{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/70611"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/70611","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Inhibition of Mycelial Growth and Reproduction of Phytophthora Megasperma F. Sp. Glycinea by Trichothecin Produced by Trichothecium Roseum","abstract":"The antagonism between Gliocladium roseum, Trichoderma harzianum, or Trichothecium roseum and Phytophthora megasperma f. sp. glycinea (Pmg) was studied. Culture filtrates of T. roseum inhibited mycelial growth as well as zoosporangenesis of Pmg in culture and soil, but not those of G. roseum and T. harzianum. The antagonism by culture filtrates of T. roseum was found to be due to the production of trichothecin, an antifungal compound.","abstract_html":"The antagonism between Gliocladium roseum, Trichoderma harzianum, or Trichothecium roseum and Phytophthora megasperma f. sp. glycinea (Pmg) was studied. Culture filtrates of T. roseum inhibited mycelial growth as well as zoosporangenesis of Pmg in culture and soil, but not those of G. roseum and T. harzianum. The antagonism by culture filtrates of T. roseum was found to be due to the production of trichothecin, an antifungal compound.","abstract_has_math":false,"creators":["Al-Heeti, Mohammad Badee"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Plant Pathology","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-15T23:52:24Z","date_published":"2014-12-15T23:52:24Z","updated_at":"2026-07-22T22:26:03Z","subjects":["Agriculture","Plant Pathology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8802975"],"render_values":[{"text":"(UMI)AAI8802975","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/70611","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Al-Heeti, Mohammad Badee"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-15T23:52:24Z","1987"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Plant Pathology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Agriculture","Plant Pathology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/70611","(UMI)AAI8802975"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The antagonism between Gliocladium roseum, Trichoderma harzianum, or Trichothecium roseum and Phytophthora megasperma f. sp. glycinea (Pmg) was studied. Culture filtrates of T. roseum inhibited mycelial growth as well as zoosporangenesis of Pmg in culture and soil, but not those of G. roseum and T. harzianum. The antagonism by culture filtrates of T. roseum was found to be due to the production of trichothecin, an antifungal compound.","Trichothecin was identified by using thin-layer chromatography, gas-chromatography (GC), and mass spectrometry. A trichothecin standard completely inhibited mycelial growth, zoosporangenesis, and oospore germination of Pmg in vitro at 6, 1, and 20 $\\mu$g/ml, respectively. Seventeen isolates of T. roseum were compared. Filtrates in which each isolate was grown inhibited both mycelial growth and zoosporangenesis of Pmg in vitro. The GC was used to quantify the amount of trichothecin in the culture filtrate of each isolate. A range of 18-61 mg/L was found among them. Trichothecin production was not related to spore size, colony growth and color, or mycelial dry weight.","T. roseum reduced Pmg inoculum in steam sterilized soil, but only slightly reduced disease on soybean seedlings in the greenhouse. Extraction of soil in which T. roseum was introduced indicated the presence of trichothecin. In the field, T. roseum also reduced the incidence of Pmg in fumigated and nonfumigated soil.","Made available in DSpace on 2014-12-15T23:52:24Z (GMT). No. of bitstreams: 1 8802975.pdf: 4161331 bytes, checksum: 1b020e2a98917e4729fe31f6076765c4 (MD5) Previous issue date: 1987","Embargo set by: Seth Robbins for item 70777 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Open Restriction set for Item 70777 on 2019-07-16T20:41:36Z with date null by mhoh2@illinois.edu.","Open Restriction set for Item 70777 on 2019-07-16T20:41:39Z with date null by mhoh2@illinois.edu.","Open Restriction set for Item 70777 on 2019-07-16T20:41:52Z with date null by mhoh2@illinois.edu.","Open","126 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1987."]},{"key":"dc:title","label":"Title","values":["Inhibition of Mycelial Growth and Reproduction of Phytophthora Megasperma F. Sp. Glycinea by Trichothecin Produced by Trichothecium Roseum"]}]}],"canonical_facts":{"dc:creator":["Al-Heeti, Mohammad Badee"],"dc:date":["2014-12-15T23:52:24Z","1987"],"dc:description":["The antagonism between Gliocladium roseum, Trichoderma harzianum, or Trichothecium roseum and Phytophthora megasperma f. sp. glycinea (Pmg) was studied. Culture filtrates of T. roseum inhibited mycelial growth as well as zoosporangenesis of Pmg in culture and soil, but not those of G. roseum and T. harzianum. The antagonism by culture filtrates of T. roseum was found to be due to the production of trichothecin, an antifungal compound.","Trichothecin was identified by using thin-layer chromatography, gas-chromatography (GC), and mass spectrometry. A trichothecin standard completely inhibited mycelial growth, zoosporangenesis, and oospore germination of Pmg in vitro at 6, 1, and 20 $\\mu$g/ml, respectively. Seventeen isolates of T. roseum were compared. Filtrates in which each isolate was grown inhibited both mycelial growth and zoosporangenesis of Pmg in vitro. The GC was used to quantify the amount of trichothecin in the culture filtrate of each isolate. A range of 18-61 mg/L was found among them. Trichothecin production was not related to spore size, colony growth and color, or mycelial dry weight.","T. roseum reduced Pmg inoculum in steam sterilized soil, but only slightly reduced disease on soybean seedlings in the greenhouse. Extraction of soil in which T. roseum was introduced indicated the presence of trichothecin. In the field, T. roseum also reduced the incidence of Pmg in fumigated and nonfumigated soil.","Made available in DSpace on 2014-12-15T23:52:24Z (GMT). No. of bitstreams: 1 8802975.pdf: 4161331 bytes, checksum: 1b020e2a98917e4729fe31f6076765c4 (MD5) Previous issue date: 1987","Embargo set by: Seth Robbins for item 70777 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Open Restriction set for Item 70777 on 2019-07-16T20:41:36Z with date null by mhoh2@illinois.edu.","Open Restriction set for Item 70777 on 2019-07-16T20:41:39Z with date null by mhoh2@illinois.edu.","Open Restriction set for Item 70777 on 2019-07-16T20:41:52Z with date null by mhoh2@illinois.edu.","Open","126 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1987."],"dc:identifier":["http://hdl.handle.net/2142/70611","(UMI)AAI8802975"],"dc:subject":["Agriculture","Plant Pathology"],"dc:title":["Inhibition of Mycelial Growth and Reproduction of Phytophthora Megasperma F. Sp. Glycinea by Trichothecin Produced by Trichothecium Roseum"],"dc:type":["text"],"thesis:degree_discipline":["Plant Pathology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:03Z"}