{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/31131"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/31131","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Induction of epithelial to mesenchymal transition in mesothelial cells by secreted factors from uterine epithelial cells","abstract":"Made available in DSpace on 2012-05-22T00:30:00Z (GMT). No. of bitstreams: 2 Masoud_Farzaneh.pdf: 34313918 bytes, checksum: e99d6869329b04b746cc82321b8f70bc (MD5) license.txt: 4065 bytes, checksum: 032671e283e8d231a619ff8c90294786 (MD5)","abstract_html":"Made available in DSpace on 2012-05-22T00:30:00Z (GMT). No. of bitstreams: 2 Masoud_Farzaneh.pdf: 34313918 bytes, checksum: e99d6869329b04b746cc82321b8f70bc (MD5) license.txt: 4065 bytes, checksum: 032671e283e8d231a619ff8c90294786 (MD5)","abstract_has_math":false,"creators":["Masoud, Farzaneh"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Cell and Developmental Biology","degree_department":null,"school":null,"contributors":["Nowak, Romana A.","Bellini, Michel","Cameron, Jo Ann","Ceman, Stephanie S.","Kemper, Byron W."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2012,"date_issued":"2012-05-22T00:30:00Z","date_published":"2012-05-22T00:30:00Z","updated_at":"2026-07-22T22:25:30Z","subjects":["epithelial to mesenchymal transition (EMT)","Mesothelial cells","Cytokines","Human Uterine Epithelial Cell Culture (HES)","extracellular matrix metalloproteinase inducer (EMMPRIN)"],"languages":["en"],"rights":["Copyright 2012 Farzaneh Masoud"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/31131","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Nowak, Romana A.","Bellini, Michel","Cameron, Jo Ann","Ceman, Stephanie S.","Kemper, Byron W."]},{"key":"dc:creator","label":"Author","values":["Masoud, Farzaneh"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2012-05-22T00:30:00Z","2012-05"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Cell and Developmental Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["epithelial to mesenchymal transition (EMT)","Mesothelial cells","Cytokines","Human Uterine Epithelial Cell Culture (HES)","extracellular matrix metalloproteinase inducer (EMMPRIN)"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2012 Farzaneh Masoud"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/31131"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Made available in DSpace on 2012-05-22T00:30:00Z (GMT). No. of bitstreams: 2 Masoud_Farzaneh.pdf: 34313918 bytes, checksum: e99d6869329b04b746cc82321b8f70bc (MD5) license.txt: 4065 bytes, checksum: 032671e283e8d231a619ff8c90294786 (MD5)","Establishment of the disease endometriosis requires the attachment and invasion of endometrial fragments into the peritoneal lining of the peritoneal cavity. For proper attachment and invasion to occur, the endometrial cells and mesothelial cells that line the surface of the peritoneum must interact. Previous studies by Witz et al. have shown that endometrial fragments can attach to mesothelial cells, but the mechanism by which these endometrial cells are able to invade through the mesothelial layer into the underlying peritoneum is not clear (Witz et al 1999, 2000, 2001). Mesothelial cells normally display an epithelial–like morphology, but in response to injury or inflammation they undergo epithelial to mesenchymal transition (EMT). As mesothelial cells undergo EMT, they lose expression of E-cadherin and cytokeratins, exhibit a fibroblast-like phenotype, and become more migratory and invasive. This transition results in the formation of gaps in the mesothelium and could allow attached endometrial cells to invade into the underlying peritoneum. Recent studies have reported the up-regulation of extracellular matrix metalloproteinase inducer, (EMMPRIN) as cancer cells undergo EMT (Abraham et al 2008). The goal of my research proposal is to determine whether secreted factors from uterine epithelial cells of endometrial fragments can cause epithelial to mesenchymal transition of mesothelial cells. In this study we utilized a human mesothelial cell line, LP-9, to examine the