{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/50567"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/50567","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Role of estrogen receptor α and oxidative stress response proteins in the central nervous system","abstract":"Made available in DSpace on 2014-09-16T17:23:57Z (GMT). No. of bitstreams: 3 Alicia_Dietrich.pdf: 2753057 bytes, checksum: 8fb8adbb98f08ad8b2b6b9a75a83fb3b (MD5) Dietrich_Alicia.docx: 4513759 bytes, checksum: 5942faabd81c4f91753707374679c5ad (MD5) license.txt: 4065 bytes, checksum: 32079de96bbbe5ce65fe5dddde9c1eb7 (MD5)","abstract_html":"Made available in DSpace on 2014-09-16T17:23:57Z (GMT). No. of bitstreams: 3 Alicia_Dietrich.pdf: 2753057 bytes, checksum: 8fb8adbb98f08ad8b2b6b9a75a83fb3b (MD5) Dietrich_Alicia.docx: 4513759 bytes, checksum: 5942faabd81c4f91753707374679c5ad (MD5) license.txt: 4065 bytes, checksum: 32079de96bbbe5ce65fe5dddde9c1eb7 (MD5)","abstract_has_math":false,"creators":["Dietrich, Alicia"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Molecular & Integrative Physi","degree_department":null,"school":null,"contributors":["Nardulli, Ann M.","Katzenellenbogen, Benita S.","Raetzman, Lori T.","Roy, Edward J."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-09-16T17:23:57Z","date_published":"2014-09-16T17:23:57Z","updated_at":"2026-07-22T22:25:40Z","subjects":["Oxidative stress","Estrogen receptor α","Cu/Zn superoxide dismutase","Amyotrophic lateral sclerosis","Apurinic endonuclease 1","Neuroprotection","Hypoxia","Estrogen"],"languages":["en"],"rights":["Copyright 2014 Alicia Dietrich"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/50567","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Nardulli, Ann M.","Katzenellenbogen, Benita S.","Raetzman, Lori T.","Roy, Edward J."]},{"key":"dc:creator","label":"Author","values":["Dietrich, Alicia"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-09-16T17:23:57Z","2014-08","2014-09-16"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Molecular & Integrative Physi"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Oxidative stress","Estrogen receptor α","Cu/Zn superoxide dismutase","Amyotrophic lateral sclerosis","Apurinic endonuclease 1","Neuroprotection","Hypoxia","Estrogen"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2014 Alicia Dietrich"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/50567"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Made available in DSpace on 2014-09-16T17:23:57Z (GMT). No. of bitstreams: 3 Alicia_Dietrich.pdf: 2753057 bytes, checksum: 8fb8adbb98f08ad8b2b6b9a75a83fb3b (MD5) Dietrich_Alicia.docx: 4513759 bytes, checksum: 5942faabd81c4f91753707374679c5ad (MD5) license.txt: 4065 bytes, checksum: 32079de96bbbe5ce65fe5dddde9c1eb7 (MD5)","Cellular metabolism results in the production of reactive oxygen species (ROS) as byproducts and can damage proteins, lipids, and DNA. Cells utilize a number of enzymes and small molecules to scavenge ROS and prevent and repair damage that has occurred. To better understand the role three of these proteins play in responding to ROS, we examined the activity of Cu/Zn superoxide dismutase, apurinic endonuclease, and estrogen receptor α in the central nervous system. Our studies demonstrate that oxidative protein damage in the spinal cord occurred prior to the appearance of motor symptoms in mice that express a mutant form of Cu/Zn superoxide dismutase and subsequently develop symptoms of amyotrophic lateral sclerosis. Additionally, we show increased apurinic endonuclease expression in the cerebral cortex of mice that had been ovariectomized, treated with 17β-estradiol, and then exposed to hypoxia. A decline in oxidative DNA damage occurred which we believe was the result of the 17β-estradiol-mediated increase