{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/46925"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/46925","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Construction and analysis of the miscanthus genespace","abstract":"Miscanthus and energy cane are closely related perennial grasses and candidate bioenergy crops. Both genomes create a challenge for variant discovery and genotyping because of their abundant repeats and the presence of a genome scale duplication with little subsequent divergence. In addition, the Miscanthus genome has high heterozygosity due to its self-incompatible breeding system. This complexity necessitates the discovery of sequence variants that distinguish paralogs from alleles, which are most frequent in the low-copy non-coding fraction of the genespace. To enrich for such sequences from Miscanthus genomes, we developed a sequence capture array using a solution-based hybridization method. The probe set was designed to capture exons and flanking intronic sequences predicted from alignment of Miscanthus transcriptome, fosmid, and genomic reads to the largely syntenic Sorghum genome. To facilitate haplotype discovery, we selected for large insert sizes that were subsequently sequenced at high depth as 100x75 bp paired-end reads. Chapter 1 reports the sequence capture of a doubled haploid Miscanthus sinensis plant and its parent, and chapter 2 reports the sequence capture of energy cane and two other Miscanthus species. This sequence capture design was able to distinguish variation at high depth and it was found that amplification is imperative to achieve this depth. Further analysis of the reads verified that Miscanthus has undergone recent whole genome duplication. Additionally, sequence capture serves as a useful tool for comparative genomics and provides strong evidence that Miscanthus can serve as a satisfactory reference to the closely related, yet highly complex genomes of Saccharum spp.","abstract_html":"Miscanthus and energy cane are closely related perennial grasses and candidate bioenergy crops. Both genomes create a challenge for variant discovery and genotyping because of their abundant repeats and the presence of a genome scale duplication with little subsequent divergence. In addition, the Miscanthus genome has high heterozygosity due to its self-incompatible breeding system. This complexity necessitates the discovery of sequence variants that distinguish paralogs from alleles, which are most frequent in the low-copy non-coding fraction of the genespace. To enrich for such sequences from Miscanthus genomes, we developed a sequence capture array using a solution-based hybridization method. The probe set was designed to capture exons and flanking intronic sequences predicted from alignment of Miscanthus transcriptome, fosmid, and genomic reads to the largely syntenic Sorghum genome. To facilitate haplotype discovery, we selected for large insert sizes that were subsequently sequenced at high depth as 100x75 bp paired-end reads. Chapter 1 reports the sequence capture of a doubled haploid Miscanthus sinensis plant and its parent, and chapter 2 reports the sequence capture of energy cane and two other Miscanthus species. This sequence capture design was able to distinguish variation at high depth and it was found that amplification is imperative to achieve this depth. Further analysis of the reads verified that Miscanthus has undergone recent whole genome duplication. Additionally, sequence capture serves as a useful tool for comparative genomics and provides strong evidence that Miscanthus can serve as a satisfactory reference to the closely related, yet highly complex genomes of Saccharum spp.","abstract_has_math":false,"creators":["Kirkpatrick, Jessica"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Bioinformatics","degree_department":null,"school":null,"contributors":["Moose, Stephen P."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-01-16T18:26:31Z","date_published":"2014-01-16T18:26:31Z","updated_at":"2026-07-22T22:25:38Z","subjects":["Miscanthus","sequence capture","Saccharum","variation","comparative genomics","genomics"],"languages":["en"],"rights":["Copyright 2013 Jessica Kirkpatrick"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/46925","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Moose, Stephen P."]},{"key":"dc:creator","label":"Author","values":["Kirkpatrick, Jessica"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-01-16T18:26:31Z","2016-01-16T11:00:39Z","2013-12"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Bioinformatics"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Miscanthus","sequence capture","Saccharum","variation","comparative genomics","genomics"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2013 Jessica Kirkpatrick"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/46925"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Miscanthus and energy cane are closely related perennial grasses and candidate bioenergy crops. Both genomes create a challenge for variant discovery and genotyping because of their abundant repeats and the presence of a genome scale duplication with little subsequent divergence. In addition, the Miscanthus genome has high heterozygosity due to its self-incompatible breeding system. This complexity necessitates the discovery of sequence variants that distinguish paralogs from alleles, which are most frequent in the low-copy non-coding fraction of the genespace. To enrich for such sequences from Miscanthus genomes, we developed a sequence capture array using a solution-based hybridization method. The probe set was designed to capture exons and flanking intronic sequences predicted from alignment of Miscanthus transcriptome, fosmid, and genomic reads to the largely syntenic Sorghum genome. To facilitate haplotype discovery, we selected for large insert sizes that were subsequently sequenced at high depth as 100x75 bp paired-end reads. Chapter 1 reports the sequence capture of a doubled haploid Miscanthus sinensis plant and its parent, and chapter 2 reports the sequence capture of energy cane and two other Miscanthus species. This sequence capture design was able to distinguish variation at high depth and it was found that amplification is imperative to achieve this depth. Further analysis of the reads verified that Miscanthus has undergone recent whole genome duplication. Additionally, sequence capture serves as a useful tool for comparative genomics and provides strong evidence that Miscanthus can serve as a satisfactory reference to the closely related, yet highly complex genomes of Saccharum spp.","Item withdrawn by Laura Spradlin (lspradl2@illinois.edu) on 2013-12-05T22:23:47Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Kirkpatrick_Jessica.docx: 10188025 bytes, checksum: 53127ec096137ab97226c031706ab4d0 (MD5) Kirkpatrick_Jessica.pdf: 5703079 bytes, checksum: 468be0df75dc55bc72d38c7b9b2feb16 (MD5)","Made available in DSpace on 2014-01-16T18:26:31Z (GMT). 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Both genomes create a challenge for variant discovery and genotyping because of their abundant repeats and the presence of a genome scale duplication with little subsequent divergence. In addition, the Miscanthus genome has high heterozygosity due to its self-incompatible breeding system. This complexity necessitates the discovery of sequence variants that distinguish paralogs from alleles, which are most frequent in the low-copy non-coding fraction of the genespace. To enrich for such sequences from Miscanthus genomes, we developed a sequence capture array using a solution-based hybridization method. The probe set was designed to capture exons and flanking intronic sequences predicted from alignment of Miscanthus transcriptome, fosmid, and genomic reads to the largely syntenic Sorghum genome. To facilitate haplotype discovery, we selected for large insert sizes that were subsequently sequenced at high depth as 100x75 bp paired-end reads. Chapter 1 reports the sequence capture of a doubled haploid Miscanthus sinensis plant and its parent, and chapter 2 reports the sequence capture of energy cane and two other Miscanthus species. This sequence capture design was able to distinguish variation at high depth and it was found that amplification is imperative to achieve this depth. Further analysis of the reads verified that Miscanthus has undergone recent whole genome duplication. Additionally, sequence capture serves as a useful tool for comparative genomics and provides strong evidence that Miscanthus can serve as a satisfactory reference to the closely related, yet highly complex genomes of Saccharum spp.","Item withdrawn by Laura Spradlin (lspradl2@illinois.edu) on 2013-12-05T22:23:47Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Kirkpatrick_Jessica.docx: 10188025 bytes, checksum: 53127ec096137ab97226c031706ab4d0 (MD5) Kirkpatrick_Jessica.pdf: 5703079 bytes, checksum: 468be0df75dc55bc72d38c7b9b2feb16 (MD5)","Made available in DSpace on 2014-01-16T18:26:31Z (GMT). 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