{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/44771"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/44771","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Photonic crystal enhanced microscopy for the characterization of cell attachment","abstract":"Restriction data tranferred 2014-07-01T11:16:49-05:00 Original Data Group with Access Administrator Release Date: 2015-05-28 14:21:22 UTC Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","abstract_html":"Restriction data tranferred 2014-07-01T11:16:49-05:00 Original Data Group with Access Administrator Release Date: 2015-05-28 14:21:22 UTC Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","abstract_has_math":false,"creators":["Lidstone, Erich"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Bioengineering","degree_department":null,"school":null,"contributors":["Cunningham, Brian T.","Schook, Lawrence B.","Popescu, Gabriel","Underhill, Gregory"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-05-28T19:19:15Z","date_published":"2013-05-28T19:19:15Z","updated_at":"2026-07-22T22:25:34Z","subjects":["photonic","photonic crystal","biosensor","label-free","optical","label-free cell attachment","cell adhesion","attachment density","guided mode resonance filter"],"languages":["en"],"rights":["Copyright 2013 Erich Lidstone"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/44771","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Cunningham, Brian T.","Schook, Lawrence B.","Popescu, Gabriel","Underhill, Gregory"]},{"key":"dc:creator","label":"Author","values":["Lidstone, Erich"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2013-05-28T19:19:15Z","2015-05-28T10:02:31Z","2013-05"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Bioengineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["photonic","photonic crystal","biosensor","label-free","optical","label-free cell attachment","cell adhesion","attachment density","guided mode resonance filter"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2013 Erich Lidstone"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/44771"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Restriction data tranferred 2014-07-01T11:16:49-05:00 Original Data Group with Access Administrator Release Date: 2015-05-28 14:21:22 UTC Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","As basic science research has increased in both complexity and scale, so too has the importance of cost-effective and generalizable techniques for the interrogation of cells and tissues. Over the past few decades, several technologies have been leveraged to meet this demand, recently including photonic crystal (PC) biosensors. This work describes the application of PC biosensors to cell microscopy, resulting in a label-free imaging technique capable of measuring cell attachment and attachment modulation. The approach uses a photonic crystal optical resonator surface incorporated into conventional microplate wells and a microscope-based detection instrument that measures shifts in the resonant coupling conditions caused by localized changes in dielectric permittivity at the cell-sensor interface. Four model systems are demonstrated for studying cancer cells, primary cardiac muscle cells, and stem cells. Each experiment yielded information regarding cell attachment density without the use of potentially cytotoxic labels, enabling study of processes including growth, development, differentiation, and death in the same cells for periods lasting several days. After demonstrating this technology in several systems typical of cell biology problems, PC biosensors were applied toward the investigation of tumor immunity. In particular, it has remained difficult for immunologists to study how tumor cells interact with components of the innate immune system, especially in the context of specific apoptotic recognition. The final chapter details the use of PC biosensors in detecting these types of interactions between two distinct cell types, the first reported use of label-free biosensors to detect such cellular changes.","Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2013-04-11T14:45:18Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Lidstone_Thesis_Draft2.pdf: 7070906 bytes, checksum: 1cad8489d6bc8c81e9b4eab72c81a796 (MD5)","Made available in DSpace on 2013-05-28T19:19:15Z (GMT). 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Over the past few decades, several technologies have been leveraged to meet this demand, recently including photonic crystal (PC) biosensors. This work describes the application of PC biosensors to cell microscopy, resulting in a label-free imaging technique capable of measuring cell attachment and attachment modulation. The approach uses a photonic crystal optical resonator surface incorporated into conventional microplate wells and a microscope-based detection instrument that measures shifts in the resonant coupling conditions caused by localized changes in dielectric permittivity at the cell-sensor interface. Four model systems are demonstrated for studying cancer cells, primary cardiac muscle cells, and stem cells. Each experiment yielded information regarding cell attachment density without the use of potentially cytotoxic labels, enabling study of processes including growth, development, differentiation, and death in the same cells for periods lasting several days. After demonstrating this technology in several systems typical of cell biology problems, PC biosensors were applied toward the investigation of tumor immunity. In particular, it has remained difficult for immunologists to study how tumor cells interact with components of the innate immune system, especially in the context of specific apoptotic recognition. The final chapter details the use of PC biosensors in detecting these types of interactions between two distinct cell types, the first reported use of label-free biosensors to detect such cellular changes.","Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2013-04-11T14:45:18Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Lidstone_Thesis_Draft2.pdf: 7070906 bytes, checksum: 1cad8489d6bc8c81e9b4eab72c81a796 (MD5)","Made available in DSpace on 2013-05-28T19:19:15Z (GMT). No. of bitstreams: 2 Erich_Lidstone.pdf: 7316806 bytes, checksum: 22eb77bf43e57f1ee202af2edbc5f810 (MD5) license.txt: 4063 bytes, checksum: b7c2927ebf3ed19a61258859b09b3615 (MD5)","Item marked as restricted to the 'Administrator' Group (id=1) by Seth Robbins (srobbins@illinois.edu) on 2013-05-28T19:21:38Z Item is restricted until 2015-05-28T19:21:22Z","Limited Restriction Lifted for Item 44746 on 2015-05-28T10:02:31Z."],"dc:identifier":["http://hdl.handle.net/2142/44771"],"dc:language":["en"],"dc:rights":["Copyright 2013 Erich Lidstone"],"dc:subject":["photonic","photonic crystal","biosensor","label-free","optical","label-free cell attachment","cell adhesion","attachment density","guided mode resonance filter"],"dc:title":["Photonic crystal enhanced microscopy for the characterization of cell attachment"],"dc:type":["text"],"thesis:degree_discipline":["Bioengineering"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:34Z"}