{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/66984"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/66984","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"High Performance Reverse Phase Chromatography of Triglycerides, Cholesterol and Cholesteryl Esters and Its Application in the Analysis of Natural and Oxidized Oils","abstract":"Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1981.","abstract_html":"Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1981.","abstract_has_math":false,"creators":["El-Hamdy, Ali Hussein"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Food Science","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-13T19:47:51Z","date_published":"2014-12-13T19:47:51Z","updated_at":"2026-07-22T22:25:57Z","subjects":["Agriculture, Food Science and Technology"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8203452"],"render_values":[{"text":"(UMI)AAI8203452","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/66984","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["El-Hamdy, Ali Hussein"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-13T19:47:51Z","1981"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Food Science"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Agriculture, Food Science and Technology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8203452","http://hdl.handle.net/2142/66984"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1981.","Methods have been developed for the separation of triglycerides, cholesteryl and heated olive oil methyl esters using high performance reverse phase chromatography (HPRC). Preliminary studies on the separation of triglycerides showed the effect of packing characteristics (particle size bonded alkyl chain length, etc.) and the mobile phase composition and properties. The results indicate that better separation was achieved on a packing with an octadecyl hydrocarbon stationary phase bonded to a 5 (mu) silica gel particle than those accomplished on a shorter alkyl chain bonded to a larger particle silica gel. The optimum mobile phase was found to differ according to the packing used.","Resolution of critical pairs was accomplished on an octadecyl alkyl chain bonded column. Elution of these critical pairs was found to correlate with their molecular weight and shape and their degree of unsaturation. The elution sequence of molecules with the same Equivalent Carbon Number (ECN) starts with those with the highest number of double bonds and terminates with those with the lowest number of double bonds, with the lower ECN molecules eluting ahead of those with the higher ECN, and with cis isomers eluting ahead of their trans partners.","Complete separation of cyclic monomer esters, which are known to be toxic substances in heated fats, has been achieved using this system where no other single system was capable of such a separation.","This system of analysis was applied in the separation of the triglycerides of olive, soybean, palm and corn oils, lipoprotein and liver lipid cholesterol and cholesteryl esters; and the separation of thermally oxidized olive oil, methyl esters and the detection for the presence of cyclic monomer esters in these oils. Fractions of these esters were collected and subsequently analyzed by gas chromatography-mass spectrometry for their structure determination.","Made available in DSpace on 2014-12-13T19:47:51Z (GMT). No. of bitstreams: 1 8203452.pdf: 3365936 bytes, checksum: 9640197fad2f4d3c2ccb5bb680d03fbc (MD5) Previous issue date: 1981","Embargo set by: Seth Robbins for item 67162 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Open","125 p."]},{"key":"dc:title","label":"Title","values":["High Performance Reverse Phase Chromatography of Triglycerides, Cholesterol and Cholesteryl Esters and Its Application in the Analysis of Natural and Oxidized Oils"]}]}],"canonical_facts":{"dc:creator":["El-Hamdy, Ali Hussein"],"dc:date":["2014-12-13T19:47:51Z","1981"],"dc:description":["Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1981.","Methods have been developed for the separation of triglycerides, cholesteryl and heated olive oil methyl esters using high performance reverse phase chromatography (HPRC). Preliminary studies on the separation of triglycerides showed the effect of packing characteristics (particle size bonded alkyl chain length, etc.) and the mobile phase composition and properties. The results indicate that better separation was achieved on a packing with an octadecyl hydrocarbon stationary phase bonded to a 5 (mu) silica gel particle than those accomplished on a shorter alkyl chain bonded to a larger particle silica gel. The optimum mobile phase was found to differ according to the packing used.","Resolution of critical pairs was accomplished on an octadecyl alkyl chain bonded column. Elution of these critical pairs was found to correlate with their molecular weight and shape and their degree of unsaturation. The elution sequence of molecules with the same Equivalent Carbon Number (ECN) starts with those with the highest number of double bonds and terminates with those with the lowest number of double bonds, with the lower ECN molecules eluting ahead of those with the higher ECN, and with cis isomers eluting ahead of their trans partners.","Complete separation of cyclic monomer esters, which are known to be toxic substances in heated fats, has been achieved using this system where no other single system was capable of such a separation.","This system of analysis was applied in the separation of the triglycerides of olive, soybean, palm and corn oils, lipoprotein and liver lipid cholesterol and cholesteryl esters; and the separation of thermally oxidized olive oil, methyl esters and the detection for the presence of cyclic monomer esters in these oils. Fractions of these esters were collected and subsequently analyzed by gas chromatography-mass spectrometry for their structure determination.","Made available in DSpace on 2014-12-13T19:47:51Z (GMT). No. of bitstreams: 1 8203452.pdf: 3365936 bytes, checksum: 9640197fad2f4d3c2ccb5bb680d03fbc (MD5) Previous issue date: 1981","Embargo set by: Seth Robbins for item 67162 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Open","125 p."],"dc:identifier":["(UMI)AAI8203452","http://hdl.handle.net/2142/66984"],"dc:language":["eng"],"dc:subject":["Agriculture, Food Science and Technology"],"dc:title":["High Performance Reverse Phase Chromatography of Triglycerides, Cholesterol and Cholesteryl Esters and Its Application in the Analysis of Natural and Oxidized Oils"],"dc:type":["text"],"thesis:degree_discipline":["Food Science"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:57Z"}