{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/50410"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/50410","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Photonic crystal enhanced microscopy for imaging of live cell adhesion","abstract":"A form of microscopy based on the unique optical properties of a photonic crystal (PC) biosensor provides both label-free (LF) and enhanced fluorescence (EF) imaging modalities for the study of cell-surface interactions and nanoparticle-based assays. The imaging system utilizes an angle-tunable illumination source, either LED or laser, to achieve highly efficient coupling of incident light to PC optical resonances. Detection of the wavelength of resonantly reflected light from PC surfaces enable high-sensitivity label-free biosensing, whereas the enhanced electromagnetic (EM) fields associated with the resonances can be used to strongly improve the fluorescence output of fluorescent labels above PC surfaces. Photonic Crystal Enhanced Microscopy (PCEM) was applied to the study of murine dental stem cells by providing label-free, quantitative, submicron resolution, time-resolved images of the evolution of cell attachment and morphology during chemotaxis and drug-induced apoptosis. It enabled the monitoring of cell behavior with spatial resolution sufficient for observing intracellular attachment strength gradients. PCEM was also used to image fluorescence labeled cells, in which alternating on/off-resonance images was used to derive maps of the surface engagement of cell structures.","abstract_html":"A form of microscopy based on the unique optical properties of a photonic crystal (PC) biosensor provides both label-free (LF) and enhanced fluorescence (EF) imaging modalities for the study of cell-surface interactions and nanoparticle-based assays. The imaging system utilizes an angle-tunable illumination source, either LED or laser, to achieve highly efficient coupling of incident light to PC optical resonances. Detection of the wavelength of resonantly reflected light from PC surfaces enable high-sensitivity label-free biosensing, whereas the enhanced electromagnetic (EM) fields associated with the resonances can be used to strongly improve the fluorescence output of fluorescent labels above PC surfaces. Photonic Crystal Enhanced Microscopy (PCEM) was applied to the study of murine dental stem cells by providing label-free, quantitative, submicron resolution, time-resolved images of the evolution of cell attachment and morphology during chemotaxis and drug-induced apoptosis. It enabled the monitoring of cell behavior with spatial resolution sufficient for observing intracellular attachment strength gradients. PCEM was also used to image fluorescence labeled cells, in which alternating on/off-resonance images was used to derive maps of the surface engagement of cell structures.","abstract_has_math":false,"creators":["Chen, Weili"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Electrical & Computer Engr","degree_department":null,"school":null,"contributors":["Cunningham, Brian T."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-09-16T17:12:41Z","date_published":"2014-09-16T17:12:41Z","updated_at":"2026-07-22T22:25:40Z","subjects":["Biosensing","biomedical imaging","live cell"],"languages":["en"],"rights":["Copyright 2014 Weili Chen"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/50410","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Cunningham, Brian T."]},{"key":"dc:creator","label":"Author","values":["Chen, Weili"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-09-16T17:12:41Z","2016-09-22T20:59:19Z","2014-08","2014-09-16"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Electrical & Computer Engr"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biosensing","biomedical imaging","live cell"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2014 Weili Chen"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/50410"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["A form of microscopy based on the unique optical properties of a photonic crystal (PC) biosensor provides both label-free (LF) and enhanced fluorescence (EF) imaging modalities for the study of cell-surface interactions and nanoparticle-based assays. The imaging system utilizes an angle-tunable illumination source, either LED or laser, to achieve highly efficient coupling of incident light to PC optical resonances. Detection of the wavelength of resonantly reflected light from PC surfaces enable high-sensitivity label-free biosensing, whereas the enhanced electromagnetic (EM) fields associated with the resonances can be used to strongly improve the fluorescence output of fluorescent labels above PC surfaces. Photonic Crystal Enhanced Microscopy (PCEM) was applied to the study of murine dental stem cells by providing label-free, quantitative, submicron resolution, time-resolved images of the evolution of cell attachment and morphology during chemotaxis and drug-induced apoptosis. It enabled the monitoring of cell behavior with spatial resolution sufficient for observing intracellular attachment strength gradients. PCEM was also used to image fluorescence labeled cells, in which alternating on/off-resonance images was used to derive maps of the surface engagement of cell structures.","Item withdrawn by Laura Spradlin (lspradl2@illinois.edu) on 2014-07-21T20:30:58Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Chen_Weili.pdf: 1221194 bytes, checksum: 492c86b493a6221c019cacb11c9f4812 (MD5)","Made available in DSpace on 2014-09-16T17:12:41Z (GMT). No. of bitstreams: 2 Weili_Chen.pdf: 1220622 bytes, checksum: cdfa9801468e6f1294e2b539cf34682e (MD5) license.txt: 4059 bytes, checksum: 4a3a9bb2235a9edca8ca6f4e13688136 (MD5)","Embargo set by: Seth Robbins for item 50521 Lift date: 2016-09-16T17:13:01Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited Restriction Lifted for Item 50521 on 2016-09-22T20:59:19Z."]},{"key":"dc:title","label":"Title","values":["Photonic crystal enhanced microscopy for imaging of live cell adhesion"]}]}],"canonical_facts":{"dc:contributor":["Cunningham, Brian T."],"dc:creator":["Chen, Weili"],"dc:date":["2014-09-16T17:12:41Z","2016-09-22T20:59:19Z","2014-08","2014-09-16"],"dc:description":["A form of microscopy based on the unique optical properties of a photonic crystal (PC) biosensor provides both label-free (LF) and enhanced fluorescence (EF) imaging modalities for the study of cell-surface interactions and nanoparticle-based assays. The imaging system utilizes an angle-tunable illumination source, either LED or laser, to achieve highly efficient coupling of incident light to PC optical resonances. Detection of the wavelength of resonantly reflected light from PC surfaces enable high-sensitivity label-free biosensing, whereas the enhanced electromagnetic (EM) fields associated with the resonances can be used to strongly improve the fluorescence output of fluorescent labels above PC surfaces. Photonic Crystal Enhanced Microscopy (PCEM) was applied to the study of murine dental stem cells by providing label-free, quantitative, submicron resolution, time-resolved images of the evolution of cell attachment and morphology during chemotaxis and drug-induced apoptosis. It enabled the monitoring of cell behavior with spatial resolution sufficient for observing intracellular attachment strength gradients. PCEM was also used to image fluorescence labeled cells, in which alternating on/off-resonance images was used to derive maps of the surface engagement of cell structures.","Item withdrawn by Laura Spradlin (lspradl2@illinois.edu) on 2014-07-21T20:30:58Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Chen_Weili.pdf: 1221194 bytes, checksum: 492c86b493a6221c019cacb11c9f4812 (MD5)","Made available in DSpace on 2014-09-16T17:12:41Z (GMT). No. of bitstreams: 2 Weili_Chen.pdf: 1220622 bytes, checksum: cdfa9801468e6f1294e2b539cf34682e (MD5) license.txt: 4059 bytes, checksum: 4a3a9bb2235a9edca8ca6f4e13688136 (MD5)","Embargo set by: Seth Robbins for item 50521 Lift date: 2016-09-16T17:13:01Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited Restriction Lifted for Item 50521 on 2016-09-22T20:59:19Z."],"dc:identifier":["http://hdl.handle.net/2142/50410"],"dc:language":["en"],"dc:rights":["Copyright 2014 Weili Chen"],"dc:subject":["Biosensing","biomedical imaging","live cell"],"dc:title":["Photonic crystal enhanced microscopy for imaging of live cell adhesion"],"dc:type":["text"],"thesis:degree_discipline":["Electrical & Computer Engr"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:40Z"}