{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/70527"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/70527","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Eucaryotic Initiation Factor 4b of Wheat Germ Binds to the Translation Initiation Region of Satellite Tobacco Necrosis Virus Ribonucleic Acid (Rna)","abstract":"Highly purified eucaryotic initiation factor 4B from wheat germ binds the intact, monocistronic, uncapped mRNA, STNV RNA, in nitrocellulose filter mediated binding assays with a K(,d) = 1 x 10('-7)M. EIF4B also binds the 5'-terminal 52 nucleotides of STNV {5'- ('32)P}RNA (i.e., the translation initiation site of STNV RNA) with the same efficiency as intact STNV {5'- ('32)P}RNA, but does not bind control samples of randomly selected 5'- ('32)P 52 nucleotide long fragments. EIF4B also binds to 40 nucleotide long fragments, derived from STNV {C- ('125)I}RNA via 40s ribosome protection, as efficiently as it binds to STNV {5'- ('32)P}RNA. Thus, eIF4B binds to an apparently secondary structure free portion of STNV RNA that includes the translation initiation site of STNV RNA. Interestingly, wheat germ ribosomes show the same specificities toward the binding of fragments of STNV RNA. This suggests that eIF4B plays an essential role in the binding of mRNA to ribosomes. In support of this, eIF4B binds to two other eucaryotic mRNAs with similar efficiencies as STNV RNA.","abstract_html":"Highly purified eucaryotic initiation factor 4B from wheat germ binds the intact, monocistronic, uncapped mRNA, STNV RNA, in nitrocellulose filter mediated binding assays with a K(,d) = 1 x 10('-7)M. EIF4B also binds the 5'-terminal 52 nucleotides of STNV {5'- ('32)P}RNA (i.e., the translation initiation site of STNV RNA) with the same efficiency as intact STNV {5'- ('32)P}RNA, but does not bind control samples of randomly selected 5'- ('32)P 52 nucleotide long fragments. EIF4B also binds to 40 nucleotide long fragments, derived from STNV {C- ('125)I}RNA via 40s ribosome protection, as efficiently as it binds to STNV {5'- ('32)P}RNA. Thus, eIF4B binds to an apparently secondary structure free portion of STNV RNA that includes the translation initiation site of STNV RNA. Interestingly, wheat germ ribosomes show the same specificities toward the binding of fragments of STNV RNA. This suggests that eIF4B plays an essential role in the binding of mRNA to ribosomes. In support of this, eIF4B binds to two other eucaryotic mRNAs with similar efficiencies as STNV RNA.","abstract_has_math":false,"creators":["Butler, James Scott"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biochemistry","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-15T23:43:42Z","date_published":"2014-12-15T23:43:42Z","updated_at":"2026-07-22T22:26:03Z","subjects":["Chemistry, Biochemistry"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8409754"],"render_values":[{"text":"(UMI)AAI8409754","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/70527","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Butler, James Scott"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-15T23:43:42Z","10000-01-01","1984"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemistry, Biochemistry"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/70527","(UMI)AAI8409754"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Highly purified eucaryotic initiation factor 4B from wheat germ binds the intact, monocistronic, uncapped mRNA, STNV RNA, in nitrocellulose filter mediated binding assays with a K(,d) = 1 x 10('-7)M. EIF4B also binds the 5'-terminal 52 nucleotides of STNV {5'- ('32)P}RNA (i.e., the translation initiation site of STNV RNA) with the same efficiency as intact STNV {5'- ('32)P}RNA, but does not bind control samples of randomly selected 5'- ('32)P 52 nucleotide long fragments. EIF4B also binds to 40 nucleotide long fragments, derived from STNV {C- ('125)I}RNA via 40s ribosome protection, as efficiently as it binds to STNV {5'- ('32)P}RNA. Thus, eIF4B binds to an apparently secondary structure free portion of STNV RNA that includes the translation initiation site of STNV RNA. Interestingly, wheat germ ribosomes show the same specificities toward the binding of fragments of STNV RNA. This suggests that eIF4B plays an essential role in the binding of mRNA to ribosomes. In support of this, eIF4B binds to two other eucaryotic mRNAs with similar efficiencies as STNV RNA.","Made available in DSpace on 2014-12-15T23:43:42Z (GMT). No. of bitstreams: 1 8409754.pdf: 2567858 bytes, checksum: e51242fc1958b2faec46c0492b086b26 (MD5) Previous issue date: 1984","Embargo set by: Seth Robbins for item 70693 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","102 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1984."]},{"key":"dc:title","label":"Title","values":["Eucaryotic Initiation Factor 4b of Wheat Germ Binds to the Translation Initiation Region of Satellite Tobacco Necrosis Virus Ribonucleic Acid (Rna)"]}]}],"canonical_facts":{"dc:creator":["Butler, James Scott"],"dc:date":["2014-12-15T23:43:42Z","10000-01-01","1984"],"dc:description":["Highly purified eucaryotic initiation factor 4B from wheat germ binds the intact, monocistronic, uncapped mRNA, STNV RNA, in nitrocellulose filter mediated binding assays with a K(,d) = 1 x 10('-7)M. EIF4B also binds the 5'-terminal 52 nucleotides of STNV {5'- ('32)P}RNA (i.e., the translation initiation site of STNV RNA) with the same efficiency as intact STNV {5'- ('32)P}RNA, but does not bind control samples of randomly selected 5'- ('32)P 52 nucleotide long fragments. EIF4B also binds to 40 nucleotide long fragments, derived from STNV {C- ('125)I}RNA via 40s ribosome protection, as efficiently as it binds to STNV {5'- ('32)P}RNA. Thus, eIF4B binds to an apparently secondary structure free portion of STNV RNA that includes the translation initiation site of STNV RNA. Interestingly, wheat germ ribosomes show the same specificities toward the binding of fragments of STNV RNA. This suggests that eIF4B plays an essential role in the binding of mRNA to ribosomes. In support of this, eIF4B binds to two other eucaryotic mRNAs with similar efficiencies as STNV RNA.","Made available in DSpace on 2014-12-15T23:43:42Z (GMT). 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