{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/31058"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/31058","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Evolution of the Gr family of gustatory and odorant receptors in mosquitoes","abstract":"The ability to detect chemical information from the environment is of critical importance to insects, whose ecological and evolutionary boundaries typically revolve around taste and smell. Of the receptors involved in chemoperception, the gustatory receptor (Gr) protein family comprises most of the diversity in the insect chemoreceptor superfamily, including within it not only taste receptors, but select olfactory receptors as well. Manual annotation of the Gr family in the genome sequence of the yellow fever mosquito, Aedes aegypti Linnaeus, yielded a total of 114 potential proteins encoded by 79 genes. In the sequenced genome, 23 of these genes and protein isoforms are pseudogenic, leaving 91 putatively functional Grs. Comparison with the set of 76 Grs encoded by 52 genes in the distantly related Anopheles gambiae Giles mosquito revealed 13 new AgGrs encoded by eight genes. Phylogenetic analysis reveals the conservation of carbon dioxide, sugar and several orphan receptors in these two mosquitoes and Drosophila flies. Despite these similarities, most of these Grs are unique to mosquitoes and many are specific to the Aedes or Anopheles lineages, indicating their involvement in mosquito-specific aspects of both gustatory and olfactory perception. In particular, most instances of alternative splicing in orthologous loci appear to have evolved after the culicine-anopheline split ±150 million years ago. Within the Gr family, it has been shown that Gr21a and Gr63 in Drosophila melanogaster Meigen combine to form a functional heterodimer for the perception of carbon dioxide. These genes are highly conserved, along with a paralog of Gr21a, in mosquitoes. We examined the presence of these genes in other insect lineages, and although highly conserved in some lineages (three mosquito species, silk moth and red flour beetle), the data suggest that arthropods likely evolved multiple strategies to confer recognition of carbon dioxide. The forthcoming mosquito Gr gene models will help serve as a necessary starting place for further research, as we expect the continued deorphanization of receptors to lead to novel methods for repelling pests and consequently slowing the spread of vector-borne disease.","abstract_html":"The ability to detect chemical information from the environment is of critical importance to insects, whose ecological and evolutionary boundaries typically revolve around taste and smell. Of the receptors involved in chemoperception, the gustatory receptor (Gr) protein family comprises most of the diversity in the insect chemoreceptor superfamily, including within it not only taste receptors, but select olfactory receptors as well. Manual annotation of the Gr family in the genome sequence of the yellow fever mosquito, Aedes aegypti Linnaeus, yielded a total of 114 potential proteins encoded by 79 genes. In the sequenced genome, 23 of these genes and protein isoforms are pseudogenic, leaving 91 putatively functional Grs. Comparison with the set of 76 Grs encoded by 52 genes in the distantly related Anopheles gambiae Giles mosquito revealed 13 new AgGrs encoded by eight genes. Phylogenetic analysis reveals the conservation of carbon dioxide, sugar and several orphan receptors in these two mosquitoes and Drosophila flies. Despite these similarities, most of these Grs are unique to mosquitoes and many are specific to the Aedes or Anopheles lineages, indicating their involvement in mosquito-specific aspects of both gustatory and olfactory perception. In particular, most instances of alternative splicing in orthologous loci appear to have evolved after the culicine-anopheline split ±150 million years ago. Within the Gr family, it has been shown that Gr21a and Gr63 in Drosophila melanogaster Meigen combine to form a functional heterodimer for the perception of carbon dioxide. These genes are highly conserved, along with a paralog of Gr21a, in mosquitoes. We examined the presence of these genes in other insect lineages, and although highly conserved in some lineages (three mosquito species, silk moth and red flour beetle), the data suggest that arthropods likely evolved multiple strategies to confer recognition of carbon dioxide. The forthcoming mosquito Gr gene models will help serve as a necessary starting place for further research, as we expect the continued deorphanization of receptors to lead to novel methods for repelling pests and consequently slowing the spread of vector-borne disease.","abstract_has_math":false,"creators":["Kent, Lauren"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Entomology","degree_department":null,"school":null,"contributors":["Robertson, Hugh M.","Berlocher, Stewart H.","Berenbaum, May R.","Hanks, Lawrence M.","Nardi, James B."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2012,"date_issued":"2012-05-22T00:25:02Z","date_published":"2012-05-22T00:25:02Z","updated_at":"2026-07-22T22:25:29Z","subjects":["chemoreceptors","molecular evolution","gustatory receptors (Grs)","mosquito taste"],"languages":["en"],"rights":["Copyright 2012 Lauren Bettine Kent"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/31058","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Robertson, Hugh M.","Berlocher, Stewart H.","Berenbaum, May R.","Hanks, Lawrence M.","Nardi, James B."]