Roles of surface interaction on Cryptosporidium parvum oocyst transport in subsurface environment
type: CreativeWork
description: The influence of Cryptosporidium (C.) parvum oocyst and collector surface properties on transport of C. parvum oocysts and microspheres was studied with a radial stagnation point flow (RSPF) cell and 2-dimensional (2-D) micromodels. The quartz or natural organic matter coated quartz surfaces were used as collector in the RSPF cell. The cylindrical silica collectors or silica collectors coated with 0, 10, 20, 50 and, 100% Fe2O3 patches were firstly fabricated in micromodels. Real time transport of oocyst in RSPF cell and micromodel in electrolyte solutions with systematically varied composition was monitored. Surface polarity and chemical heterogeneity of oocysts surface were characterized by liquid chromatography/nanoelectrospray ionization tandem mass spectrometry (LC-MS/MS), microbial adhesion to hydrocarbon test (MATH), contact angle, and Fourier transform Infrared Spectroscopic (FT-IR) imaging. The most important fundings of this research are the followings: 1) this research developed a method to precisely design complicated yet well-controlled collector properties of porous media and showed the importance of spatial distribution of charged heterogeneous patches on collectors; 2) the results illustrated that the composition and conformation of oocyst surface macromolecules determined oocyst transport by controlling the surface interactions and complexations between oocyst and quartz, NOM, Fe2O3 and charged heterogeneous mineral surfaces. Especially, this research firstly revealed that the electrophoretic softness of oocyst surface macromolecules was essential to van der Waals interaction and steric repulsion between oocyst and quartz surfaces. RSPF experiments illustrated that steric repulsion caused by oocyst surface macromolecules prevented oocyst deposition at higher ionic strength where the Derjaguin–Landau–Verwey–Overbeek (DLVO) theory predicted maximum deposition. Charge heterogeneity was responsible for oocyst deposition on natural organic matter (NOM) su…
abstract: The influence of Cryptosporidium (C.) parvum oocyst and collector surface properties on transport of C. parvum oocysts and microspheres was studied with a radial stagnation point flow (RSPF) cell and 2-dimensional (2-D) micromodels. The quartz or natural organic matter coated quartz surfaces were used as collector in the RSPF cell. The cylindrical silica collectors or silica collectors coated with 0, 10, 20, 50 and, 100% Fe2O3 patches were firstly fabricated in micromodels. Real time transport of oocyst in RSPF cell and micromodel in electrolyte solutions with systematically varied composition was monitored. Surface polarity and chemical heterogeneity of oocysts surface were characterized by liquid chromatography/nanoelectrospray ionization tandem mass spectrometry (LC-MS/MS), microbial adhesion to hydrocarbon test (MATH), contact angle, and Fourier transform Infrared Spectroscopic (FT-IR) imaging. The most important fundings of this research are the followings: 1) this research developed a method to precisely design complicated yet well-controlled collector properties of porous media and showed the importance of spatial distribution of charged heterogeneous patches on collectors; 2) the results illustrated that the composition and conformation of oocyst surface macromolecules determined oocyst transport by controlling the surface interactions and complexations between oocyst and quartz, NOM, Fe2O3 and charged heterogeneous mineral surfaces. Especially, this research firstly revealed that the electrophoretic softness of oocyst surface macromolecules was essential to van der Waals interaction and steric repulsion between oocyst and quartz surfaces. RSPF experiments illustrated that steric repulsion caused by oocyst surface macromolecules prevented oocyst deposition at higher ionic strength where the Derjaguin–Landau–Verwey–Overbeek (DLVO) theory predicted maximum deposition. Charge heterogeneity was responsible for oocyst deposition on natural organic matter (NOM) su…
author: Liu, Yuanyuan
language: en
keywords: Cryptosporidium parvum oocysts, transport, porous media, micromodel, charge heterogeneity
date published: 2013-02-03T19:31:06Z
date modified: 2026-07-22T22:25:33Z
provider: University of Illinois - Urbana-Champaign
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Illinois at Urbana-Champaign

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2142/42310