{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/67450"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/67450","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"The Histology and Histochemistry of The Wound-Healing Process in Geranium Cuttings in Relationship to Basal Stem Rot Caused by Pythium Ultimum","abstract":"Geraniums are largely propagated by cuttings even though new seed strains are now becoming popular. They are available as unrooted, callused redi-root (adventitious root initials just beginning to emerge) or fully rooted cuttings. Blackleg, caused by Pythium species, is a problem in geranium propagation. Cuttings of Pelargonium hortorum 'Yours Truly' were inoculated with an isolate of Pythium ultimum. Extensive basal stem rot occurred on all freshly broken cuttings. A restricted basal stem rot occurred on cuttings that had healed 1 day in soil prior to inoculation. Basal stem rot rarely occurred on cuttings that had healed 2 days or longer prior to inoculation. The wound-healing process in cuttings was studied using histological and histochemical techniques. After 1 day a distinct morphological change occurred in the cell walls of all tissues nearest the wounded surface. The cell walls became thickened due to deposition of substance in a layer around the cell wall. Histochemical tests determined this substance to be suberin. Suberin deposits were also observed in the intercellular spaces. A wound callus developed after 7-10 days. A suberized wound periderm developed after 14-21 days. Disease severity and the length of time after wound healing began were inversely correlated.","abstract_html":"Geraniums are largely propagated by cuttings even though new seed strains are now becoming popular. They are available as unrooted, callused redi-root (adventitious root initials just beginning to emerge) or fully rooted cuttings. Blackleg, caused by Pythium species, is a problem in geranium propagation. Cuttings of Pelargonium hortorum 'Yours Truly' were inoculated with an isolate of Pythium ultimum. Extensive basal stem rot occurred on all freshly broken cuttings. A restricted basal stem rot occurred on cuttings that had healed 1 day in soil prior to inoculation. Basal stem rot rarely occurred on cuttings that had healed 2 days or longer prior to inoculation. The wound-healing process in cuttings was studied using histological and histochemical techniques. After 1 day a distinct morphological change occurred in the cell walls of all tissues nearest the wounded surface. The cell walls became thickened due to deposition of substance in a layer around the cell wall. Histochemical tests determined this substance to be suberin. Suberin deposits were also observed in the intercellular spaces. A wound callus developed after 7-10 days. A suberized wound periderm developed after 14-21 days. Disease severity and the length of time after wound healing began were inversely correlated.","abstract_has_math":false,"creators":["Cline, Molly Niedbalski"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Plant Pathology","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-14T04:37:48Z","date_published":"2014-12-14T04:37:48Z","updated_at":"2026-07-22T22:25:57Z","subjects":["Agriculture, Plant Pathology"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8108470"],"render_values":[{"text":"(UMI)AAI8108470","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/67450","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Cline, Molly Niedbalski"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-14T04:37:48Z","10000-01-01","1980"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Plant Pathology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Agriculture, Plant Pathology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/67450","(UMI)AAI8108470"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Geraniums are largely propagated by cuttings even though new seed strains are now becoming popular. They are available as unrooted, callused redi-root (adventitious root initials just beginning to emerge) or fully rooted cuttings. Blackleg, caused by Pythium species, is a problem in geranium propagation. Cuttings of Pelargonium hortorum 'Yours Truly' were inoculated with an isolate of Pythium ultimum. Extensive basal stem rot occurred on all freshly broken cuttings. A restricted basal stem rot occurred on cuttings that had healed 1 day in soil prior to inoculation. Basal stem rot rarely occurred on cuttings that had healed 2 days or longer prior to inoculation. The wound-healing process in cuttings was studied using histological and histochemical techniques. After 1 day a distinct morphological change occurred in the cell walls of all tissues nearest the wounded surface. The cell walls became thickened due to deposition of substance in a layer around the cell wall. Histochemical tests determined this substance to be suberin. Suberin deposits were also observed in the intercellular spaces. A wound callus developed after 7-10 days. A suberized wound periderm developed after 14-21 days. Disease severity and the length of time after wound healing began were inversely correlated.","Made available in DSpace on 2014-12-14T04:37:48Z (GMT). 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They are available as unrooted, callused redi-root (adventitious root initials just beginning to emerge) or fully rooted cuttings. Blackleg, caused by Pythium species, is a problem in geranium propagation. Cuttings of Pelargonium hortorum 'Yours Truly' were inoculated with an isolate of Pythium ultimum. Extensive basal stem rot occurred on all freshly broken cuttings. A restricted basal stem rot occurred on cuttings that had healed 1 day in soil prior to inoculation. Basal stem rot rarely occurred on cuttings that had healed 2 days or longer prior to inoculation. The wound-healing process in cuttings was studied using histological and histochemical techniques. After 1 day a distinct morphological change occurred in the cell walls of all tissues nearest the wounded surface. The cell walls became thickened due to deposition of substance in a layer around the cell wall. Histochemical tests determined this substance to be suberin. Suberin deposits were also observed in the intercellular spaces. A wound callus developed after 7-10 days. A suberized wound periderm developed after 14-21 days. Disease severity and the length of time after wound healing began were inversely correlated.","Made available in DSpace on 2014-12-14T04:37:48Z (GMT). No. of bitstreams: 1 8108470.pdf: 5740724 bytes, checksum: dc9c39e09c13bfb02696dcffdc62258c (MD5) Previous issue date: 1980","Embargo set by: Seth Robbins for item 67628 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","127 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1980."],"dc:identifier":["http://hdl.handle.net/2142/67450","(UMI)AAI8108470"],"dc:language":["eng"],"dc:subject":["Agriculture, Plant Pathology"],"dc:title":["The Histology and Histochemistry of The Wound-Healing Process in Geranium Cuttings in Relationship to Basal Stem Rot Caused by Pythium Ultimum"],"dc:type":["text"],"thesis:degree_discipline":["Plant Pathology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:57Z"}