{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/45631"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/45631","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Metagenomic characterization of Candidatatus Defluviicoccus tetraformis TFO71, a tetrad-forming organism, predominant in an anaerobic-aerobic membrane bioreactor with deteriorated biological phosphorus removal","abstract":"In an acetate-fed anaerobic-aerobic membrane bioreactor with deteriorated enhanced biological phosphorus removal (EBPR), Defluviicoccus-related tetrad-forming organisms (DTFO) were observed to predominate in the microbial community. Using metagenomics, a partial genome of the predominant DTFO, “Candidatus Defluviicoccus tetraformis TFO71,” was successfully constructed and characterized. Examining the genome confirmed the presence of genes related to the synthesis and degradation of glycogen and polyhydroxyalkanoate (PHA), which function as energy and carbon storage compounds. Both TFO71 and Candidatus Accumulibacter phosphatis (CAP) UW-1, a representative polyphosphate-accumulating organism (PAO), have PHA metabolism-related genes with high homology, but TFO71 has unique genes for PHA synthesis, gene regulation, and granule management. We further discovered genes encoding DTFO polyphosphate (polyP) synthesis, suggesting that TFO71 may synthesize polyP under untested conditions. However, TFO71 may not activate these genes under EBPR conditions because the retrieved genome does not contain inorganic phosphate transporters that are characteristic of PAOs (CAP UW-1, Microlunatus phosphovorus NM-1, and Tetrasphaera species). As a first step in characterizing EBPR-associated DTFO metabolism, this study identifies important differences between TFO and PAO that may contribute to EBPR community competition and deterioration.","abstract_html":"In an acetate-fed anaerobic-aerobic membrane bioreactor with deteriorated enhanced biological phosphorus removal (EBPR), Defluviicoccus-related tetrad-forming organisms (DTFO) were observed to predominate in the microbial community. Using metagenomics, a partial genome of the predominant DTFO, “Candidatus Defluviicoccus tetraformis TFO71,” was successfully constructed and characterized. Examining the genome confirmed the presence of genes related to the synthesis and degradation of glycogen and polyhydroxyalkanoate (PHA), which function as energy and carbon storage compounds. Both TFO71 and Candidatus Accumulibacter phosphatis (CAP) UW-1, a representative polyphosphate-accumulating organism (PAO), have PHA metabolism-related genes with high homology, but TFO71 has unique genes for PHA synthesis, gene regulation, and granule management. We further discovered genes encoding DTFO polyphosphate (polyP) synthesis, suggesting that TFO71 may synthesize polyP under untested conditions. However, TFO71 may not activate these genes under EBPR conditions because the retrieved genome does not contain inorganic phosphate transporters that are characteristic of PAOs (CAP UW-1, Microlunatus phosphovorus NM-1, and Tetrasphaera species). As a first step in characterizing EBPR-associated DTFO metabolism, this study identifies important differences between TFO and PAO that may contribute to EBPR community competition and deterioration.","abstract_has_math":false,"creators":["Nobu, Masaru"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Environ Engr in Civil Engr","degree_department":null,"school":null,"contributors":["Liu, Wen-Tso"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-08-22T16:56:02Z","date_published":"2013-08-22T16:56:02Z","updated_at":"2026-07-22T22:25:36Z","subjects":["Enhanced biological phosphorus removal","Defluviicoccus","tetrad-forming organisms","glycogen-accumulating organisms"],"languages":["en"],"rights":["Copyright 2013 Masaru Nobu"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/45631","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Liu, Wen-Tso"]},{"key":"dc:creator","label":"Author","values":["Nobu, Masaru"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2013-08-22T16:56:02Z","2015-08-22T10:00:26Z","2013-08"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Environ Engr in Civil Engr"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Enhanced biological phosphorus removal","Defluviicoccus","tetrad-forming organisms","glycogen-accumulating organisms"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2013 Masaru Nobu"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/45631"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["In