{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/70403"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/70403","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Covalent Attachment of Coordinatively-Saturated Metal Complexes to Horse Cytochrome-C Lysines","abstract":"The coordinatively saturated complexes $\\rm Ru\\sp{II}(NH\\sb3)\\sb5INH\\sp{2+}$ (INH = isonicotinic acid) and $\\rm Ru\\sp{II}bp\\sb2cmbpH\\sp{2+}$ (bp = 2,2$\\sp\\prime$-bipyridine, cmbp = 4$\\sp\\prime$-methyl-2,2$\\sp\\prime$-bipyridine-4-carboxylic acid) have been covalently attached to horse cytochrome c via a carbodiimide-promoted condensation of the pendant carboxylate of the complexes with the $\\varepsilon$-amine of lysine to form an amide-bond linkage.","abstract_html":"The coordinatively saturated complexes $\\rm Ru\\sp{II}(NH\\sb3)\\sb5INH\\sp{2+}$ (INH = isonicotinic acid) and $\\rm Ru\\sp{II}bp\\sb2cmbpH\\sp{2+}$ (bp = 2,2$\\sp\\prime$-bipyridine, cmbp = 4$\\sp\\prime$-methyl-2,2$\\sp\\prime$-bipyridine-4-carboxylic acid) have been covalently attached to horse cytochrome c via a carbodiimide-promoted condensation of the pendant carboxylate of the complexes with the $\\varepsilon$-amine of lysine to form an amide-bond linkage.","abstract_has_math":true,"creators":["Wallin, Sten Andreas"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Scott, R.A."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-15T23:19:13Z","date_published":"2014-12-15T23:19:13Z","updated_at":"2026-07-22T22:26:02Z","subjects":["Chemistry, Biochemistry","Chemistry, Inorganic"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8815436"],"render_values":[{"text":"(UMI)AAI8815436","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/70403","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Scott, R.A."]},{"key":"dc:creator","label":"Author","values":["Wallin, Sten Andreas"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-15T23:19:13Z","10000-01-01","1988"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemistry, Biochemistry","Chemistry, Inorganic"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/70403","(UMI)AAI8815436"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The coordinatively saturated complexes $\\rm Ru\\sp{II}(NH\\sb3)\\sb5INH\\sp{2+}$ (INH = isonicotinic acid) and $\\rm Ru\\sp{II}bp\\sb2cmbpH\\sp{2+}$ (bp = 2,2$\\sp\\prime$-bipyridine, cmbp = 4$\\sp\\prime$-methyl-2,2$\\sp\\prime$-bipyridine-4-carboxylic acid) have been covalently attached to horse cytochrome c via a carbodiimide-promoted condensation of the pendant carboxylate of the complexes with the $\\varepsilon$-amine of lysine to form an amide-bond linkage.","The reaction of the O-acylisourea (Ru$\\sp{II}$(NH$\\sb3)\\sb5$IN/EDC) $\\sp{3+}$, formed by the reaction of $\\rm Ru\\sp{II}(NH\\sb3)\\sb5IN\\sp{+} (pK\\sb{a}$ = 2.75 $\\pm$ 0.10) with EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide) at pH $\\sim$ 2.7, with cytochrome c at 6.0 $$ 1 s$\\sp{-1}$ for the intramolecular conversion of ${\\rm Ru\\sp{III}}$-${\\rm Fe\\sp{II}}$ to ${\\rm Ru\\sp{III}}$-${\\rm Fe\\sp{III}}$.","Singly-modified Rubp$\\sb2$cmbp/cyt c was prepared from (Rubp$\\sb2$-cmbp/EDC) $\\sp{3+}$ and cytochrome c by the same procedure used for Ru(NH$\\sb3)\\sb5$IN/cyt c. Preliminary peptide mapping results identify the site of modification of some of the components in the mixture of Rubp$\\sb2$cmpb/cyts c. It appears that the product distribution is governed more by hydrophobic interactions between (Rubp$\\sb2$cmbp/-EDC) $\\sp{3+}$ and cytochrome c than by simple electrostatic considerations.","Made available in DSpace on 2014-12-15T23:19:13Z (GMT). No. of bitstreams: 1 8815436.pdf: 4817586 bytes, checksum: 0a3afc8f98b4a60eca00eb5a36b68e13 (MD5) Previous issue date: 1988","Embargo set by: Seth Robbins for item 70569 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","140 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1988."]},{"key":"dc:title","label":"Title","values":["Covalent Attachment of Coordinatively-Saturated Metal Complexes to Horse Cytochrome-C Lysines"]}]}],"canonical_facts":{"dc:contributor":["Scott, R.A."],"dc:creator":["Wallin, Sten Andreas"],"dc:date":["2014-12-15T23:19:13Z","10000-01-01","1988"],"dc:description":["The coordinatively saturated complexes $\\rm Ru\\sp{II}(NH\\sb3)\\sb5INH\\sp{2+}$ (INH = isonicotinic acid) and $\\rm Ru\\sp{II}bp\\sb2cmbpH\\sp{2+}$ (bp = 2,2$\\sp\\prime$-bipyridine, cmbp = 4$\\sp\\prime$-methyl-2,2$\\sp\\prime$-bipyridine-4-carboxylic acid) have been covalently attached to horse cytochrome c via a carbodiimide-promoted condensation of the pendant carboxylate of the complexes with the $\\varepsilon$-amine of lysine to form an amide-bond linkage.","The reaction of the O-acylisourea (Ru$\\sp{II}$(NH$\\sb3)\\sb5$IN/EDC) $\\sp{3+}$, formed by the reaction of $\\rm Ru\\sp{II}(NH\\sb3)\\sb5IN\\sp{+} (pK\\sb{a}$ = 2.75 $\\pm$ 0.10) with EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide) at pH $\\sim$ 2.7, with cytochrome c at 6.0 $$ 1 s$\\sp{-1}$ for the intramolecular conversion of ${\\rm Ru\\sp{III}}$-${\\rm Fe\\sp{II}}$ to ${\\rm Ru\\sp{III}}$-${\\rm Fe\\sp{III}}$.","Singly-modified Rubp$\\sb2$cmbp/cyt c was prepared from (Rubp$\\sb2$-cmbp/EDC) $\\sp{3+}$ and cytochrome c by the same procedure used for Ru(NH$\\sb3)\\sb5$IN/cyt c. Preliminary peptide mapping results identify the site of modification of some of the components in the mixture of Rubp$\\sb2$cmpb/cyts c. It appears that the product distribution is governed more by hydrophobic interactions between (Rubp$\\sb2$cmbp/-EDC) $\\sp{3+}$ and cytochrome c than by simple electrostatic considerations.","Made available in DSpace on 2014-12-15T23:19:13Z (GMT). No. of bitstreams: 1 8815436.pdf: 4817586 bytes, checksum: 0a3afc8f98b4a60eca00eb5a36b68e13 (MD5) Previous issue date: 1988","Embargo set by: Seth Robbins for item 70569 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","140 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1988."],"dc:identifier":["http://hdl.handle.net/2142/70403","(UMI)AAI8815436"],"dc:subject":["Chemistry, Biochemistry","Chemistry, Inorganic"],"dc:title":["Covalent Attachment of Coordinatively-Saturated Metal Complexes to Horse Cytochrome-C Lysines"],"dc:type":["text"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:02Z"}