{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/42397"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/42397","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Characterization of the intestinal microbiome and lactobacilli community: influence of dietary and environmental factors","abstract":"The human gastrointestinal (GI) tract is colonized by a dense and diverse bacterial community called the commensal microbiota, which plays an important role in overall health of individuals. However, due to the extreme complexity of microbiota composition and limits of research tools, there were many challenges in identify and compare individual members of the microbiota, and the impacts of multiple factors on the development of microbiota were poorly understood. This is a comprehensive study on applying traditional culture dependent methods, as well as the latest developed molecular biological tools, in neonatal piglets’ intestinal microbiome and lactobacilli community structure research. In Chapter 2, the latest progress of methods used in microbiota research were described and compared, the known impact of different environmental and dietary factors were discussed. In Chapter 3, a new protocol of Terminal restriction fragment length polymorphism (T-RFLP) analysis was developed to facilitate lactobacilli composition study. In Chapter 4, both culture dependent methods and the novel T-RFLP tools were used to reveal the impact of piglet age and route of delivery on ileal Lactobacillus diversity. In Chapter 5, a powerful molecular biological tool, pyrosequencing, was used to test how route of delivery and nutrition altered the piglet ileal microbiota. Multiple important observations were made; several issues were discussed in these studies. The conclusions were important add-ons to current microbiota research, and can have long-lasting impacts on infant nutrition study in the future.","abstract_html":"The human gastrointestinal (GI) tract is colonized by a dense and diverse bacterial community called the commensal microbiota, which plays an important role in overall health of individuals. However, due to the extreme complexity of microbiota composition and limits of research tools, there were many challenges in identify and compare individual members of the microbiota, and the impacts of multiple factors on the development of microbiota were poorly understood. This is a comprehensive study on applying traditional culture dependent methods, as well as the latest developed molecular biological tools, in neonatal piglets’ intestinal microbiome and lactobacilli community structure research. In Chapter 2, the latest progress of methods used in microbiota research were described and compared, the known impact of different environmental and dietary factors were discussed. In Chapter 3, a new protocol of Terminal restriction fragment length polymorphism (T-RFLP) analysis was developed to facilitate lactobacilli composition study. In Chapter 4, both culture dependent methods and the novel T-RFLP tools were used to reveal the impact of piglet age and route of delivery on ileal Lactobacillus diversity. In Chapter 5, a powerful molecular biological tool, pyrosequencing, was used to test how route of delivery and nutrition altered the piglet ileal microbiota. Multiple important observations were made; several issues were discussed in these studies. The conclusions were important add-ons to current microbiota research, and can have long-lasting impacts on infant nutrition study in the future.","abstract_has_math":false,"creators":["Chen, Long"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Food Science & Human Nutrition","degree_department":null,"school":null,"contributors":["Miller, Michael J.","Donovan, Sharon M.","Chassy, Bruce","Jin, Yong-Su"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-02-03T19:37:21Z","date_published":"2013-02-03T19:37:21Z","updated_at":"2026-07-22T22:25:33Z","subjects":["microbiota","microbiome","pyrosequencing","terminal restriction fragment length polymorphism (T-RFLP)","Lactobacilli","gastrointestinal (GI) tract"],"languages":["en"],"rights":["Copyright 2012 Long Chen"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/42397","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Miller, Michael J.","Donovan, Sharon M.","Chassy, Bruce","Jin, Yong-Su"]},{"key":"dc:creator","label":"Author","values":["Chen, Long"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2013-02-03T19:37:21Z","2012-12"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Food Science & Human Nutrition"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["microbiota","microbiome","pyrosequencing","terminal restriction fragment length polymorphism (T-RFLP)","Lactobacilli","gastrointestinal (GI) tract"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2012 Long Chen"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/42397"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The human gastrointestinal (GI) tract is colonized by a dense and diverse bacterial community called the commensal microbiota, which plays an important role in overall health of individuals. However, due to the extreme complexity of microbiota composition and limits of research tools, there were many challenges in identify and compare individual members of the microbiota, and the impacts of multiple factors on the development of microbiota were poorly understood. This is a comprehensive study on applying traditional culture dependent methods, as well as the latest developed molecular biological tools, in neonatal piglets’ intestinal microbiome and lactobacilli community structure research. In Chapter 2, the latest progress of methods used in microbiota research were described and compared, the known impact of different environmental and dietary factors were discussed. In Chapter 3, a new protocol of Terminal restriction fragment length polymorphism (T-RFLP) analysis was developed to facilitate lactobacilli composition study. In Chapter 4, both culture dependent methods and the novel T-RFLP tools were used to reveal the impact of piglet age and route of delivery on ileal Lactobacillus diversity. In Chapter 5, a powerful molecular biological tool, pyrosequencing, was used to test how route of delivery and nutrition altered the piglet ileal microbiota. Multiple important observations were made; several issues were discussed in these studies. The conclusions were important add-ons to current microbiota research, and can have long-lasting impacts on infant nutrition study in the future.","Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2012-12-06T19:44:50Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 CHEN_LONG.pdf: 1739137 bytes, checksum: 345e1c1e792af536cb96abdbcb015a13 (MD5)","Made available in DSpace on 2013-02-03T19:37:21Z (GMT). No. of bitstreams: 2 Long_Chen.pdf: 1718884 bytes, checksum: 057010834e0cb86d1c0bdc125686be13 (MD5) license.txt: 4056 bytes, checksum: 1948b48b24423802036fb31083d6e8ef (MD5)"]},{"key":"dc:title","label":"Title","values":["Characterization of the intestinal microbiome and lactobacilli community: influence of dietary and environmental factors"]}]}],"canonical_facts":{"dc:contributor":["Miller, Michael J.","Donovan, Sharon M.","Chassy, Bruce","Jin, Yong-Su"],"dc:creator":["Chen, Long"],"dc:date":["2013-02-03T19:37:21Z","2012-12"],"dc:description":["The human gastrointestinal (GI) tract is colonized by a dense and diverse bacterial community called the commensal microbiota, which plays an important role in overall health of individuals. However, due to the extreme complexity of microbiota composition and limits of research tools, there were many challenges in identify and compare individual members of the microbiota, and the impacts of multiple factors on the development of microbiota were poorly understood. This is a comprehensive study on applying traditional culture dependent methods, as well as the latest developed molecular biological tools, in neonatal piglets’ intestinal microbiome and lactobacilli community structure research. In Chapter 2, the latest progress of methods used in microbiota research were described and compared, the known impact of different environmental and dietary factors were discussed. In Chapter 3, a new protocol of Terminal restriction fragment length polymorphism (T-RFLP) analysis was developed to facilitate lactobacilli composition study. In Chapter 4, both culture dependent methods and the novel T-RFLP tools were used to reveal the impact of piglet age and route of delivery on ileal Lactobacillus diversity. In Chapter 5, a powerful molecular biological tool, pyrosequencing, was used to test how route of delivery and nutrition altered the piglet ileal microbiota. Multiple important observations were made; several issues were discussed in these studies. The conclusions were important add-ons to current microbiota research, and can have long-lasting impacts on infant nutrition study in the future.","Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2012-12-06T19:44:50Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 CHEN_LONG.pdf: 1739137 bytes, checksum: 345e1c1e792af536cb96abdbcb015a13 (MD5)","Made available in DSpace on 2013-02-03T19:37:21Z (GMT). No. of bitstreams: 2 Long_Chen.pdf: 1718884 bytes, checksum: 057010834e0cb86d1c0bdc125686be13 (MD5) license.txt: 4056 bytes, checksum: 1948b48b24423802036fb31083d6e8ef (MD5)"],"dc:identifier":["http://hdl.handle.net/2142/42397"],"dc:language":["en"],"dc:rights":["Copyright 2012 Long Chen"],"dc:subject":["microbiota","microbiome","pyrosequencing","terminal restriction fragment length polymorphism (T-RFLP)","Lactobacilli","gastrointestinal (GI) tract"],"dc:title":["Characterization of the intestinal microbiome and lactobacilli community: influence of dietary and environmental factors"],"dc:type":["text"],"thesis:degree_discipline":["Food Science & Human Nutrition"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:33Z"}