an acetate-fed anaerobic-aerobic membrane bioreactor with deteriorated enhanced biological phosphorus removal (EBPR), Defluviicoccus-related tetrad-forming organisms (DTFO) were observed to predominate in the microbial community. Using metagenomics, a partial genome of the predominant DTFO, “Candidatus Defluviicoccus tetraformis TFO71,” was successfully constructed and characterized. Examining the genome confirmed the presence of genes related to the synthesis and degradation of glycogen and polyhydroxyalkanoate (PHA), which function as energy and carbon storage compounds. Both TFO71 and Candidatus Accumulibacter phosphatis (CAP) UW-1, a representative polyphosphate-accumulating organism (PAO), have PHA metabolism-related genes with high homology, but TFO71 has unique genes for PHA synthesis, gene regulation, and granule management. We further discovered genes encoding DTFO polyphosphate (polyP) synthesis, suggesting that TFO71 may synthesize polyP under untested conditions. However, TFO71 may not activate these genes under EBPR conditions because the retrieved genome does not contain inorganic phosphate transporters that are characteristic of PAOs (CAP UW-1, Microlunatus phosphovorus NM-1, and Tetrasphaera species). As a first step in characterizing EBPR-associated DTFO metabolism, this study identifies important differences between TFO and PAO that may contribute to EBPR community competition and deterioration.","Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2013-06-28T14:37:31Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Nobu_Masaru.pdf: 7767411 bytes, checksum: 4365353096aae70cbaaedda61560e9f2 (MD5)","Made available in DSpace on 2013-08-22T16:56:02Z (GMT). No. of bitstreams: 2 Masaru_Nobu.pdf: 7753753 bytes, checksum: 61689ea38fd190d35018800e78ff7647 (MD5) license.txt: 4058 bytes, checksum: d80fd9322ee87fcc4060766e67955737 (MD5)","Restriction data tranferred 2014-07-01T11:36:26-05:00 Original Data Group with Access Administrator Release Date: 2015-08-22 11:57:26 UTC Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Item marked as restricted to the 'Administrator' Group (id=1) by Seth Robbins (srobbins@illinois.edu) on 2013-08-22T16:57:33Z Item is restricted until 2015-08-22T16:57:26Z","Limited Restriction Lifted for Item 45613 on 2015-08-22T10:00:26Z."]},{"key":"dc:title","label":"Title","values":["Metagenomic characterization of Candidatatus Defluviicoccus tetraformis TFO71, a tetrad-forming organism, predominant in an anaerobic-aerobic membrane bioreactor with deteriorated biological phosphorus removal"]}]}],"canonical_facts":{"dc:contributor":["Liu, Wen-Tso"],"dc:creator":["Nobu, Masaru"],"dc:date":["2013-08-22T16:56:02Z","2015-08-22T10:00:26Z","2013-08"],"dc:description":["In an acetate-fed anaerobic-aerobic membrane bioreactor with deteriorated enhanced biological phosphorus removal (EBPR), Defluviicoccus-related tetrad-forming organisms (DTFO) were observed to predominate in the microbial community. Using metagenomics, a partial genome of the predominant DTFO, “Candidatus Defluviicoccus tetraformis TFO71,” was successfully constructed and characterized. Examining the genome confirmed the presence of genes related to the synthesis and degradation of glycogen and polyhydroxyalkanoate (PHA), which function as energy and carbon storage compounds. Both TFO71 and Candidatus Accumulibacter phosphatis (CAP) UW-1, a representative polyphosphate-accumulating organism (PAO), have PHA metabolism-related genes with high homology, but TFO71 has unique genes for PHA synthesis, gene regulation, and granule management. We further discovered genes encoding DTFO polyphosphate (polyP) synthesis, suggesting that TFO71 may synthesize polyP under untested conditions. However, TFO71 may not activate these genes under EBPR conditions because the retrieved genome does not contain inorganic phosphate transporters that are characteristic of PAOs (CAP UW-1, Microlunatus phosphovorus NM-1, and Tetrasphaera species). As a first step in characterizing EBPR-associated DTFO metabolism, this study identifies important differences between TFO and PAO that may contribute to EBPR community competition and deterioration.","Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2013-06-28T14:37:31Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Nobu_Masaru.pdf: 7767411 bytes, checksum: 4365353096aae70cbaaedda61560e9f2 (MD5)","Made available in DSpace on 2013-08-22T16:56:02Z (GMT). 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