{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/31042"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/31042","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Nutritional and immunological outcomes as affected by a novel carbohydrate complex composed of galactoglucomannan oligosaccharides and arabinoxylan","abstract":"The objective of this research was to evaluate a novel galactoglucomannan oligosaccharide-arabinoxylan (GGMO-AX) complex for properties that could positively impact nutritional and immunological outcomes. Five studies were designed to address three major research objectives: 1) determine the structural and chemical composition of the GGMO-AX substrate and select fractions, 2) determine the hydrolytic digestibility and fermentative capacity of the GGMO-AX substrate in vitro and in vivo, and 3) determine the immunological effects of GGMO-AX in a pathogen-challenged avian model. Study 1 evaluated the structural composition of the GGMO-AX components as determined by a combination of limited hydrolysis, monosaccharide composition and linkage analysis, size exclusion fractionation and MALDITOF/ MS analysis of component GGMO, and 1D and 2D NMR techniques. Study 2 evaluated the hydrolytic digestibility, fermentative capacity, and microbiota modulating properties of GGMO-AX and four fractions of GGMO-AX. Study 3 evaluated nutritional effects and prebiotic potential of spray-dried GGMO-AX when added to canine diets and tested in a doseresponse experiment. Studies 4 and 5 determined the effects of supplemental GGMO-AX in diets with emphasis on growth performance, fermentative effects, and immune indices in an avian model challenged with an acute coccidial (Eimeria acervulina; EA) or Salmonella typhimurium (ST) infection. Results indicated that GGMOs have a degree of polymerization (DP) from 4 to 13, with the major component being DP 5-8. The structure of these oligosaccharides is a β-1,4-linked backbone of Man and Glc residues, with occasional α-1,6 branching by single galactosyl units. The GGMO-AX substrate is resistant to hydrolytic digestion, well-fermented, and positively modulates microbial populations as measured in vitro and in vivo. When chicks were challenged with EA, a strain of avian coccidiosis, and iii supplemented with select concentrations of GGMO-AX, chick performance was decreased, but GGMO-AX supplementation improved select fermentation indices and the innate intestinal immune response. During a ST infection, GGMO-AX elicited a prebiotic effect and appeared to decrease the virulence of the ST within the digestive tract, but did not limit ST intestinal colonization or shedding. Overall, GGMO-AX appears to be well fermented in vitro and in vivo and able to elicit a prebiotic effect in select animal models. Dietary GGMO-AX supplementation is able to improve the innate immune response to an EA infection and potentially decrease ST virulence.","abstract_html":"The objective of this research was to evaluate a novel galactoglucomannan oligosaccharide-arabinoxylan (GGMO-AX) complex for properties that could positively impact nutritional and immunological outcomes. Five studies were designed to address three major research objectives: 1) determine the structural and chemical composition of the GGMO-AX substrate and select fractions, 2) determine the hydrolytic digestibility and fermentative capacity of the GGMO-AX substrate in vitro and in vivo, and 3) determine the immunological effects of GGMO-AX in a pathogen-challenged avian model. Study 1 evaluated the structural composition of the GGMO-AX components as determined by a combination of limited hydrolysis, monosaccharide composition and linkage analysis, size exclusion fractionation and MALDITOF/ MS analysis of component GGMO, and 1D and 2D NMR techniques. Study 2 evaluated the hydrolytic digestibility, fermentative capacity, and microbiota modulating properties of GGMO-AX and four fractions of GGMO-AX. Study 3 evaluated nutritional effects and prebiotic potential of spray-dried GGMO-AX when added to canine diets and tested in a doseresponse experiment. Studies 4 and 5 determined the effects of supplemental GGMO-AX in diets with emphasis on growth performance, fermentative effects, and immune indices in an avian model challenged with an acute coccidial (Eimeria acervulina; EA) or Salmonella typhimurium (ST) infection. Results indicated that GGMOs have a degree of polymerization (DP) from 4 to 13, with the major component being DP 5-8. The structure of these oligosaccharides is a β-1,4-linked backbone of Man and Glc residues, with occasional α-1,6 branching by single galactosyl units. The GGMO-AX substrate is resistant to hydrolytic digestion, well-fermented, and positively modulates microbial populations as measured in vitro and in vivo. When chicks were challenged with EA, a strain of avian coccidiosis, and iii supplemented with select concentrations of GGMO-AX, chick performance was decreased, but GGMO-AX supplementation improved select fermentation indices and the innate intestinal immune response. During a ST infection, GGMO-AX elicited a prebiotic effect and appeared to decrease the virulence of the ST within the digestive tract, but did not limit ST intestinal colonization or shedding. Overall, GGMO-AX appears to be well fermented in vitro and in vivo and able to elicit a prebiotic effect in select animal models. Dietary GGMO-AX supplementation is able to improve the innate immune response to an EA infection and potentially decrease ST virulence.","abstract_has_math":false,"creators":["Faber, Trevor"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Animal Sciences","degree_department":null,"school":null,"contributors":["Fahey, George C.","Dilger, Ryan N.","Parsons, Carl M.","Tappenden, Kelly A."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2012,"date_issued":"2012-05-22T00:23:58Z","date_published":"2012-05-22T00:23:58Z","updated_at":"2026-07-22T22:25:29Z","subjects":["Galactoglucomannan oligosaccharide","Dietary Fiber","Eimeria acervulina","Salmonella typhimurium","In vitro fermentation","Prebiotic"],"languages":["en"],"rights":["Copyright 2012 Trevor Austin Faber"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/31042","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Fahey, George C.","Dilger, Ryan N.","Parsons, Carl M.","Tappenden, Kelly A."]