{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/67234"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/67234","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Investigation of the Chemiluminescent Reaction Between Lucigenin and Organic Reductants and Its Application as a Detector for High Pressure Liquid Chromatography","abstract":"The reaction of basic lucigenin (N,N'-dimethyl-9,9'-biacridinium dinitrate) with organic reductants was investigated as an analytical tool. Investigation of this reaction was carried out in three distinct steps: (1) stopped flow investigation of the reaction parameters; (2) development of a flow injection system for investigation, specifically the development of a chemiluminescence detector for HPLC, and; (3) application of the chemiluminescence detector to the determination of ascorbic acid and dehydroascorbic acid.","abstract_html":"The reaction of basic lucigenin (N,N'-dimethyl-9,9'-biacridinium dinitrate) with organic reductants was investigated as an analytical tool. Investigation of this reaction was carried out in three distinct steps: (1) stopped flow investigation of the reaction parameters; (2) development of a flow injection system for investigation, specifically the development of a chemiluminescence detector for HPLC, and; (3) application of the chemiluminescence detector to the determination of ascorbic acid and dehydroascorbic acid.","abstract_has_math":false,"creators":["Veazey, Robert Lawrence"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-13T20:10:58Z","date_published":"2014-12-13T20:10:58Z","updated_at":"2026-07-22T22:25:57Z","subjects":["Chemistry, Analytical"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8026608"],"render_values":[{"text":"(UMI)AAI8026608","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/67234","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Veazey, Robert Lawrence"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-13T20:10:58Z","10000-01-01","1980"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemistry, Analytical"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/67234","(UMI)AAI8026608"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The reaction of basic lucigenin (N,N'-dimethyl-9,9'-biacridinium dinitrate) with organic reductants was investigated as an analytical tool. Investigation of this reaction was carried out in three distinct steps: (1) stopped flow investigation of the reaction parameters; (2) development of a flow injection system for investigation, specifically the development of a chemiluminescence detector for HPLC, and; (3) application of the chemiluminescence detector to the determination of ascorbic acid and dehydroascorbic acid.","The stopped flow data indicated that simple one-component samples of ascorbic acid, creatinine, uric acid, glutathione, glucuronic acid, lactose, and glucose can be quantitatively measured within their important clinical concentration ranges. Two component mixtures of glucose and ascorbic acid were observed to exhibit a response which was a simple addition of their individual responses.","Continuous flow instrumentation was designed, built, and tested. Reagent delivery and flow cell design were shown to play an important role in achieving reproducible and reliable data.","Ascorbic acid and dehydroascorbic acid were quantitated in the effluent of an HPLC, utilizing the chemiluminescence detector. Vitamin C tablets were assayed using the technique, yielding data that agreed to within 1-5% of titration results. A separation of glucose, glucuronic acid, uric acid, and ascorbic acid on an anion exchange column, at concentrations within their clinical ranges, was detected via the chemiluminescence method.","Made available in DSpace on 2014-12-13T20:10:58Z (GMT). No. of bitstreams: 1 8026608.pdf: 5944238 bytes, checksum: 189e2acddae7c8340ad836c7da221c0b (MD5) Previous issue date: 1980","Embargo set by: Seth Robbins for item 67412 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","212 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1980."]},{"key":"dc:title","label":"Title","values":["Investigation of the Chemiluminescent Reaction Between Lucigenin and Organic Reductants and Its Application as a Detector for High Pressure Liquid Chromatography"]}]}],"canonical_facts":{"dc:creator":["Veazey, Robert Lawrence"],"dc:date":["2014-12-13T20:10:58Z","10000-01-01","1980"],"dc:description":["The reaction of basic lucigenin (N,N'-dimethyl-9,9'-biacridinium dinitrate) with organic reductants was investigated as an analytical tool. Investigation of this reaction was carried out in three distinct steps: (1) stopped flow investigation of the reaction parameters; (2) development of a flow injection system for investigation, specifically the development of a chemiluminescence detector for HPLC, and; (3) application of the chemiluminescence detector to the determination of ascorbic acid and dehydroascorbic acid.","The stopped flow data indicated that simple one-component samples of ascorbic acid, creatinine, uric acid, glutathione, glucuronic acid, lactose, and glucose can be quantitatively measured within their important clinical concentration ranges. Two component mixtures of glucose and ascorbic acid were observed to exhibit a response which was a simple addition of their individual responses.","Continuous flow instrumentation was designed, built, and tested. Reagent delivery and flow cell design were shown to play an important role in achieving reproducible and reliable data.","Ascorbic acid and dehydroascorbic acid were quantitated in the effluent of an HPLC, utilizing the chemiluminescence detector. Vitamin C tablets were assayed using the technique, yielding data that agreed to within 1-5% of titration results. A separation of glucose, glucuronic acid, uric acid, and ascorbic acid on an anion exchange column, at concentrations within their clinical ranges, was detected via the chemiluminescence method.","Made available in DSpace on 2014-12-13T20:10:58Z (GMT). No. of bitstreams: 1 8026608.pdf: 5944238 bytes, checksum: 189e2acddae7c8340ad836c7da221c0b (MD5) Previous issue date: 1980","Embargo set by: Seth Robbins for item 67412 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","212 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1980."],"dc:identifier":["http://hdl.handle.net/2142/67234","(UMI)AAI8026608"],"dc:language":["eng"],"dc:subject":["Chemistry, Analytical"],"dc:title":["Investigation of the Chemiluminescent Reaction Between Lucigenin and Organic Reductants and Its Application as a Detector for High Pressure Liquid Chromatography"],"dc:type":["text"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:57Z"}