The Determination of Glucuronides in Biological Fluids by Lucigenin Chemiluminescence (beta-Glucuronidase, Glucuronic Acid, Immobilized Enzyme, (Hplc))
The use of certain chemiluminescence reactions as detection techniques for organic compounds has been explored during the past decade. In this research project the chemiluminescence reaction between alkaline lucigenin (10,10'-dimethyl-9,9'-biacridinium nitrate) and glucuronic acid (an important organic reductant in humans) was studied. The main goal of this project was to develop an analytical method for the quantitation of conjugated glucuronic acid (i.e., glucuronides) in physiological fluids using this chemiluminescence reaction as the detection technique.
University of Illinois - Urbana-Champaign
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University of Illinois at Urbana-Champaign