{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/70358"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/70358","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Molecular Biology of the Cytochrome O Terminal Oxidase of Escherichia Coli (Cloning, Sequencing, Dna)","abstract":"My thesis research has dealt mainly with a genetic approach to studying the cytochrome o terminal oxidase complex of Escherichia coli. Respiratory-deficient mutants of E. coli have been isolated which are unable to grow aerobically on non-fermentable substrates such as succinate and lactate. Spectroscopic and immunological studies showed that these mutants lack the cytochrome o terminal oxidase of the high aeration branch of the respiratory chain. These strains carry mutations in a locus designated cyo which maps at min 10.2 on the E. coli linkage map. The structural genes encoding the cytochrome o oxidase complex have been cloned into the multicopy plasmid pBR322. Strains carrying the cyo plasmids produce 5 to 10 times more cytochrome o oxidase than control strains. The gene products encoded by the cyo plasmids can be immunoprecipitated with monospecific antibodies raised against cytochrome o. The cyo gene locus is located in a 5.5-kb chromosomal DNA segment. The nucleotide sequence of a portion of the cyo gene locus has been determined by Sanger's dideoxynucleotide sequencing method. Further genetic manipulations and a complete DNA sequence analysis of the cyo gene locus will provide valuable information on the protein structure-function relationships.","abstract_html":"My thesis research has dealt mainly with a genetic approach to studying the cytochrome o terminal oxidase complex of Escherichia coli. Respiratory-deficient mutants of E. coli have been isolated which are unable to grow aerobically on non-fermentable substrates such as succinate and lactate. Spectroscopic and immunological studies showed that these mutants lack the cytochrome o terminal oxidase of the high aeration branch of the respiratory chain. These strains carry mutations in a locus designated cyo which maps at min 10.2 on the E. coli linkage map. The structural genes encoding the cytochrome o oxidase complex have been cloned into the multicopy plasmid pBR322. Strains carrying the cyo plasmids produce 5 to 10 times more cytochrome o oxidase than control strains. The gene products encoded by the cyo plasmids can be immunoprecipitated with monospecific antibodies raised against cytochrome o. The cyo gene locus is located in a 5.5-kb chromosomal DNA segment. The nucleotide sequence of a portion of the cyo gene locus has been determined by Sanger's dideoxynucleotide sequencing method. Further genetic manipulations and a complete DNA sequence analysis of the cyo gene locus will provide valuable information on the protein structure-function relationships.","abstract_has_math":false,"creators":["Au, Chi-Tak Doug"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-15T23:18:54Z","date_published":"2014-12-15T23:18:54Z","updated_at":"2026-07-22T22:26:02Z","subjects":["Chemistry, Biochemistry"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8711769"],"render_values":[{"text":"(UMI)AAI8711769","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/70358","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Au, Chi-Tak Doug"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-15T23:18:54Z","10000-01-01","1987"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemistry, Biochemistry"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/70358","(UMI)AAI8711769"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["My thesis research has dealt mainly with a genetic approach to studying the cytochrome o terminal oxidase complex of Escherichia coli. Respiratory-deficient mutants of E. coli have been isolated which are unable to grow aerobically on non-fermentable substrates such as succinate and lactate. Spectroscopic and immunological studies showed that these mutants lack the cytochrome o terminal oxidase of the high aeration branch of the respiratory chain. These strains carry mutations in a locus designated cyo which maps at min 10.2 on the E. coli linkage map. The structural genes encoding the cytochrome o oxidase complex have been cloned into the multicopy plasmid pBR322. Strains carrying the cyo plasmids produce 5 to 10 times more cytochrome o oxidase than control strains. The gene products encoded by the cyo plasmids can be immunoprecipitated with monospecific antibodies raised against cytochrome o. The cyo gene locus is located in a 5.5-kb chromosomal DNA segment. The nucleotide sequence of a portion of the cyo gene locus has been determined by Sanger's dideoxynucleotide sequencing method. Further genetic manipulations and a complete DNA sequence analysis of the cyo gene locus will provide valuable information on the protein structure-function relationships.","Made available in DSpace on 2014-12-15T23:18:54Z (GMT). No. of bitstreams: 1 8711769.pdf: 2580783 bytes, checksum: 456b5835407efc533dfece15adbc7867 (MD5) Previous issue date: 1987","Embargo set by: Seth Robbins for item 70524 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","103 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1987."]},{"key":"dc:title","label":"Title","values":["Molecular Biology of the Cytochrome O Terminal Oxidase of Escherichia Coli (Cloning, Sequencing, Dna)"]}]}],"canonical_facts":{"dc:creator":["Au, Chi-Tak Doug"],"dc:date":["2014-12-15T23:18:54Z","10000-01-01","1987"],"dc:description":["My thesis research has dealt mainly with a genetic approach to studying the cytochrome o terminal oxidase complex of Escherichia coli. Respiratory-deficient mutants of E. coli have been isolated which are unable to grow aerobically on non-fermentable substrates such as succinate and lactate. Spectroscopic and immunological studies showed that these mutants lack the cytochrome o terminal oxidase of the high aeration branch of the respiratory chain. These strains carry mutations in a locus designated cyo which maps at min 10.2 on the E. coli linkage map. The structural genes encoding the cytochrome o oxidase complex have been cloned into the multicopy plasmid pBR322. Strains carrying the cyo plasmids produce 5 to 10 times more cytochrome o oxidase than control strains. The gene products encoded by the cyo plasmids can be immunoprecipitated with monospecific antibodies raised against cytochrome o. The cyo gene locus is located in a 5.5-kb chromosomal DNA segment. The nucleotide sequence of a portion of the cyo gene locus has been determined by Sanger's dideoxynucleotide sequencing method. Further genetic manipulations and a complete DNA sequence analysis of the cyo gene locus will provide valuable information on the protein structure-function relationships.","Made available in DSpace on 2014-12-15T23:18:54Z (GMT). No. of bitstreams: 1 8711769.pdf: 2580783 bytes, checksum: 456b5835407efc533dfece15adbc7867 (MD5) Previous issue date: 1987","Embargo set by: Seth Robbins for item 70524 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","103 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1987."],"dc:identifier":["http://hdl.handle.net/2142/70358","(UMI)AAI8711769"],"dc:subject":["Chemistry, Biochemistry"],"dc:title":["Molecular Biology of the Cytochrome O Terminal Oxidase of Escherichia Coli (Cloning, Sequencing, Dna)"],"dc:type":["text"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:02Z"}