Cellular Immunity in Hamsters After Immunization With Vaccinia Virus: Studies on Macrophage Functional Heterogeneity and Interactions Between Natural Killer Cells and Macrophages
This study demonstrated that macrophage (M(phi)) subpopulations can be isolated from normal and vaccinia virus-immune peritoneal exudate cells after separation on bovine serum albumin (BSA) discontinuous density gradients. The M(phi) subpopulations from normal and early- or late-immune animals were all phagocytic but differed in their ability to mediate cytotoxicity. Normal M(phi) subpopulations were not cytotoxic, whereas the 40/30 and pellet fractions collected from BSA gradients of immune M(phi)s were cytotoxic. In contrast, M(phi)s isolated from the lighter fractions were found to be poor killer cells.
University of Illinois - Urbana-Champaign
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University of Illinois at Urbana-Champaign