{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/70505"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/70505","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Estrogen Receptor Regulation of Vitellogenin Gene Expression","abstract":"Stimulation of Xenopus laevis with estradiol-17(beta) induces the massive and prolonged biosynthesis of the egg yolk protein precursor vitellogenin in the liver. Injection of estrogen into male animals previously exposed to estrogen but subsequently withdrawn from the hormone leads to the much earlier appearance of vitellogenin mRNA and a four-fold higher rate of vitellogenin gene transcription in the restimulated animal. The ontogeny of the estrogen receptor indicates that nuclear estrogen receptor is intimately involved with the initiation of vitellogenin gene transcription, but that the maximal rate of gene transcription is independent of the nuclear estrogen receptor concentration. De novo protein synthesis is not requisite for the initiation of gene transcription, providing further evidence that vitellogenesis is a direct response to estradiol. A cytoplasmic estrogen binding protein distinct from the estrogen receptor is characterized. Possible functions of the cytoplasmic binding protein in the vitellogenic response are discussed.","abstract_html":"Stimulation of Xenopus laevis with estradiol-17(beta) induces the massive and prolonged biosynthesis of the egg yolk protein precursor vitellogenin in the liver. Injection of estrogen into male animals previously exposed to estrogen but subsequently withdrawn from the hormone leads to the much earlier appearance of vitellogenin mRNA and a four-fold higher rate of vitellogenin gene transcription in the restimulated animal. The ontogeny of the estrogen receptor indicates that nuclear estrogen receptor is intimately involved with the initiation of vitellogenin gene transcription, but that the maximal rate of gene transcription is independent of the nuclear estrogen receptor concentration. De novo protein synthesis is not requisite for the initiation of gene transcription, providing further evidence that vitellogenesis is a direct response to estradiol. A cytoplasmic estrogen binding protein distinct from the estrogen receptor is characterized. Possible functions of the cytoplasmic binding protein in the vitellogenic response are discussed.","abstract_has_math":false,"creators":["Hayward, Marshall A."],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biochemistry","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-15T23:43:28Z","date_published":"2014-12-15T23:43:28Z","updated_at":"2026-07-22T22:26:03Z","subjects":["Chemistry, Biochemistry"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8209586"],"render_values":[{"text":"(UMI)AAI8209586","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/70505","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Hayward, Marshall A."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-15T23:43:28Z","10000-01-01","1982"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemistry, Biochemistry"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/70505","(UMI)AAI8209586"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Stimulation of Xenopus laevis with estradiol-17(beta) induces the massive and prolonged biosynthesis of the egg yolk protein precursor vitellogenin in the liver. Injection of estrogen into male animals previously exposed to estrogen but subsequently withdrawn from the hormone leads to the much earlier appearance of vitellogenin mRNA and a four-fold higher rate of vitellogenin gene transcription in the restimulated animal. The ontogeny of the estrogen receptor indicates that nuclear estrogen receptor is intimately involved with the initiation of vitellogenin gene transcription, but that the maximal rate of gene transcription is independent of the nuclear estrogen receptor concentration. De novo protein synthesis is not requisite for the initiation of gene transcription, providing further evidence that vitellogenesis is a direct response to estradiol. A cytoplasmic estrogen binding protein distinct from the estrogen receptor is characterized. Possible functions of the cytoplasmic binding protein in the vitellogenic response are discussed.","Made available in DSpace on 2014-12-15T23:43:28Z (GMT). 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Injection of estrogen into male animals previously exposed to estrogen but subsequently withdrawn from the hormone leads to the much earlier appearance of vitellogenin mRNA and a four-fold higher rate of vitellogenin gene transcription in the restimulated animal. The ontogeny of the estrogen receptor indicates that nuclear estrogen receptor is intimately involved with the initiation of vitellogenin gene transcription, but that the maximal rate of gene transcription is independent of the nuclear estrogen receptor concentration. De novo protein synthesis is not requisite for the initiation of gene transcription, providing further evidence that vitellogenesis is a direct response to estradiol. A cytoplasmic estrogen binding protein distinct from the estrogen receptor is characterized. Possible functions of the cytoplasmic binding protein in the vitellogenic response are discussed.","Made available in DSpace on 2014-12-15T23:43:28Z (GMT). No. of bitstreams: 1 8209586.pdf: 2990705 bytes, checksum: 943fd68e8fe3a9e8593932661d290ae3 (MD5) Previous issue date: 1982","Embargo set by: Seth Robbins for item 70671 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","114 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1982."],"dc:identifier":["http://hdl.handle.net/2142/70505","(UMI)AAI8209586"],"dc:subject":["Chemistry, Biochemistry"],"dc:title":["Estrogen Receptor Regulation of Vitellogenin Gene Expression"],"dc:type":["text"],"thesis:degree_discipline":["Biochemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:03Z"}