The Genome of Bean Golden Mosaic Virus: Restriction Map and Use as a Probe to Sensitively Diagnose Geminivirus Infection
The organization of the genome of bean golden mosaic virus (BGMV) is presented as a model for whitefly-transmitted geminiviruses. The twin-sphere 'geminate' particle is the unit particle and contains one molecule of circular, single-stranded (ss) DNA about 2600 bases long. Linear forms of the DNA, of unimodal size distribution and slightly shorter than the circular form, most likely arise from the circular form by random breakage. The results of restriction analyses and studies of dose/infection relationships in bean protoplasts inoculated with BGMV DNA suggest that the genome is divided between two components encapsidated in separate geminate particles.
University of Illinois - Urbana-Champaign
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University of Illinois at Urbana-Champaign