in apurinic endonuclease expression. Finally, our work shows that estrogen receptor α protein expression in the cerebral cortex was stable in aging, hormonally intact females and this estrogen receptor α protein was functional. Altogether, these studies demonstrate the importance of understanding the impact of ROS-induced damage on human health and disease and provide insight to develop methods of prevention and treatment for neurodegenerative disorders.","Item withdrawn by Laura Spradlin (lspradl2@illinois.edu) on 2014-05-15T13:16:51Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Dietrich_Alicia.docx: 4513759 bytes, checksum: 5942faabd81c4f91753707374679c5ad (MD5) Dietrich_Alicia.pdf: 2753057 bytes, checksum: 8fb8adbb98f08ad8b2b6b9a75a83fb3b (MD5)"]},{"key":"dc:title","label":"Title","values":["Role of estrogen receptor α and oxidative stress response proteins in the central nervous system"]}]}],"canonical_facts":{"dc:contributor":["Nardulli, Ann M.","Katzenellenbogen, Benita S.","Raetzman, Lori T.","Roy, Edward J."],"dc:creator":["Dietrich, Alicia"],"dc:date":["2014-09-16T17:23:57Z","2014-08","2014-09-16"],"dc:description":["Made available in DSpace on 2014-09-16T17:23:57Z (GMT). No. of bitstreams: 3 Alicia_Dietrich.pdf: 2753057 bytes, checksum: 8fb8adbb98f08ad8b2b6b9a75a83fb3b (MD5) Dietrich_Alicia.docx: 4513759 bytes, checksum: 5942faabd81c4f91753707374679c5ad (MD5) license.txt: 4065 bytes, checksum: 32079de96bbbe5ce65fe5dddde9c1eb7 (MD5)","Cellular metabolism results in the production of reactive oxygen species (ROS) as byproducts and can damage proteins, lipids, and DNA. Cells utilize a number of enzymes and small molecules to scavenge ROS and prevent and repair damage that has occurred. To better understand the role three of these proteins play in responding to ROS, we examined the activity of Cu/Zn superoxide dismutase, apurinic endonuclease, and estrogen receptor α in the central nervous system. Our studies demonstrate that oxidative protein damage in the spinal cord occurred prior to the appearance of motor symptoms in mice that express a mutant form of Cu/Zn superoxide dismutase and subsequently develop symptoms of amyotrophic lateral sclerosis. Additionally, we show increased apurinic endonuclease expression in the cerebral cortex of mice that had been ovariectomized, treated with 17β-estradiol, and then exposed to hypoxia. A decline in oxidative DNA damage occurred which we believe was the result of the 17β-estradiol-mediated increase in apurinic endonuclease expression. Finally, our work shows that estrogen receptor α protein expression in the cerebral cortex was stable in aging, hormonally intact females and this estrogen receptor α protein was functional. Altogether, these studies demonstrate the importance of understanding the impact of ROS-induced damage on human health and disease and provide insight to develop methods of prevention and treatment for neurodegenerative disorders.","Item withdrawn by Laura Spradlin (lspradl2@illinois.edu) on 2014-05-15T13:16:51Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Dietrich_Alicia.docx: 4513759 bytes, checksum: 5942faabd81c4f91753707374679c5ad (MD5) Dietrich_Alicia.pdf: 2753057 bytes, checksum: 8fb8adbb98f08ad8b2b6b9a75a83fb3b (MD5)"],"dc:identifier":["http://hdl.handle.net/2142/50567"],"dc:language":["en"],"dc:rights":["Copyright 2014 Alicia Dietrich"],"dc:subject":["Oxidative stress","Estrogen receptor α","Cu/Zn superoxide dismutase","Amyotrophic lateral sclerosis","Apurinic endonuclease 1","Neuroprotection","Hypoxia","Estrogen"],"dc:title":["Role of estrogen receptor α and oxidative stress response proteins in the central nervous system"],"dc:type":["text"],"thesis:degree_discipline":["Molecular & Integrative Physi"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:40Z"}