Investigations and Applications of Metal Ion Enhanced Chemiluminescence
Development of an HPLC detector for amino acids based on the suppression of the Co(II) enhanced luminol reaction is presented in this work. Compared to the classical ninhydrin reaction, this method is faster, can be run at room temperature without thermostatting, can determine secondary amino acids without varying the reaction or instrument, has simpler instrumentation, and, in favorable cases, has lower detection limits. The data suggest that the mechanism for inhibition is complexation of Co(II) by the amino acid.
University of Illinois - Urbana-Champaign
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University of Illinois at Urbana-Champaign