},{"key":"dc:creator","label":"Author","values":["Faber, Trevor"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2012-05-22T00:23:58Z","2012-05"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Animal Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Galactoglucomannan oligosaccharide","Dietary Fiber","Eimeria acervulina","Salmonella typhimurium","In vitro fermentation","Prebiotic"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2012 Trevor Austin Faber"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/31042"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The objective of this research was to evaluate a novel galactoglucomannan oligosaccharide-arabinoxylan (GGMO-AX) complex for properties that could positively impact nutritional and immunological outcomes. Five studies were designed to address three major research objectives: 1) determine the structural and chemical composition of the GGMO-AX substrate and select fractions, 2) determine the hydrolytic digestibility and fermentative capacity of the GGMO-AX substrate in vitro and in vivo, and 3) determine the immunological effects of GGMO-AX in a pathogen-challenged avian model. Study 1 evaluated the structural composition of the GGMO-AX components as determined by a combination of limited hydrolysis, monosaccharide composition and linkage analysis, size exclusion fractionation and MALDITOF/ MS analysis of component GGMO, and 1D and 2D NMR techniques. Study 2 evaluated the hydrolytic digestibility, fermentative capacity, and microbiota modulating properties of GGMO-AX and four fractions of GGMO-AX. Study 3 evaluated nutritional effects and prebiotic potential of spray-dried GGMO-AX when added to canine diets and tested in a doseresponse experiment. Studies 4 and 5 determined the effects of supplemental GGMO-AX in diets with emphasis on growth performance, fermentative effects, and immune indices in an avian model challenged with an acute coccidial (Eimeria acervulina; EA) or Salmonella typhimurium (ST) infection. Results indicated that GGMOs have a degree of polymerization (DP) from 4 to 13, with the major component being DP 5-8. The structure of these oligosaccharides is a β-1,4-linked backbone of Man and Glc residues, with occasional α-1,6 branching by single galactosyl units. The GGMO-AX substrate is resistant to hydrolytic digestion, well-fermented, and positively modulates microbial populations as measured in vitro and in vivo. When chicks were challenged with EA, a strain of avian coccidiosis, and iii supplemented with select concentrations of GGMO-AX, chick performance was decreased, but GGMO-AX supplementation improved select fermentation indices and the innate intestinal immune response. During a ST infection, GGMO-AX elicited a prebiotic effect and appeared to decrease the virulence of the ST within the digestive tract, but did not limit ST intestinal colonization or shedding. Overall, GGMO-AX appears to be well fermented in vitro and in vivo and able to elicit a prebiotic effect in select animal models. Dietary GGMO-AX supplementation is able to improve the innate immune response to an EA infection and potentially decrease ST virulence.","Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2012-01-04T14:53:37Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Faber_Trevor.docx: 1825209 bytes, checksum: f4d994b2cd08ead6dcb7effe649893cb (MD5) Faber_Trevor.pdf: 1460654 bytes, checksum: cdb5dea09028670cc4139724801a88b2 (MD5)","Made available in DSpace on 2012-05-22T00:23:58Z (GMT). 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Five studies were designed to address three major research objectives: 1) determine the structural and chemical composition of the GGMO-AX substrate and select fractions, 2) determine the hydrolytic digestibility and fermentative capacity of the GGMO-AX substrate in vitro and in vivo, and 3) determine the immunological effects of GGMO-AX in a pathogen-challenged avian model. Study 1 evaluated the structural composition of the GGMO-AX components as determined by a combination of limited hydrolysis, monosaccharide composition and linkage analysis, size exclusion fractionation and MALDITOF/ MS analysis of component GGMO, and 1D and 2D NMR techniques. Study 2 evaluated the hydrolytic digestibility, fermentative capacity, and microbiota modulating properties of GGMO-AX and four fractions of GGMO-AX. Study 3 evaluated nutritional effects and prebiotic potential of spray-dried GGMO-AX when added to canine diets and tested in a doseresponse experiment. Studies 4 and 5 determined the effects of supplemental GGMO-AX in diets with emphasis on growth performance, fermentative effects, and immune indices in an avian model challenged with an acute coccidial (Eimeria acervulina; EA) or Salmonella typhimurium (ST) infection. Results indicated that GGMOs have a degree of polymerization (DP) from 4 to 13, with the major component being DP 5-8. The structure of these oligosaccharides is a β-1,4-linked backbone of Man and Glc residues, with occasional α-1,6 branching by single galactosyl units. The GGMO-AX substrate is resistant to hydrolytic digestion, well-fermented, and positively modulates microbial populations as measured in vitro and in vivo. When chicks were challenged with EA, a strain of avian coccidiosis, and iii supplemented with select concentrations of GGMO-AX, chick performance was decreased, but GGMO-AX supplementation improved select fermentation indices and the innate intestinal immune response. During a ST infection, GGMO-AX elicited a prebiotic effect and appeared to decrease the virulence of the ST within the digestive tract, but did not limit ST intestinal colonization or shedding. Overall, GGMO-AX appears to be well fermented in vitro and in vivo and able to elicit a prebiotic effect in select animal models. Dietary GGMO-AX supplementation is able to improve the innate immune response to an EA infection and potentially decrease ST virulence.","Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2012-01-04T14:53:37Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Faber_Trevor.docx: 1825209 bytes, checksum: f4d994b2cd08ead6dcb7effe649893cb (MD5) Faber_Trevor.pdf: 1460654 bytes, checksum: cdb5dea09028670cc4139724801a88b2 (MD5)","Made available in DSpace on 2012-05-22T00:23:58Z (GMT). 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