{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/29545"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/29545","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Biosynthesis of the antimicrobial peptide epilancin 15X","abstract":"Lantibiotics are polycyclic antimicrobial peptides that are ribosomally synthesized and posttranslationally modified to their biologically active forms. The recently discovered lantibiotic epilancin 15X produced by Staphylococcus epidermidis 15X154 is active against several pathogenic bacteria, including methicillin-resistant S. aureus and vancomycin-resistant Enterococci. Epilancin 15X contains an unusual N-terminal lactate group (Lac) that could be important for its bioactivity. To understand its biosynthesis, the epilancin 15X gene cluster was identified. The Lac group is introduced by dehydration of a Ser residue in the first position of the core peptide by a lantibiotic dehydratase (ElxB), followed by proteolytic removal of the leader peptide by a serine-type protease (ElxP), and hydrolysis of the resulting N-terminal dehydroalanine residue. A pyruvyl group thus formed is finally reduced to Lac by a dehydrogenase (ElxO). Using substrate analogs synthesized by solid-phase peptide synthesis or heterologously expressed in Escherichia coli, the enzymatic activities of ElxO and ElxP and their substrate specificities were investigated in vitro. Furthermore, the dehydratase activity of ElxB was demonstrated in vivo. ElxO tolerates several structural modifications along the substrate sequence and can catalyze the NADPH-dependent reduction of diverse pyruvyl- and oxobutyryl-containing peptides, such as the lantibiotic lactocin S, to the corresponding alcohols. The reaction mechanism of ElxO was proposed based on its x ray crystal structure and a mutagenesis study. The characterization of ElxP demonstrated that the protease can cleave the unmodified precursor peptide and relies on the C-terminal residues of the leader region for substrate recognition. Finally, the stereochemical configuration and the importance of the N-terminal Lac for peptide stability against bacterial aminopeptidases were determined. Understanding the biosynthetic route to epilancin 15X opens the door for engineering of novel lantibiotics with more potent biological activities or different spectra of action.","abstract_html":"Lantibiotics are polycyclic antimicrobial peptides that are ribosomally synthesized and posttranslationally modified to their biologically active forms. The recently discovered lantibiotic epilancin 15X produced by Staphylococcus epidermidis 15X154 is active against several pathogenic bacteria, including methicillin-resistant S. aureus and vancomycin-resistant Enterococci. Epilancin 15X contains an unusual N-terminal lactate group (Lac) that could be important for its bioactivity. To understand its biosynthesis, the epilancin 15X gene cluster was identified. The Lac group is introduced by dehydration of a Ser residue in the first position of the core peptide by a lantibiotic dehydratase (ElxB), followed by proteolytic removal of the leader peptide by a serine-type protease (ElxP), and hydrolysis of the resulting N-terminal dehydroalanine residue. A pyruvyl group thus formed is finally reduced to Lac by a dehydrogenase (ElxO). Using substrate analogs synthesized by solid-phase peptide synthesis or heterologously expressed in Escherichia coli, the enzymatic activities of ElxO and ElxP and their substrate specificities were investigated in vitro. Furthermore, the dehydratase activity of ElxB was demonstrated in vivo. ElxO tolerates several structural modifications along the substrate sequence and can catalyze the NADPH-dependent reduction of diverse pyruvyl- and oxobutyryl-containing peptides, such as the lantibiotic lactocin S, to the corresponding alcohols. The reaction mechanism of ElxO was proposed based on its x ray crystal structure and a mutagenesis study. The characterization of ElxP demonstrated that the protease can cleave the unmodified precursor peptide and relies on the C-terminal residues of the leader region for substrate recognition. Finally, the stereochemical configuration and the importance of the N-terminal Lac for peptide stability against bacterial aminopeptidases were determined. Understanding the biosynthetic route to epilancin 15X opens the door for engineering of novel lantibiotics with more potent biological activities or different spectra of action.","abstract_has_math":false,"creators":["Velasquez, Juan"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["van der Donk, Wilfred A.","Metcalf, William W.","Oldfield, Eric","Silverman, Scott K."