{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/44448"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/44448","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"The development of a skeletal muscle bio-actuator using 3-D stereolithography","abstract":"Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2013-04-22T15:23:45Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Cvetkovic_Caroline.pdf: 9089391 bytes, checksum: 3bca9a127ed7823923b3518da57bc11a (MD5)","abstract_html":"Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2013-04-22T15:23:45Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Cvetkovic_Caroline.pdf: 9089391 bytes, checksum: 3bca9a127ed7823923b3518da57bc11a (MD5)","abstract_has_math":false,"creators":["Cvetkovic, Caroline"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Bioengineering","degree_department":null,"school":null,"contributors":["Bashir, Rashid"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-05-24T22:16:30Z","date_published":"2013-05-24T22:16:30Z","updated_at":"2026-07-22T22:25:34Z","subjects":["skeletal muscle","bio-actuator","stereolithography"],"languages":["en"],"rights":["Copyright 2013 Caroline Elizabeth Cvetkovic"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/44448","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Bashir, Rashid"]},{"key":"dc:creator","label":"Author","values":["Cvetkovic, Caroline"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2013-05-24T22:16:30Z","2015-05-24T10:01:49Z","2013-05"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Bioengineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["skeletal muscle","bio-actuator","stereolithography"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2013 Caroline Elizabeth Cvetkovic"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/44448"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2013-04-22T15:23:45Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Cvetkovic_Caroline.pdf: 9089391 bytes, checksum: 3bca9a127ed7823923b3518da57bc11a (MD5)","Over the past decade, a new scientific discipline has emerged, integrating mechanics with biology to create complex engineered living systems. The building blocks – different cell types in an instructive environment – can be assembled in various ways to promote the emergence (or natural evolution and interaction) of the cells in a system with well-defined functionality. These functions could include sensing, information processing, protein expression, and actuation, among countless others. This Thesis presents a novel cellular system capable of actuation and fabricated using cells and hydrogels. A stereolithographic 3D printing technique (SLA) was used to create a hydrogel backbone made of a beam connecting two pillars that supports a muscle strip created from skeletal muscle cells in a fibrin-based matrix. The entire device is termed a “bio-bot,” or biological robot. Contraction of the cells within the muscle strip produced enough force to move the pillars and displace the bio-bot on a surface in a liquid medium. This Thesis is focused on the development and characterization (mechanical and biological) of the skeletal muscle-based biological actuator. The use of the SLA allowed for easy modifications of the polymerized part’s geometry and material properties. Increasing the energy dose of polymerization produced a stiffer beam that restricted bending and increased the passive tension in the muscle strip. 15-19 days after cell seeding, the bio-bots displayed spontaneous contraction that resulted in a net displacement of up to ~6 mm in 10 minutes. During this time span, a maximum velocity of over 1890 μm/min was achieved. Future plans are focused on controlling the activity of the bio-bot using optogenetics.","Made available in DSpace on 2013-05-24T22:16:30Z (GMT). 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The building blocks – different cell types in an instructive environment – can be assembled in various ways to promote the emergence (or natural evolution and interaction) of the cells in a system with well-defined functionality. These functions could include sensing, information processing, protein expression, and actuation, among countless others. This Thesis presents a novel cellular system capable of actuation and fabricated using cells and hydrogels. A stereolithographic 3D printing technique (SLA) was used to create a hydrogel backbone made of a beam connecting two pillars that supports a muscle strip created from skeletal muscle cells in a fibrin-based matrix. The entire device is termed a “bio-bot,” or biological robot. Contraction of the cells within the muscle strip produced enough force to move the pillars and displace the bio-bot on a surface in a liquid medium. This Thesis is focused on the development and characterization (mechanical and biological) of the skeletal muscle-based biological actuator. The use of the SLA allowed for easy modifications of the polymerized part’s geometry and material properties. Increasing the energy dose of polymerization produced a stiffer beam that restricted bending and increased the passive tension in the muscle strip. 15-19 days after cell seeding, the bio-bots displayed spontaneous contraction that resulted in a net displacement of up to ~6 mm in 10 minutes. During this time span, a maximum velocity of over 1890 μm/min was achieved. Future plans are focused on controlling the activity of the bio-bot using optogenetics.","Made available in DSpace on 2013-05-24T22:16:30Z (GMT). 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