{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/68237"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/68237","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Immunologic Characterization of Soluble Antigen Derived From Cell Culture of Babesia Bovis","abstract":"Soluble cell culture derived antigens of Babesia bovis were studied for their biophysical and chemical properties. The fraction found to be antigenically active by in vitro test and immunogenically potent by inoculation of susceptible animals was thermostable, sensitive to 2-mercaptoethanol, and subject to a partial and a complete destruction by papain and alpha-amlase, respectively. Accordingly, soluble B. bovis antigen appears to be a polysacharide-protein entity or more specifically a relatively heat stable glycoprotein.","abstract_html":"Soluble cell culture derived antigens of Babesia bovis were studied for their biophysical and chemical properties. The fraction found to be antigenically active by in vitro test and immunogenically potent by inoculation of susceptible animals was thermostable, sensitive to 2-mercaptoethanol, and subject to a partial and a complete destruction by papain and alpha-amlase, respectively. Accordingly, soluble B. bovis antigen appears to be a polysacharide-protein entity or more specifically a relatively heat stable glycoprotein.","abstract_has_math":false,"creators":["Osorno, Miguel"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Veterinary Medical Science","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-14T13:28:24Z","date_published":"2014-12-14T13:28:24Z","updated_at":"2026-07-22T22:25:58Z","subjects":["Biology, Veterinary Science"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8114455"],"render_values":[{"text":"(UMI)AAI8114455","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/68237","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Osorno, Miguel"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-14T13:28:24Z","10000-01-01","1981"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Veterinary Medical Science"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Veterinary Science"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/68237","(UMI)AAI8114455"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Soluble cell culture derived antigens of Babesia bovis were studied for their biophysical and chemical properties. The fraction found to be antigenically active by in vitro test and immunogenically potent by inoculation of susceptible animals was thermostable, sensitive to 2-mercaptoethanol, and subject to a partial and a complete destruction by papain and alpha-amlase, respectively. Accordingly, soluble B. bovis antigen appears to be a polysacharide-protein entity or more specifically a relatively heat stable glycoprotein.","Inoculation of cattle with a mixture of soluble B. bovis antigen and saponin adjuvant resulted in a prompt production of blood serum antibodies detectable by an indirect fluorescent antibody (IFA) test. These antibodies persisted at detectable levels for 3 to 4 months. Re-vaccination at 7 months following first inoculation resulted in an anamnestic immune response indicating a recognition and persistence of immunologic memory to primary sensitization.","Thirty 2-year old Hereford cattle which were inoculated with saponin fortified soluble B. bovis antigen, and 20 non-vaccinated similar animals experienced a natural outbreak of babesiosis upon introduction from a tick-free region into a babesiosis endemic area. All animals of the control groups developed signs of the disease and 8 of them died. Among vaccinated animals there was febrile response, hematologic abnormalities and parasitemia, however, all animals remained grossly clinically free of signs and continued to make adequate weight progress.","Made available in DSpace on 2014-12-14T13:28:24Z (GMT). No. of bitstreams: 1 8114455.pdf: 2854768 bytes, checksum: 39849f7989b2eb8e8abcca4546796628 (MD5) Previous issue date: 1981","Embargo set by: Seth Robbins for item 68415 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","82 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1981."]},{"key":"dc:title","label":"Title","values":["Immunologic Characterization of Soluble Antigen Derived From Cell Culture of Babesia Bovis"]}]}],"canonical_facts":{"dc:creator":["Osorno, Miguel"],"dc:date":["2014-12-14T13:28:24Z","10000-01-01","1981"],"dc:description":["Soluble cell culture derived antigens of Babesia bovis were studied for their biophysical and chemical properties. The fraction found to be antigenically active by in vitro test and immunogenically potent by inoculation of susceptible animals was thermostable, sensitive to 2-mercaptoethanol, and subject to a partial and a complete destruction by papain and alpha-amlase, respectively. Accordingly, soluble B. bovis antigen appears to be a polysacharide-protein entity or more specifically a relatively heat stable glycoprotein.","Inoculation of cattle with a mixture of soluble B. bovis antigen and saponin adjuvant resulted in a prompt production of blood serum antibodies detectable by an indirect fluorescent antibody (IFA) test. These antibodies persisted at detectable levels for 3 to 4 months. Re-vaccination at 7 months following first inoculation resulted in an anamnestic immune response indicating a recognition and persistence of immunologic memory to primary sensitization.","Thirty 2-year old Hereford cattle which were inoculated with saponin fortified soluble B. bovis antigen, and 20 non-vaccinated similar animals experienced a natural outbreak of babesiosis upon introduction from a tick-free region into a babesiosis endemic area. All animals of the control groups developed signs of the disease and 8 of them died. Among vaccinated animals there was febrile response, hematologic abnormalities and parasitemia, however, all animals remained grossly clinically free of signs and continued to make adequate weight progress.","Made available in DSpace on 2014-12-14T13:28:24Z (GMT). No. of bitstreams: 1 8114455.pdf: 2854768 bytes, checksum: 39849f7989b2eb8e8abcca4546796628 (MD5) Previous issue date: 1981","Embargo set by: Seth Robbins for item 68415 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","82 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1981."],"dc:identifier":["http://hdl.handle.net/2142/68237","(UMI)AAI8114455"],"dc:language":["eng"],"dc:subject":["Biology, Veterinary Science"],"dc:title":["Immunologic Characterization of Soluble Antigen Derived From Cell Culture of Babesia Bovis"],"dc:type":["text"],"thesis:degree_discipline":["Veterinary Medical Science"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:58Z"}