric and probabilistic arguments, in addition to smIg affinity. The functional or observed association constant resulting from multi-determinant-smIg interaction (avidity) may provide a degree of enhancement in antigen binding as to obscure the selective advantage of intrinsic smIg affinity. Second, in the young New Zealand Black animal model, characterized by B lymphocyte hyperactivity (possibly due to T(,s) lymphocyte deficiency), the antibody affinity maturation process occurs at an accelerated rate when compared to another animal model (BALB/cV). Third, in a B cell plasmacytoma (MOPC-315) which had experienced isotype class switch to an (alpha) heavy chain, the immunoglobulin active site was identical between the cell's smIg and secreted immunoglobulin. Protein specificity and affinity for nitrophenyl compounds were determined by quantitative affinity chromatography. Therefore, smIg and secreted immunoglobulin active site identity appear to be a continuous and necessary feature of B cell selection and differentiation. Fourth, naive lymphocytes from BALB/cV mice, selected according to smIg avidity on fluorescein affinity plates failed to demonstrate on anti-fluorescyl response upon adoptive transfer into irradiated recipients. In contrast, selection of antigen-sensitized lymphocytes by affinity panning was effective in removal of certain cell populations. However, the resulting antibody produced from the reconstitution systems were similar in high affinity, regardless of the selected for smIg avidity. Fifth, hapten specific T lymphocytes appeared, within the constraints of the reconstitution system, not to influence antibody affinity maturation and therefore, probably did not influence B cell clonal selection and expansion. Carrier specific T lymphocytes were required for an anti-fluorescyl antibody response.","abstract_has_math":false,"creators":["Jarvis, Michael Richard"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-16T06:12:48Z","date_published":"2014-12-16T06:12:48Z","updated_at":"2026-07-22T22:26:04Z","subjects":["Biology, Microbiology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8302896"],"render_values":[{"text":"(UMI)AAI8302896","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/71154","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Jarvis, Michael Richard"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-16T06:12:48Z","10000-01-01","1982"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Microbiology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/71154","(UMI)AAI8302896"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Involvement of surface membrane immunoglobulin (smIg) in the elicitation of affinity maturation has been approached from a number of possible considerations. The salient features are summarized. First, retention time of antigen by membrane associated smIg involves assessment of geometric and probabilistic arguments, in addition to smIg affinity. The functional or observed association constant resulting from multi-determinant-smIg interaction (avidity) may provide a degree of enhancement in antigen binding as to obscure the selective advantage of intrinsic smIg affinity. Second, in the young New Zealand Black animal model, characterized by B lymphocyte hyperactivity (possibly due to T(,s) lymphocyte deficiency), the antibody affinity maturation process occurs at an accelerated rate when compared to another animal model (BALB/cV). Third, in a B cell plasmacytoma (MOPC-315) which had experienced isotype class switch to an (alpha) heavy chain, the immunoglobulin active site was identical between the cell's smIg and secreted immunoglobulin. Protein specificity and affinity for nitrophenyl compounds were determined by quantitative affinity chromatography. Therefore, smIg and secreted immunoglobulin active site identity appear to be a continuous and necessary feature of B cell selection and differentiation. Fourth, naive lymphocytes from BALB/cV mice, selected according to smIg avidity on fluorescein affinity plates failed to demonstrate on anti-fluorescyl response upon adoptive transfer into irradiated recipients. In contrast, selection of antigen-sensitized lymphocytes by affinity panning was effective in removal of certain cell populations. However, the resulting antibody produced from the reconstitution systems were similar in high affinity, regardless of the selected for smIg avidity. Fifth, hapten specific T lymphocytes appeared, within the constraints of the reconstitution system, not to influence antibody affinity maturation and therefore, probably did not influence B cell clonal selection and expansion. Carrier specific T lymphocytes were required for an anti-fluorescyl antibody response.","Made available in DSpace on 2014-12-16T06:12:48Z (GMT). No. of bitstreams: 1 8302896.pdf: 6075024 bytes, checksum: 8e172ce02893b5bf23f40d2547e51fd9 (MD5) Previous issue date: 1982","Embargo set by: Seth Robbins for item 71320 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","193 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1982."]},{"key":"dc:title","label":"Title","values":["Hapten Binding by Lymphocyte Surface Immunoglobulin and the Subsequent Implications for Affinity Maturation"]}]}],"canonical_facts":{"dc:creator":["Jarvis, Michael Richard"],"dc:date":["2014-12-16T06:12:48Z","10000-01-01","1982"],"dc:description":["Involvement of surface membrane immunoglobulin (smIg) in the elicitation of affinity maturation has been approached from a number of possible considerations. The salient features are summarized. First, retention time of antigen by membrane associated smIg involves assessment of geometric and probabilistic arguments, in addition to smIg affinity. The functional or observed association constant resulting from multi-determinant-smIg interaction (avidity) may provide a degree of enhancement in antigen binding as to obscure the selective advantage of intrinsic smIg affinity. Second, in the young New Zealand Black animal model, characterized by B lymphocyte hyperactivity (possibly due to T(,s) lymphocyte deficiency), the antibody affinity maturation process occurs at an accelerated rate when compared to another animal model (BALB/cV). Third, in a B cell plasmacytoma (MOPC-315) which had experienced isotype class switch to an (alpha) heavy chain, the immunoglobulin active site was identical between the cell's smIg and secreted immunoglobulin. Protein specificity and affinity for nitrophenyl compounds were determined by quantitative affinity chromatography. Therefore, smIg and secreted immunoglobulin active site identity appear to be a continuous and necessary feature of B cell selection and differentiation. Fourth, naive lymphocytes from BALB/cV mice, selected according to smIg avidity on fluorescein affinity plates failed to demonstrate on anti-fluorescyl response upon adoptive transfer into irradiated recipients. In contrast, selection of antigen-sensitized lymphocytes by affinity panning was effective in removal of certain cell populations. However, the resulting antibody produced from the reconstitution systems were similar in high affinity, regardless of the selected for smIg avidity. Fifth, hapten specific T lymphocytes appeared, within the constraints of the reconstitution system, not to influence antibody affinity maturation and therefore, probably did not influence B cell clonal selection and expansion. Carrier specific T lymphocytes were required for an anti-fluorescyl antibody response.","Made available in DSpace on 2014-12-16T06:12:48Z (GMT). No. of bitstreams: 1 8302896.pdf: 6075024 bytes, checksum: 8e172ce02893b5bf23f40d2547e51fd9 (MD5) Previous issue date: 1982","Embargo set by: Seth Robbins for item 71320 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","193 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1982."],"dc:identifier":["http://hdl.handle.net/2142/71154","(UMI)AAI8302896"],"dc:subject":["Biology, Microbiology"],"dc:title":["Hapten Binding by Lymphocyte Surface Immunoglobulin and the Subsequent Implications for Affinity Maturation"],"dc:type":["text"],"thesis:degree_discipline":["Microbiology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:04Z"}