{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/45574"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/45574","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Xenobiotic detoxification in the navel orangeworm Amyelois transitella (Lepidoptera: Pyralidae)","abstract":"The highly polyphagous navel orangeworm, Amyelois transitella, is a major pest of high-value tree crops such as pistachios and almonds. These non-native crops have been grown commercially in the United States for less than 150 years, and the acquisition by the navel orangeworm of these and other novel hosts may have been facilitated by its metabolic capacity to detoxify xenobiotics. Using Illumina RNA sequencing, I characterized the cytochrome P450 genes transcribed in the midgut of the caterpillar. A total of 319 million paired-end reads were sequenced from larval midgut tissue, and these were assembled de novo into 186,000 contigs. Identification of putative P450s in this assembly was carried out using BLAST homology. As detoxification of phytochemicals, mycotoxins, and pesticides is mediated at least in part by P540 enzymes, developing genomic resources for characterizing P450s and elucidating their function in this insect will aid in management efforts for this pest. An important component of elucidating P450-mediated detoxification in this highly polyphagous species is to understand how ingestion of multiple types of toxins influences detoxification. One pyrethroid pesticide used in almond orchards, α-cypermethrin, is likely detoxified by P450 genes transcribed in the navel orangeworm midgut. Dietary exposure to two phytochemicals found in almonds and pistachios, chlorogenic acid and quercetin, increases survival of navel orangeworms exposed to α-cypermethrin. These phytochemicals may decrease toxicity of the pesticide by inducing the P450s involved in its detoxification. Decreased effectiveness of α-cypermethrin is a concern to almond and pistachio growers. Characterization of the P450s involved in pesticide detoxification in the navel orangeworm on its multiple host plants can inform pesticide management strategies for this pest.","abstract_html":"The highly polyphagous navel orangeworm, Amyelois transitella, is a major pest of high-value tree crops such as pistachios and almonds. These non-native crops have been grown commercially in the United States for less than 150 years, and the acquisition by the navel orangeworm of these and other novel hosts may have been facilitated by its metabolic capacity to detoxify xenobiotics. Using Illumina RNA sequencing, I characterized the cytochrome P450 genes transcribed in the midgut of the caterpillar. A total of 319 million paired-end reads were sequenced from larval midgut tissue, and these were assembled de novo into 186,000 contigs. Identification of putative P450s in this assembly was carried out using BLAST homology. As detoxification of phytochemicals, mycotoxins, and pesticides is mediated at least in part by P540 enzymes, developing genomic resources for characterizing P450s and elucidating their function in this insect will aid in management efforts for this pest. An important component of elucidating P450-mediated detoxification in this highly polyphagous species is to understand how ingestion of multiple types of toxins influences detoxification. One pyrethroid pesticide used in almond orchards, α-cypermethrin, is likely detoxified by P450 genes transcribed in the navel orangeworm midgut. Dietary exposure to two phytochemicals found in almonds and pistachios, chlorogenic acid and quercetin, increases survival of navel orangeworms exposed to α-cypermethrin. These phytochemicals may decrease toxicity of the pesticide by inducing the P450s involved in its detoxification. Decreased effectiveness of α-cypermethrin is a concern to almond and pistachio growers. Characterization of the P450s involved in pesticide detoxification in the navel orangeworm on its multiple host plants can inform pesticide management strategies for this pest.","abstract_has_math":false,"creators":["Noble, Katherine"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Entomology","degree_department":null,"school":null,"contributors":["Berenbaum, May R."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-08-22T16:48:17Z","date_published":"2013-08-22T16:48:17Z","updated_at":"2026-07-22T22:25:36Z","subjects":["Cytochrome P450","Detoxification","Lepidoptera: Amyelois transitella: Midgut Transcriptome"],"languages":["en"],"rights":["Copyright 2013 Katherine Noble"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/45574","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Berenbaum, May R."]