Cellular Immunity in Hamsters After Immunization With Vaccinia Virus: Studies on Macrophage Functional Heterogeneity and Interactions Between Natural Killer Cells and Macrophages
type: CreativeWork
description: This study demonstrated that macrophage (M(phi)) subpopulations can be isolated from normal and vaccinia virus-immune peritoneal exudate cells after separation on bovine serum albumin (BSA) discontinuous density gradients. The M(phi) subpopulations from normal and early- or late-immune animals were all phagocytic but differed in their ability to mediate cytotoxicity. Normal M(phi) subpopulations were not cytotoxic, whereas the 40/30 and pellet fractions collected from BSA gradients of immune M(phi)s were cytotoxic. In contrast, M(phi)s isolated from the lighter fractions were found to be poor killer cells.
abstract: This study demonstrated that macrophage (M(phi)) subpopulations can be isolated from normal and vaccinia virus-immune peritoneal exudate cells after separation on bovine serum albumin (BSA) discontinuous density gradients. The M(phi) subpopulations from normal and early- or late-immune animals were all phagocytic but differed in their ability to mediate cytotoxicity. Normal M(phi) subpopulations were not cytotoxic, whereas the 40/30 and pellet fractions collected from BSA gradients of immune M(phi)s were cytotoxic. In contrast, M(phi)s isolated from the lighter fractions were found to be poor killer cells.
author: Chapes, Stephen Keith
language: eng
keywords: Health Sciences, Immunology
date published: 2014-12-14T13:28:23Z
date modified: 2026-07-22T22:25:58Z
provider: University of Illinois - Urbana-Champaign
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Illinois at Urbana-Champaign

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2142/68235