{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/70279"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/70279","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Investigations and Applications of Metal Ion Enhanced Chemiluminescence","abstract":"Development of an HPLC detector for amino acids based on the suppression of the Co(II) enhanced luminol reaction is presented in this work. Compared to the classical ninhydrin reaction, this method is faster, can be run at room temperature without thermostatting, can determine secondary amino acids without varying the reaction or instrument, has simpler instrumentation, and, in favorable cases, has lower detection limits. The data suggest that the mechanism for inhibition is complexation of Co(II) by the amino acid.","abstract_html":"Development of an HPLC detector for amino acids based on the suppression of the Co(II) enhanced luminol reaction is presented in this work. Compared to the classical ninhydrin reaction, this method is faster, can be run at room temperature without thermostatting, can determine secondary amino acids without varying the reaction or instrument, has simpler instrumentation, and, in favorable cases, has lower detection limits. The data suggest that the mechanism for inhibition is complexation of Co(II) by the amino acid.","abstract_has_math":false,"creators":["Macdonald, Allan"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-15T23:18:20Z","date_published":"2014-12-15T23:18:20Z","updated_at":"2026-07-22T22:26:02Z","subjects":["Chemistry, Analytical"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8502228"],"render_values":[{"text":"(UMI)AAI8502228","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/70279","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Macdonald, Allan"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-15T23:18:20Z","10000-01-01","1984"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemistry, Analytical"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/70279","(UMI)AAI8502228"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Development of an HPLC detector for amino acids based on the suppression of the Co(II) enhanced luminol reaction is presented in this work. Compared to the classical ninhydrin reaction, this method is faster, can be run at room temperature without thermostatting, can determine secondary amino acids without varying the reaction or instrument, has simpler instrumentation, and, in favorable cases, has lower detection limits. The data suggest that the mechanism for inhibition is complexation of Co(II) by the amino acid.","Also presented in this work are data relating to attempt to obtain selectivity for various metal ions using chemiluminescent detection. The effects of varying reagent concentrations on the chemiluminescent enhancement of various metal ions of the luminol, lophine and gallic acid reactions are shown. These data reveal a complexity which was unexpected and leads to the conclusion that more fundamental work on the role of the metal ion in chemiluminescent reactions is needed before attempts at specificity can be made.","Made available in DSpace on 2014-12-15T23:18:20Z (GMT). No. of bitstreams: 1 8502228.pdf: 4070205 bytes, checksum: b541f8e1be4d81d3f4822e24e5a69242 (MD5) Previous issue date: 1984","Embargo set by: Seth Robbins for item 70445 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","167 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1984."]},{"key":"dc:title","label":"Title","values":["Investigations and Applications of Metal Ion Enhanced Chemiluminescence"]}]}],"canonical_facts":{"dc:creator":["Macdonald, Allan"],"dc:date":["2014-12-15T23:18:20Z","10000-01-01","1984"],"dc:description":["Development of an HPLC detector for amino acids based on the suppression of the Co(II) enhanced luminol reaction is presented in this work. Compared to the classical ninhydrin reaction, this method is faster, can be run at room temperature without thermostatting, can determine secondary amino acids without varying the reaction or instrument, has simpler instrumentation, and, in favorable cases, has lower detection limits. The data suggest that the mechanism for inhibition is complexation of Co(II) by the amino acid.","Also presented in this work are data relating to attempt to obtain selectivity for various metal ions using chemiluminescent detection. The effects of varying reagent concentrations on the chemiluminescent enhancement of various metal ions of the luminol, lophine and gallic acid reactions are shown. These data reveal a complexity which was unexpected and leads to the conclusion that more fundamental work on the role of the metal ion in chemiluminescent reactions is needed before attempts at specificity can be made.","Made available in DSpace on 2014-12-15T23:18:20Z (GMT). No. of bitstreams: 1 8502228.pdf: 4070205 bytes, checksum: b541f8e1be4d81d3f4822e24e5a69242 (MD5) Previous issue date: 1984","Embargo set by: Seth Robbins for item 70445 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","167 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1984."],"dc:identifier":["http://hdl.handle.net/2142/70279","(UMI)AAI8502228"],"dc:subject":["Chemistry, Analytical"],"dc:title":["Investigations and Applications of Metal Ion Enhanced Chemiluminescence"],"dc:type":["text"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:02Z"}