},{"key":"dc:creator","label":"Author","values":["Kent, Lauren"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2012-05-22T00:25:02Z","2012-05"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Entomology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["chemoreceptors","molecular evolution","gustatory receptors (Grs)","mosquito taste"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2012 Lauren Bettine Kent"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/31058"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The ability to detect chemical information from the environment is of critical importance to insects, whose ecological and evolutionary boundaries typically revolve around taste and smell. Of the receptors involved in chemoperception, the gustatory receptor (Gr) protein family comprises most of the diversity in the insect chemoreceptor superfamily, including within it not only taste receptors, but select olfactory receptors as well. Manual annotation of the Gr family in the genome sequence of the yellow fever mosquito, Aedes aegypti Linnaeus, yielded a total of 114 potential proteins encoded by 79 genes. In the sequenced genome, 23 of these genes and protein isoforms are pseudogenic, leaving 91 putatively functional Grs. Comparison with the set of 76 Grs encoded by 52 genes in the distantly related Anopheles gambiae Giles mosquito revealed 13 new AgGrs encoded by eight genes. Phylogenetic analysis reveals the conservation of carbon dioxide, sugar and several orphan receptors in these two mosquitoes and Drosophila flies. Despite these similarities, most of these Grs are unique to mosquitoes and many are specific to the Aedes or Anopheles lineages, indicating their involvement in mosquito-specific aspects of both gustatory and olfactory perception. In particular, most instances of alternative splicing in orthologous loci appear to have evolved after the culicine-anopheline split ±150 million years ago. Within the Gr family, it has been shown that Gr21a and Gr63 in Drosophila melanogaster Meigen combine to form a functional heterodimer for the perception of carbon dioxide. These genes are highly conserved, along with a paralog of Gr21a, in mosquitoes. We examined the presence of these genes in other insect lineages, and although highly conserved in some lineages (three mosquito species, silk moth and red flour beetle), the data suggest that arthropods likely evolved multiple strategies to confer recognition of carbon dioxide. The forthcoming mosquito Gr gene models will help serve as a necessary starting place for further research, as we expect the continued deorphanization of receptors to lead to novel methods for repelling pests and consequently slowing the spread of vector-borne disease.","Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2012-04-20T17:10:22Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 10 References.doc: 85504 bytes, checksum: 7b0d7fe5a5abc1e96cf83debb60d67bf (MD5) FIGURES AND TABLES.doc: 4712960 bytes, checksum: 0308a410b95a12aa50c366e4657f71e5 (MD5) chapters.doc: 111616 bytes, checksum: ddd859d25707fa148613e365ee009aca (MD5) table of contents.doc: 22528 bytes, checksum: 25853022237e3f93e6fe11ecd76b504a (MD5) Acknowledgements.doc: 24576 bytes, checksum: c361de5bd1f297754b16cc4d1bb8264f (MD5) dedication.doc: 19968 bytes, checksum: 56a2d09ec234e4f7e98ec023b312ba30 (MD5) Abstract.doc: 24064 bytes, checksum: e1644d2e5416b387fa42c0d6557ea84f (MD5) TitlePage.doc: 27648 bytes, checksum: 4a0d00029d4d9efd1ee1599699dd9992 (MD5) copyright.doc: 19456 bytes, checksum: 021137c755044dff845c6ac270ed2081 (MD5) Kent_Lauren.pdf: 4483425 bytes, checksum: fad96eded351b77ece70443fd2c5a6d2 (MD5)","Made available in DSpace on 2012-05-22T00:25:02Z (GMT). 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Of the receptors involved in chemoperception, the gustatory receptor (Gr) protein family comprises most of the diversity in the insect chemoreceptor superfamily, including within it not only taste receptors, but select olfactory receptors as well. Manual annotation of the Gr family in the genome sequence of the yellow fever mosquito, Aedes aegypti Linnaeus, yielded a total of 114 potential proteins encoded by 79 genes. In the sequenced genome, 23 of these genes and protein isoforms are pseudogenic, leaving 91 putatively functional Grs. Comparison with the set of 76 Grs encoded by 52 genes in the distantly related Anopheles gambiae Giles mosquito revealed 13 new AgGrs encoded by eight genes. Phylogenetic analysis reveals the conservation of carbon dioxide, sugar and several orphan receptors in these two mosquitoes and Drosophila flies. Despite these similarities, most of these Grs are unique to mosquitoes and many are specific to the Aedes or Anopheles lineages, indicating their involvement in mosquito-specific aspects of both gustatory and olfactory perception. In particular, most instances of alternative splicing in orthologous loci appear to have evolved after the culicine-anopheline split ±150 million years ago. Within the Gr family, it has been shown that Gr21a and Gr63 in Drosophila melanogaster Meigen combine to form a functional heterodimer for the perception of carbon dioxide. These genes are highly conserved, along with a paralog of Gr21a, in mosquitoes. We examined the presence of these genes in other insect lineages, and although highly conserved in some lineages (three mosquito species, silk moth and red flour beetle), the data suggest that arthropods likely evolved multiple strategies to confer recognition of carbon dioxide. The forthcoming mosquito Gr gene models will help serve as a necessary starting place for further research, as we expect the continued deorphanization of receptors to lead to novel methods for repelling pests and consequently slowing the spread of vector-borne disease.","Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2012-04-20T17:10:22Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 10 References.doc: 85504 bytes, checksum: 7b0d7fe5a5abc1e96cf83debb60d67bf (MD5) FIGURES AND TABLES.doc: 4712960 bytes, checksum: 0308a410b95a12aa50c366e4657f71e5 (MD5) chapters.doc: 111616 bytes, checksum: ddd859d25707fa148613e365ee009aca (MD5) table of contents.doc: 22528 bytes, checksum: 25853022237e3f93e6fe11ecd76b504a (MD5) Acknowledgements.doc: 24576 bytes, checksum: c361de5bd1f297754b16cc4d1bb8264f (MD5) dedication.doc: 19968 bytes, checksum: 56a2d09ec234e4f7e98ec023b312ba30 (MD5) Abstract.doc: 24064 bytes, checksum: e1644d2e5416b387fa42c0d6557ea84f (MD5) TitlePage.doc: 27648 bytes, checksum: 4a0d00029d4d9efd1ee1599699dd9992 (MD5) copyright.doc: 19456 bytes, checksum: 021137c755044dff845c6ac270ed2081 (MD5) Kent_Lauren.pdf: 4483425 bytes, checksum: fad96eded351b77ece70443fd2c5a6d2 (MD5)","Made available in DSpace on 2012-05-22T00:25:02Z (GMT). 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