{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/70565"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/70565","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Studies on the Asparagine-Linked Oligosaccharides From Cartilage-Specific Proteoglycan (Chondrocytes)","abstract":"Chondrocytes synthesize and secrete a cartilage-specific proteoglycan (PG-H) as one of their major products. This proteoglycan has attached to it several types of carbohydrate chains, including chondroitin sulfate, keratan sulfate, O-linked oligosaccharides, and asparagine-linked oligosaccharides. The asparagine-linked oligosaccharides found on PG-H were investigated in these studies.","abstract_html":"Chondrocytes synthesize and secrete a cartilage-specific proteoglycan (PG-H) as one of their major products. This proteoglycan has attached to it several types of carbohydrate chains, including chondroitin sulfate, keratan sulfate, O-linked oligosaccharides, and asparagine-linked oligosaccharides. The asparagine-linked oligosaccharides found on PG-H were investigated in these studies.","abstract_has_math":false,"creators":["Cioffi, Linda Carol"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biochemistry","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-15T23:44:05Z","date_published":"2014-12-15T23:44:05Z","updated_at":"2026-07-22T22:26:03Z","subjects":["Chemistry, Biochemistry"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8721610"],"render_values":[{"text":"(UMI)AAI8721610","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/70565","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Cioffi, Linda Carol"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-15T23:44:05Z","10000-01-01","1987"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemistry, Biochemistry"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/70565","(UMI)AAI8721610"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Chondrocytes synthesize and secrete a cartilage-specific proteoglycan (PG-H) as one of their major products. This proteoglycan has attached to it several types of carbohydrate chains, including chondroitin sulfate, keratan sulfate, O-linked oligosaccharides, and asparagine-linked oligosaccharides. The asparagine-linked oligosaccharides found on PG-H were investigated in these studies.","Methodology was developed for the isolation and separation of standard of standard complex and high mannose type oligosaccharides. This included digesting glycoproteins with N-glycanase and separation of the oligosaccharides according to type by concanavalin-A lectin chromatography. The different oligosaccharide types were then analyzed by high pressure liquid chromatography. This methodology was used in the subsequent studies on the PG-H asparagine-linked oligosaccharides.","Initially, the asparagine-linked oligosaccharides recovered from the culture medium (CM) and cell-associated (Ma) fractions of PG-H from of tibial chondrocytes were labeled with ($\\sp3{\\rm H}$) -mannose and the oligosaccharides were isolated and analyzed. The asparagine-linked oligosaccharides were found to be heterogeneous mixtures of complex and high mannose structures in both the CM and Ma PG-H pools. The ratio of complex to high mannose type oligosaccharides was found to be 6.1 in the CM PG-H and 2.6 in the Ma PG-H. These results suggest the possibility that the CM and Ma PG-H are two separate and distinct molecules, each having its own structure and function.","The chick embryo tibiotarsus was then dissected into zones which differed in their states of differentiation. The PG-H from monolayer cultures of these distinct zones of chondrocytes were isolated and the asparagine-linked oligosaccharides from the CM and Ma pools were characterized are found to be heterogeneous mixtures of oligosaccharide structures. The PG-H from each zone gave its own characteristic array of structures.","In addition to cell culture studies, the asparagine-linked oligosaccharide structures from PG-H in tissue culture were investigated. In every system analyzed--mixed zone cell culture, distinct zone cell culture, mixed zone embryo tibial tissue, and the distinct zones of the chick embryo tibial tissue--the asparagine-linked oligosaccharides were found to be a heterogeneous mixture of complex and high mannose structures.","Made available in DSpace on 2014-12-15T23:44:05Z (GMT). No. of bitstreams: 1 8721610.pdf: 5135947 bytes, checksum: 647390e674d4f90293d9d4e5dccb7e06 (MD5) Previous issue date: 1987","Embargo set by: Seth Robbins for item 70731 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","192 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1987."]},{"key":"dc:title","label":"Title","values":["Studies on the Asparagine-Linked Oligosaccharides From Cartilage-Specific Proteoglycan (Chondrocytes)"]}]}],"canonical_facts":{"dc:creator":["Cioffi, Linda Carol"],"dc:date":["2014-12-15T23:44:05Z","10000-01-01","1987"],"dc:description":["Chondrocytes synthesize and secrete a cartilage-specific proteoglycan (PG-H) as one of their major products. This proteoglycan has attached to it several types of carbohydrate chains, including chondroitin sulfate, keratan sulfate, O-linked oligosaccharides, and asparagine-linked oligosaccharides. The asparagine-linked oligosaccharides found on PG-H were investigated in these studies.","Methodology was developed for the isolation and separation of standard of standard complex and high mannose type oligosaccharides. This included digesting glycoproteins with N-glycanase and separation of the oligosaccharides according to type by concanavalin-A lectin chromatography. The different oligosaccharide types were then analyzed by high pressure liquid chromatography. This methodology was used in the subsequent studies on the PG-H asparagine-linked oligosaccharides.","Initially, the asparagine-linked oligosaccharides recovered from the culture medium (CM) and cell-associated (Ma) fractions of PG-H from of tibial chondrocytes were labeled with ($\\sp3{\\rm H}$) -mannose and the oligosaccharides were isolated and analyzed. The asparagine-linked oligosaccharides were found to be heterogeneous mixtures of complex and high mannose structures in both the CM and Ma PG-H pools. The ratio of complex to high mannose type oligosaccharides was found to be 6.1 in the CM PG-H and 2.6 in the Ma PG-H. These results suggest the possibility that the CM and Ma PG-H are two separate and distinct molecules, each having its own structure and function.","The chick embryo tibiotarsus was then dissected into zones which differed in their states of differentiation. The PG-H from monolayer cultures of these distinct zones of chondrocytes were isolated and the asparagine-linked oligosaccharides from the CM and Ma pools were characterized are found to be heterogeneous mixtures of oligosaccharide structures. The PG-H from each zone gave its own characteristic array of structures.","In addition to cell culture studies, the asparagine-linked oligosaccharide structures from PG-H in tissue culture were investigated. In every system analyzed--mixed zone cell culture, distinct zone cell culture, mixed zone embryo tibial tissue, and the distinct zones of the chick embryo tibial tissue--the asparagine-linked oligosaccharides were found to be a heterogeneous mixture of complex and high mannose structures.","Made available in DSpace on 2014-12-15T23:44:05Z (GMT). No. of bitstreams: 1 8721610.pdf: 5135947 bytes, checksum: 647390e674d4f90293d9d4e5dccb7e06 (MD5) Previous issue date: 1987","Embargo set by: Seth Robbins for item 70731 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","192 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1987."],"dc:identifier":["http://hdl.handle.net/2142/70565","(UMI)AAI8721610"],"dc:subject":["Chemistry, Biochemistry"],"dc:title":["Studies on the Asparagine-Linked Oligosaccharides From Cartilage-Specific Proteoglycan (Chondrocytes)"],"dc:type":["text"],"thesis:degree_discipline":["Biochemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:03Z"}