{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/67414"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/67414","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Characterization of Tumorigenic Proteins","abstract":"The 17K, 84K, and 94K SV40 T antigens from SV40 permissive and transformed cells were analyzed by tryptic peptide mapping on ion exchange columns. The tryptic peptides were mapped relative to the SV40 DNA and to their known functions. The aminotermini of the 17K, 84K and 94K T antigens are identical. The 84K protein probably arises from the 94K T antigen by proteolytic cleavage of the 94K T antigen carboxyterminus. The 48K and 55K nonviral antigens, proteins immunoprecipitable by SV40 T antibody from SV40 permissive and transformed cells, were compared to the SV40 large T antigen and to each other by tryptic peptide mapping. The 48K and 55K nonviral antigens are not degradation products of the SV40 T antigens and at most are only slightly related to the SV40 T antigens. The 48K and 55K nonviral antigens are slightly related to each other, sharing about 3 methionine containing tryptic peptides. Four SV40 transformed human cell lines, one SV40 transformed mouse cell line, and eight SV40 transformed human/mouse somatic cell hybrid cell lines were examined for the expression of nonviral antigens. Differences were found in the patterns of expression of the nonviral antigens among the human cell lines, between the human and the mouse cell lines, and between the human/mouse somatic cell hybrid cell lines and the human lines from which they were derived. Two of the human cell lines expressed only a 55K nonviral antigen; two expressed both 55K nonviral antigen; two expressed both 55K and 52K nonviral antigens. The mouse cell line expressed both a 55K and a 48K nonviral antigen, except for one. The Balb IV-8 cell line expressed 55K and 52K nonviral antigens. By comparing the tryptic peptides fractionated on cation exchange columns of the nonviral antigens it was determined that: (1) The 48K nonviral antigen in human/mouse hybrids is encoded by a mouse chromosome. (2) The 52K nonviral antigen in the Balb IV-8 human/mouse hybrid is encoded by a mouse chromosome and is probably a precursor to the mouse 48K nonviral antigen. (3) The 55K nonviral antigen in the C57-VII-6 human/mouse hybrid is encoded by a human chromosome; while in the Balb IV-8 human/mouse hybrid the 55K protein band is encoded by a human chromosome and probably by a mouse chromosome also. (4) If the gene encoding the human nonviral antigen is located on a single chromosome, it is located on human chromosome 7.","abstract_html":"The 17K, 84K, and 94K SV40 T antigens from SV40 permissive and transformed cells were analyzed by tryptic peptide mapping on ion exchange columns. The tryptic peptides were mapped relative to the SV40 DNA and to their known functions. The aminotermini of the 17K, 84K and 94K T antigens are identical. The 84K protein probably arises from the 94K T antigen by proteolytic cleavage of the 94K T antigen carboxyterminus. The 48K and 55K nonviral antigens, proteins immunoprecipitable by SV40 T antibody from SV40 permissive and transformed cells, were compared to the SV40 large T antigen and to each other by tryptic peptide mapping. The 48K and 55K nonviral antigens are not degradation products of the SV40 T antigens and at most are only slightly related to the SV40 T antigens. The 48K and 55K nonviral antigens are slightly related to each other, sharing about 3 methionine containing tryptic peptides. Four SV40 transformed human cell lines, one SV40 transformed mouse cell line, and eight SV40 transformed human/mouse somatic cell hybrid cell lines were examined for the expression of nonviral antigens. Differences were found in the patterns of expression of the nonviral antigens among the human cell lines, between the human and the mouse cell lines, and between the human/mouse somatic cell hybrid cell lines and the human lines from which they were derived. Two of the human cell lines expressed only a 55K nonviral antigen; two expressed both 55K nonviral antigen; two expressed both 55K and 52K nonviral antigens. The mouse cell line expressed both a 55K and a 48K nonviral antigen, except for one. The Balb IV-8 cell line expressed 55K and 52K nonviral antigens. By comparing the tryptic peptides fractionated on cation exchange columns of the nonviral antigens it was determined that: (1) The 48K nonviral antigen in human/mouse hybrids is encoded by a mouse chromosome. (2) The 52K nonviral antigen in the Balb IV-8 human/mouse hybrid is encoded by a mouse chromosome and is probably a precursor to the mouse 48K nonviral antigen. (3) The 55K nonviral antigen in the C57-VII-6 human/mouse hybrid is encoded by a human chromosome; while in the Balb IV-8 human/mouse hybrid the 55K protein band is encoded by a human chromosome and probably by a mouse chromosome also. (4) If the gene encoding the human nonviral antigen is located on a single chromosome, it is located on human chromosome 7.","abstract_has_math":false,"creators":["Stitt, David Thomas"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biochemistry","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-14T04:27:58Z","date_published":"2014-12-14T04:27:58Z","updated_at":"2026-07-22T22:25:57Z","subjects":["Chemistry, Biochemistry"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8127706"],"render_values":[{"text":"(UMI)AAI8127706","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/67414","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Stitt, David Thomas"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-14T04:27:58Z","10000-01-01","1981"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemistry, Biochemistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/67414","(UMI)AAI8127706"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The 17K, 84K, and 94K SV40 T antigens from SV40 permissive and