effect of EMMPRIN, and other uterine epithelial cell factors such as IL-1β, and TGF-β1 on EMT by monitoring changes in expression of N-cadherin, MMPs, cytokeratins, vimentin, and transcription factors twist and snail. Change in rate of migration was also assessed using the in vitro cell wounding assay.","Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2012-03-16T18:19:24Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 3 Masoud_Farzaneh.docx: 33437610 bytes, checksum: 3a6270776fe617281a411ab04b555489 (MD5) Masoud_Farzaneh.pdf: 34397748 bytes, checksum: 3ebc605a18ffa3144a21486e12792264 (MD5) Masoud_Farzaneh.pdf: 34397748 bytes, checksum: 3ebc605a18ffa3144a21486e12792264 (MD5)"]},{"key":"dc:title","label":"Title","values":["Induction of epithelial to mesenchymal transition in mesothelial cells by secreted factors from uterine epithelial cells"]}]}],"canonical_facts":{"dc:contributor":["Nowak, Romana A.","Bellini, Michel","Cameron, Jo Ann","Ceman, Stephanie S.","Kemper, Byron W."],"dc:creator":["Masoud, Farzaneh"],"dc:date":["2012-05-22T00:30:00Z","2012-05"],"dc:description":["Made available in DSpace on 2012-05-22T00:30:00Z (GMT). No. of bitstreams: 2 Masoud_Farzaneh.pdf: 34313918 bytes, checksum: e99d6869329b04b746cc82321b8f70bc (MD5) license.txt: 4065 bytes, checksum: 032671e283e8d231a619ff8c90294786 (MD5)","Establishment of the disease endometriosis requires the attachment and invasion of endometrial fragments into the peritoneal lining of the peritoneal cavity. For proper attachment and invasion to occur, the endometrial cells and mesothelial cells that line the surface of the peritoneum must interact. Previous studies by Witz et al. have shown that endometrial fragments can attach to mesothelial cells, but the mechanism by which these endometrial cells are able to invade through the mesothelial layer into the underlying peritoneum is not clear (Witz et al 1999, 2000, 2001). Mesothelial cells normally display an epithelial–like morphology, but in response to injury or inflammation they undergo epithelial to mesenchymal transition (EMT). As mesothelial cells undergo EMT, they lose expression of E-cadherin and cytokeratins, exhibit a fibroblast-like phenotype, and become more migratory and invasive. This transition results in the formation of gaps in the mesothelium and could allow attached endometrial cells to invade into the underlying peritoneum. Recent studies have reported the up-regulation of extracellular matrix metalloproteinase inducer, (EMMPRIN) as cancer cells undergo EMT (Abraham et al 2008). The goal of my research proposal is to determine whether secreted factors from uterine epithelial cells of endometrial fragments can cause epithelial to mesenchymal transition of mesothelial cells. In this study we utilized a human mesothelial cell line, LP-9, to examine the effect of EMMPRIN, and other uterine epithelial cell factors such as IL-1β, and TGF-β1 on EMT by monitoring changes in expression of N-cadherin, MMPs, cytokeratins, vimentin, and transcription factors twist and snail. Change in rate of migration was also assessed using the in vitro cell wounding assay.","Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2012-03-16T18:19:24Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 3 Masoud_Farzaneh.docx: 33437610 bytes, checksum: 3a6270776fe617281a411ab04b555489 (MD5) Masoud_Farzaneh.pdf: 34397748 bytes, checksum: 3ebc605a18ffa3144a21486e12792264 (MD5) Masoud_Farzaneh.pdf: 34397748 bytes, checksum: 3ebc605a18ffa3144a21486e12792264 (MD5)"],"dc:identifier":["http://hdl.handle.net/2142/31131"],"dc:language":["en"],"dc:rights":["Copyright 2012 Farzaneh Masoud"],"dc:subject":["epithelial to mesenchymal transition (EMT)","Mesothelial cells","Cytokines","Human Uterine Epithelial Cell Culture (HES)","extracellular matrix metalloproteinase inducer (EMMPRIN)"],"dc:title":["Induction of epithelial to mesenchymal transition in mesothelial cells by secreted factors from uterine epithelial cells"],"dc:type":["text"],"thesis:degree_discipline":["Cell and Developmental Biology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:30Z"}