{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/46872"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/46872","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Persistence of Bacteroides ovatus under simulated sunlight irradiation","abstract":"The persistence of Bacteroides ovatus, a human-associated anaerobic fecal contamination indicator bacteria species, was evaluated under simulated sunlight exposure and conditions similar to surface water and seawater. By combining propidium monoazide (PMA) treatment and quantitative polymerase chain reaction (qPCR) detection, the decay rates of B. ovatus were determined in the presence of exogenous photosensitizers and salinity up to 39.5 parts per thousand at 27oC. UVB was found to be important for B.ovatus decay, averaging a four log10 of decay over 6 h of exposure without the presence of extracellular photosensitizers. The addition of NaNO2, an exogenous sensitizer producing hydroxyl radicals, did not significantly change the decay rate of B. ovatus, while the exogenous sensitizer algae organic matter (AOM) slowed down the decay of B. ovatus. At seawater salinity, the decay rate of B. ovatus is either slower than or as fast as that at freshwater salinity. Exogenous photosensitizers did not accelerate B. ovatus decay when being present alone in both low and high salinity solutions. Our study suggests that different B.ovatus persistence exists between light and dark treatment: if it is released into either the water or seawater environment in the evening, 50% of it will still be alive by the next morning; if it is released at noon, only 50% will be alive after mere 5 min of full spectrum irradiation on a clear day.","abstract_html":"The persistence of Bacteroides ovatus, a human-associated anaerobic fecal contamination indicator bacteria species, was evaluated under simulated sunlight exposure and conditions similar to surface water and seawater. By combining propidium monoazide (PMA) treatment and quantitative polymerase chain reaction (qPCR) detection, the decay rates of B. ovatus were determined in the presence of exogenous photosensitizers and salinity up to 39.5 parts per thousand at 27oC. UVB was found to be important for B.ovatus decay, averaging a four log10 of decay over 6 h of exposure without the presence of extracellular photosensitizers. The addition of NaNO2, an exogenous sensitizer producing hydroxyl radicals, did not significantly change the decay rate of B. ovatus, while the exogenous sensitizer algae organic matter (AOM) slowed down the decay of B. ovatus. At seawater salinity, the decay rate of B. ovatus is either slower than or as fast as that at freshwater salinity. Exogenous photosensitizers did not accelerate B. ovatus decay when being present alone in both low and high salinity solutions. Our study suggests that different B.ovatus persistence exists between light and dark treatment: if it is released into either the water or seawater environment in the evening, 50% of it will still be alive by the next morning; if it is released at noon, only 50% will be alive after mere 5 min of full spectrum irradiation on a clear day.","abstract_has_math":false,"creators":["Dong, Shengkun"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Environ Engr in Civil Engr","degree_department":null,"school":null,"contributors":["Nguyen, Thanh H."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-01-16T18:19:19Z","date_published":"2014-01-16T18:19:19Z","updated_at":"2026-07-22T22:25:38Z","subjects":["Bacteroides ovatus","natural organic matter","algae","hydroxyl radicals","salinity","simulated sunlight","Persistence"],"languages":["en"],"rights":["Copyright 2013 Shengkun Dong"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/46872","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Nguyen, Thanh H."]},{"key":"dc:creator","label":"Author","values":["Dong, Shengkun"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-01-16T18:19:19Z","2016-01-16T11:01:37Z","2013-12"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Environ Engr in Civil Engr"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Bacteroides ovatus","natural organic matter","algae","hydroxyl radicals","salinity","simulated sunlight","Persistence"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2013 Shengkun Dong"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/46872"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The persistence of Bacteroides ovatus, a human-associated anaerobic fecal contamination indicator bacteria species, was evaluated under simulated sunlight exposure and conditions similar to surface water and seawater. By combining propidium monoazide (PMA) treatment and quantitative polymerase chain reaction (qPCR) detection, the decay rates of B. ovatus were determined in the presence of exogenous photosensitizers and salinity up to 39.5 parts per thousand at 27oC. UVB was found to be important for B.ovatus decay, averaging a four log10 of decay over 6 h of exposure without the presence of extracellular photosensitizers. The addition of NaNO2, an exogenous sensitizer producing hydroxyl radicals, did not significantly change the decay rate of B. ovatus, while the exogenous sensitizer algae organic matter (AOM) slowed down the decay of B. ovatus. At seawater salinity, the decay rate of B. ovatus is either slower than or as fast as that at freshwater salinity. Exogenous photosensitizers did not accelerate B. ovatus decay when being present alone in both low and high salinity solutions. Our study suggests that different B.ovatus persistence exists between light and dark treatment: if it is released into either the water or seawater environment in the evening, 50% of it will still be alive by the next morning; if it is released at noon, only 50% will be alive after mere 5 min of full spectrum irradiation on a clear day.","Item withdrawn by Laura Spradlin (lspradl2@illinois.edu) on 2013-11-22T23:11:54Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Dong_Shengkun.docx: 542128 bytes, checksum: 21a2134664a5335f0ff8f595cbba6b30 (MD5) Dong_Shengkun.pdf: 1467111 bytes, checksum: 672d85602edcf742af180414673b714d (MD5)","Made available in DSpace on 2014-01-16T18:19:19Z (GMT). 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By combining propidium monoazide (PMA) treatment and quantitative polymerase chain reaction (qPCR) detection, the decay rates of B. ovatus were determined in the presence of exogenous photosensitizers and salinity up to 39.5 parts per thousand at 27oC. UVB was found to be important for B.ovatus decay, averaging a four log10 of decay over 6 h of exposure without the presence of extracellular photosensitizers. The addition of NaNO2, an exogenous sensitizer producing hydroxyl radicals, did not significantly change the decay rate of B. ovatus, while the exogenous sensitizer algae organic matter (AOM) slowed down the decay of B. ovatus. At seawater salinity, the decay rate of B. ovatus is either slower than or as fast as that at freshwater salinity. Exogenous photosensitizers did not accelerate B. ovatus decay when being present alone in both low and high salinity solutions. Our study suggests that different B.ovatus persistence exists between light and dark treatment: if it is released into either the water or seawater environment in the evening, 50% of it will still be alive by the next morning; if it is released at noon, only 50% will be alive after mere 5 min of full spectrum irradiation on a clear day.","Item withdrawn by Laura Spradlin (lspradl2@illinois.edu) on 2013-11-22T23:11:54Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Dong_Shengkun.docx: 542128 bytes, checksum: 21a2134664a5335f0ff8f595cbba6b30 (MD5) Dong_Shengkun.pdf: 1467111 bytes, checksum: 672d85602edcf742af180414673b714d (MD5)","Made available in DSpace on 2014-01-16T18:19:19Z (GMT). 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