transformed cells were analyzed by tryptic peptide mapping on ion exchange columns. The tryptic peptides were mapped relative to the SV40 DNA and to their known functions. The aminotermini of the 17K, 84K and 94K T antigens are identical. The 84K protein probably arises from the 94K T antigen by proteolytic cleavage of the 94K T antigen carboxyterminus. The 48K and 55K nonviral antigens, proteins immunoprecipitable by SV40 T antibody from SV40 permissive and transformed cells, were compared to the SV40 large T antigen and to each other by tryptic peptide mapping. The 48K and 55K nonviral antigens are not degradation products of the SV40 T antigens and at most are only slightly related to the SV40 T antigens. The 48K and 55K nonviral antigens are slightly related to each other, sharing about 3 methionine containing tryptic peptides. Four SV40 transformed human cell lines, one SV40 transformed mouse cell line, and eight SV40 transformed human/mouse somatic cell hybrid cell lines were examined for the expression of nonviral antigens. Differences were found in the patterns of expression of the nonviral antigens among the human cell lines, between the human and the mouse cell lines, and between the human/mouse somatic cell hybrid cell lines and the human lines from which they were derived. Two of the human cell lines expressed only a 55K nonviral antigen; two expressed both 55K nonviral antigen; two expressed both 55K and 52K nonviral antigens. The mouse cell line expressed both a 55K and a 48K nonviral antigen, except for one. The Balb IV-8 cell line expressed 55K and 52K nonviral antigens. By comparing the tryptic peptides fractionated on cation exchange columns of the nonviral antigens it was determined that: (1) The 48K nonviral antigen in human/mouse hybrids is encoded by a mouse chromosome. (2) The 52K nonviral antigen in the Balb IV-8 human/mouse hybrid is encoded by a mouse chromosome and is probably a precursor to the mouse 48K nonviral antigen. (3) The 55K nonviral antigen in the C57-VII-6 human/mouse hybrid is encoded by a human chromosome; while in the Balb IV-8 human/mouse hybrid the 55K protein band is encoded by a human chromosome and probably by a mouse chromosome also. (4) If the gene encoding the human nonviral antigen is located on a single chromosome, it is located on human chromosome 7.","Made available in DSpace on 2014-12-14T04:27:58Z (GMT). No. of bitstreams: 1 8127706.pdf: 5009072 bytes, checksum: e92cddc8fde884c4d82ea66a777c349a (MD5) Previous issue date: 1981","Embargo set by: Seth Robbins for item 67592 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","173 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1981."]},{"key":"dc:title","label":"Title","values":["Characterization of Tumorigenic Proteins"]}]}],"canonical_facts":{"dc:creator":["Stitt, David Thomas"],"dc:date":["2014-12-14T04:27:58Z","10000-01-01","1981"],"dc:description":["The 17K, 84K, and 94K SV40 T antigens from SV40 permissive and transformed cells were analyzed by tryptic peptide mapping on ion exchange columns. The tryptic peptides were mapped relative to the SV40 DNA and to their known functions. The aminotermini of the 17K, 84K and 94K T antigens are identical. The 84K protein probably arises from the 94K T antigen by proteolytic cleavage of the 94K T antigen carboxyterminus. The 48K and 55K nonviral antigens, proteins immunoprecipitable by SV40 T antibody from SV40 permissive and transformed cells, were compared to the SV40 large T antigen and to each other by tryptic peptide mapping. The 48K and 55K nonviral antigens are not degradation products of the SV40 T antigens and at most are only slightly related to the SV40 T antigens. The 48K and 55K nonviral antigens are slightly related to each other, sharing about 3 methionine containing tryptic peptides. Four SV40 transformed human cell lines, one SV40 transformed mouse cell line, and eight SV40 transformed human/mouse somatic cell hybrid cell lines were examined for the expression of nonviral antigens. Differences were found in the patterns of expression of the nonviral antigens among the human cell lines, between the human and the mouse cell lines, and between the human/mouse somatic cell hybrid cell lines and the human lines from which they were derived. Two of the human cell lines expressed only a 55K nonviral antigen; two expressed both 55K nonviral antigen; two expressed both 55K and 52K nonviral antigens. The mouse cell line expressed both a 55K and a 48K nonviral antigen, except for one. The Balb IV-8 cell line expressed 55K and 52K nonviral antigens. By comparing the tryptic peptides fractionated on cation exchange columns of the nonviral antigens it was determined that: (1) The 48K nonviral antigen in human/mouse hybrids is encoded by a mouse chromosome. (2) The 52K nonviral antigen in the Balb IV-8 human/mouse hybrid is encoded by a mouse chromosome and is probably a precursor to the mouse 48K nonviral antigen. (3) The 55K nonviral antigen in the C57-VII-6 human/mouse hybrid is encoded by a human chromosome; while in the Balb IV-8 human/mouse hybrid the 55K protein band is encoded by a human chromosome and probably by a mouse chromosome also. (4) If the gene encoding the human nonviral antigen is located on a single chromosome, it is located on human chromosome 7.","Made available in DSpace on 2014-12-14T04:27:58Z (GMT). No. of bitstreams: 1 8127706.pdf: 5009072 bytes, checksum: e92cddc8fde884c4d82ea66a777c349a (MD5) Previous issue date: 1981","Embargo set by: Seth Robbins for item 67592 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","173 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1981."],"dc:identifier":["http://hdl.handle.net/2142/67414","(UMI)AAI8127706"],"dc:language":["eng"],"dc:subject":["Chemistry, Biochemistry"],"dc:title":["Characterization of Tumorigenic Proteins"],"dc:type":["text"],"thesis:degree_discipline":["Biochemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:57Z"}