Enhancing human health promoting activity through the regulation of the methyl jasmonate mediated glucosinolate biosynthesis in Brassica oleracea
type: CreativeWork
description: Brassica oleracea vegetables are recognized as functional foods that contain various phytochemicals such as glucosinolates (GS) and flavonoids that have health-promoting bioactivity. Recent data suggest that methyl jasmonic acid (MeJA) can increase concentrations of GS and polyphenolics in Brassica plants. In Chapter 2 tissue/organ specific responses to MeJA treatments were investigated in five cultivars of broccoli and two cultivars of kale in field plots over two years, MeJA treatments significantly increased total phenolics and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) antioxidant activity of kale leaf tissues, but had no effect on phenolics of broccoli florets. Correlation of growing degree days, precipitation per day and solar radiation with phenolic concentrations suggest that these weather related factors are associated with the enhancement of phenolics and tissue ABTS antioxidant activity. In order to evaluate if MeJA treatment can enhance induction of quinone reductase activity, an anticancer biomarker of broccoli floret extracts, MeJA treatments were applied to five broccoli cultivars in each of two years under field conditions (Chapter 3). Sulforaphane, phenethyl isothiocyanate, and hydrolysis products derived from neoglucobrassicin were significantly increased by MeJA treatment. Sulforaphane, N-methoxyindole-3-carbinol (NI3C), and neoascorbigen showed significant correlations with QR activity in hydrolysed broccoli extracts. Although sulforaphane is a known QR inducer, there is only one published report about QR activity of hydrolysis products of neoglucobrassicin (Haack et al., 2010). The concentration required for doubling specific QR activity (CD value) was calculated to be 35 and 38 µM for NI3C and neoascorbigen, respectively. The CD value of sulforaphane was previously estimated to be 0.2 µM. Given the QR inducing potency and increased amount of isothiocyanate hydrolysis product from glucoraphanin, sulforaphane is considered to…
abstract: Brassica oleracea vegetables are recognized as functional foods that contain various phytochemicals such as glucosinolates (GS) and flavonoids that have health-promoting bioactivity. Recent data suggest that methyl jasmonic acid (MeJA) can increase concentrations of GS and polyphenolics in Brassica plants. In Chapter 2 tissue/organ specific responses to MeJA treatments were investigated in five cultivars of broccoli and two cultivars of kale in field plots over two years, MeJA treatments significantly increased total phenolics and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) antioxidant activity of kale leaf tissues, but had no effect on phenolics of broccoli florets. Correlation of growing degree days, precipitation per day and solar radiation with phenolic concentrations suggest that these weather related factors are associated with the enhancement of phenolics and tissue ABTS antioxidant activity. In order to evaluate if MeJA treatment can enhance induction of quinone reductase activity, an anticancer biomarker of broccoli floret extracts, MeJA treatments were applied to five broccoli cultivars in each of two years under field conditions (Chapter 3). Sulforaphane, phenethyl isothiocyanate, and hydrolysis products derived from neoglucobrassicin were significantly increased by MeJA treatment. Sulforaphane, N-methoxyindole-3-carbinol (NI3C), and neoascorbigen showed significant correlations with QR activity in hydrolysed broccoli extracts. Although sulforaphane is a known QR inducer, there is only one published report about QR activity of hydrolysis products of neoglucobrassicin (Haack et al., 2010). The concentration required for doubling specific QR activity (CD value) was calculated to be 35 and 38 µM for NI3C and neoascorbigen, respectively. The CD value of sulforaphane was previously estimated to be 0.2 µM. Given the QR inducing potency and increased amount of isothiocyanate hydrolysis product from glucoraphanin, sulforaphane is considered to…
author: Ku, Kang Mo
language: en
keywords: Brassica oleracea, Glucosinolate, Flavonoid, Quinone reductase, Antioxidant activity, Pre-harvest, Post-harvest, Methyl jasmonate (MeJa), Broccoli, Kale, Cauliflower
date published: 2013-08-22T16:47:50Z
date modified: 2026-07-22T22:25:36Z
provider: University of Illinois - Urbana-Champaign
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Illinois at Urbana-Champaign

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2142/45560