},{"key":"dc:creator","label":"Author","values":["Noble, Katherine"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2013-08-22T16:48:17Z","2015-08-22T10:00:50Z","2013-08"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Entomology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Cytochrome P450","Detoxification","Lepidoptera: Amyelois transitella: Midgut Transcriptome"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2013 Katherine Noble"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/45574"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The highly polyphagous navel orangeworm, Amyelois transitella, is a major pest of high-value tree crops such as pistachios and almonds. These non-native crops have been grown commercially in the United States for less than 150 years, and the acquisition by the navel orangeworm of these and other novel hosts may have been facilitated by its metabolic capacity to detoxify xenobiotics. Using Illumina RNA sequencing, I characterized the cytochrome P450 genes transcribed in the midgut of the caterpillar. A total of 319 million paired-end reads were sequenced from larval midgut tissue, and these were assembled de novo into 186,000 contigs. Identification of putative P450s in this assembly was carried out using BLAST homology. As detoxification of phytochemicals, mycotoxins, and pesticides is mediated at least in part by P540 enzymes, developing genomic resources for characterizing P450s and elucidating their function in this insect will aid in management efforts for this pest. An important component of elucidating P450-mediated detoxification in this highly polyphagous species is to understand how ingestion of multiple types of toxins influences detoxification. One pyrethroid pesticide used in almond orchards, α-cypermethrin, is likely detoxified by P450 genes transcribed in the navel orangeworm midgut. Dietary exposure to two phytochemicals found in almonds and pistachios, chlorogenic acid and quercetin, increases survival of navel orangeworms exposed to α-cypermethrin. These phytochemicals may decrease toxicity of the pesticide by inducing the P450s involved in its detoxification. Decreased effectiveness of α-cypermethrin is a concern to almond and pistachio growers. Characterization of the P450s involved in pesticide detoxification in the navel orangeworm on its multiple host plants can inform pesticide management strategies for this pest.","Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2013-07-09T21:39:22Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Noble_Katherine.docx: 1598243 bytes, checksum: 68475abf7497157753c4f3d1f09a2479 (MD5) Noble_Katherine.pdf: 1245803 bytes, checksum: a176d1608e6041ca07fe172b5e975085 (MD5)","Made available in DSpace on 2013-08-22T16:48:17Z (GMT). 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These non-native crops have been grown commercially in the United States for less than 150 years, and the acquisition by the navel orangeworm of these and other novel hosts may have been facilitated by its metabolic capacity to detoxify xenobiotics. Using Illumina RNA sequencing, I characterized the cytochrome P450 genes transcribed in the midgut of the caterpillar. A total of 319 million paired-end reads were sequenced from larval midgut tissue, and these were assembled de novo into 186,000 contigs. Identification of putative P450s in this assembly was carried out using BLAST homology. As detoxification of phytochemicals, mycotoxins, and pesticides is mediated at least in part by P540 enzymes, developing genomic resources for characterizing P450s and elucidating their function in this insect will aid in management efforts for this pest. An important component of elucidating P450-mediated detoxification in this highly polyphagous species is to understand how ingestion of multiple types of toxins influences detoxification. One pyrethroid pesticide used in almond orchards, α-cypermethrin, is likely detoxified by P450 genes transcribed in the navel orangeworm midgut. Dietary exposure to two phytochemicals found in almonds and pistachios, chlorogenic acid and quercetin, increases survival of navel orangeworms exposed to α-cypermethrin. These phytochemicals may decrease toxicity of the pesticide by inducing the P450s involved in its detoxification. Decreased effectiveness of α-cypermethrin is a concern to almond and pistachio growers. Characterization of the P450s involved in pesticide detoxification in the navel orangeworm on its multiple host plants can inform pesticide management strategies for this pest.","Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2013-07-09T21:39:22Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Noble_Katherine.docx: 1598243 bytes, checksum: 68475abf7497157753c4f3d1f09a2479 (MD5) Noble_Katherine.pdf: 1245803 bytes, checksum: a176d1608e6041ca07fe172b5e975085 (MD5)","Made available in DSpace on 2013-08-22T16:48:17Z (GMT). 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