Uniform biodegradable microparticle systems for protein delivery
type: CreativeWork
description: Therapeutic protein drugs are receiving increasing attention in pharmaceutical industry due to the fast development of biotechnology and genomics. Because of their low oral and transdermal bioavailabilities, fragility and short half-lives in vivo, novel and effective alternatives to deliver protein therapeutics are an important issue for protein therapeutics’ application. Biodegradable polymer devices have been utilized as a means to deliver drugs in a controlled and less invasive manner. Poly(lactide-co-glycolide) (PLG) and poly(lactic acid) (PLA) microparticles such as double-wall/monolithic microspheres and microcapsules have been heavily investigated for controlled delivery of small-molecule drugs as well as peptides, proteins and DNA. The size distribution of protein-loaded biodegradable polymer microparticles is a crucial factor for allowable routes of administration. Also, geometric structure of microparticles can influence the resulted release profile. In this project, by using the Precision Particle Fabrication (PPF) method, we produced uniform double-wall microspheres (DWMS) with a bovine serum albumin (BSA)-loaded PLG core and a drug-free PLA shell. Different PLG and PLA molecular weight and organic solvent combination to dissolve core and shell polymers (organic solvent configuration) were used to produce uniform DWMS with average size around 55 µm and ~10 µm shell thickness. By studying the in vitro release profiles, intraparticle protein distribution and particle morphology, we found using fast extracting shell solvent ethyl acetate, lower molecular weight PLG (Mw 4.2 kDa) core and relatively lower molecular weight PLA (Mw 43 kDa) shell resulted in spherical double-wall microspheres with higher protein loading, better core entrapment and postponed protein release compared to monolithic microsphere controls. Subsequently, using the optimized polymer and organic solvent configuration and increasing the shell PLA/core PLG mass ratio from 1.09 to 3.04, we …
abstract: Therapeutic protein drugs are receiving increasing attention in pharmaceutical industry due to the fast development of biotechnology and genomics. Because of their low oral and transdermal bioavailabilities, fragility and short half-lives in vivo, novel and effective alternatives to deliver protein therapeutics are an important issue for protein therapeutics’ application. Biodegradable polymer devices have been utilized as a means to deliver drugs in a controlled and less invasive manner. Poly(lactide-co-glycolide) (PLG) and poly(lactic acid) (PLA) microparticles such as double-wall/monolithic microspheres and microcapsules have been heavily investigated for controlled delivery of small-molecule drugs as well as peptides, proteins and DNA. The size distribution of protein-loaded biodegradable polymer microparticles is a crucial factor for allowable routes of administration. Also, geometric structure of microparticles can influence the resulted release profile. In this project, by using the Precision Particle Fabrication (PPF) method, we produced uniform double-wall microspheres (DWMS) with a bovine serum albumin (BSA)-loaded PLG core and a drug-free PLA shell. Different PLG and PLA molecular weight and organic solvent combination to dissolve core and shell polymers (organic solvent configuration) were used to produce uniform DWMS with average size around 55 µm and ~10 µm shell thickness. By studying the in vitro release profiles, intraparticle protein distribution and particle morphology, we found using fast extracting shell solvent ethyl acetate, lower molecular weight PLG (Mw 4.2 kDa) core and relatively lower molecular weight PLA (Mw 43 kDa) shell resulted in spherical double-wall microspheres with higher protein loading, better core entrapment and postponed protein release compared to monolithic microsphere controls. Subsequently, using the optimized polymer and organic solvent configuration and increasing the shell PLA/core PLG mass ratio from 1.09 to 3.04, we …
author: Xia, Yujie
language: en
keywords: Biodegradable Polymer, Poly(lactide-co-glycolide), Poly(lactic acid), Double-wall Microspheres, Microcapsules, Protein Delivery
date published: 2014-01-16T18:02:27Z
date modified: 2026-07-22T22:25:36Z
provider: University of Illinois - Urbana-Champaign
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Illinois at Urbana-Champaign

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2142/46785