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2012,"date_issued":"2012-02-01T00:54:48Z","date_published":"2012-02-01T00:54:48Z","updated_at":"2026-07-22T22:25:27Z","subjects":["Antibiotics","Lantibiotics","Epilancin 15X","Nisin","Antimicrobial peptides","Biosynthesis","Natural Products","Short-Chain Dehydrogenase/Reductase","Serine proteases","Phosphonates"],"languages":["en"],"rights":["Copyright 2011 Juan Esteban Velásquez"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/29545","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["van der Donk, Wilfred A.","Metcalf, William W.","Oldfield, Eric","Silverman, Scott K."]},{"key":"dc:creator","label":"Author","values":["Velasquez, Juan"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2012-02-01T00:54:48Z","2014-02-01T11:00:23Z","2011-12"]},{"key":"dc:type","label":"Dc Type","values":["Dissertation / Thesis","text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Antibiotics","Lantibiotics","Epilancin 15X","Nisin","Antimicrobial peptides","Biosynthesis","Natural Products","Short-Chain Dehydrogenase/Reductase","Serine proteases","Phosphonates"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2011 Juan Esteban Velásquez"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/29545"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Lantibiotics are polycyclic antimicrobial peptides that are ribosomally synthesized and posttranslationally modified to their biologically active forms. The recently discovered lantibiotic epilancin 15X produced by Staphylococcus epidermidis 15X154 is active against several pathogenic bacteria, including methicillin-resistant S. aureus and vancomycin-resistant Enterococci. Epilancin 15X contains an unusual N-terminal lactate group (Lac) that could be important for its bioactivity. To understand its biosynthesis, the epilancin 15X gene cluster was identified. The Lac group is introduced by dehydration of a Ser residue in the first position of the core peptide by a lantibiotic dehydratase (ElxB), followed by proteolytic removal of the leader peptide by a serine-type protease (ElxP), and hydrolysis of the resulting N-terminal dehydroalanine residue. A pyruvyl group thus formed is finally reduced to Lac by a dehydrogenase (ElxO). Using substrate analogs synthesized by solid-phase peptide synthesis or heterologously expressed in Escherichia coli, the enzymatic activities of ElxO and ElxP and their substrate specificities were investigated in vitro. Furthermore, the dehydratase activity of ElxB was demonstrated in vivo. ElxO tolerates several structural modifications along the substrate sequence and can catalyze the NADPH-dependent reduction of diverse pyruvyl- and oxobutyryl-containing peptides, such as the lantibiotic lactocin S, to the corresponding alcohols. The reaction mechanism of ElxO was proposed based on its x ray crystal structure and a mutagenesis study. The characterization of ElxP demonstrated that the protease can cleave the unmodified precursor peptide and relies on the C-terminal residues of the leader region for substrate recognition. Finally, the stereochemical configuration and the importance of the N-terminal Lac for peptide stability against bacterial aminopeptidases were determined. Understanding the biosynthetic route to epilancin 15X opens the door for engineering of novel lantibiotics with more potent biological activities or different spectra of action.","Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2011-10-18T15:02:04Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Velasquez_Juan.pdf: 12538887 bytes, checksum: 392727f6473ab9f9fa5f3640fb5da4fd (MD5)","Made available in DSpace on 2012-02-01T00:54:48Z (GMT). No. of bitstreams: 2 Velasquez_Juan.pdf: 12538861 bytes, checksum: 762e1793a73cfe61cf610250644ef9ce (MD5) license.txt: 4064 bytes, checksum: f56c2674659392401d70833a4b3c331a (MD5)","Item marked as restricted to the 'Administrator' Group (id=1) by William Ingram (wingram2@illinois.edu) on 2012-02-01T00:57:07Z Item is restricted until 2014-02-01T00:56:58Z","Item reinstated by Sarah Shreeves (sshreeve@illinois.edu) on 2014-02-01T11:00:23Z Item was in collections: Dissertations and Theses - Chemistry (ID: 676) Graduate Theses and Dissertations at Illinois (ID: 204) No. of bitstreams: 2 Velasquez_Juan.pdf: 12538861 bytes, checksum: 762e1793a73cfe61cf610250644ef9ce (MD5) license.txt: 4064 bytes, checksum: f56c2674659392401d70833a4b3c331a (MD5)","Item released from any restrictions by Sarah Shreeves (sshreeve@illinois.edu) on 2014-02-01T11:00:23Z"]},{"key":"dc:title","label":"Title","values":["Biosynthesis of the antimicrobial peptide epilancin 15X"]}]}],"canonical_facts":{"dc:contributor":["van der Donk, Wilfred A.","Metcalf, William W.","Oldfield, Eric","Silverman, Scott K."],"dc:creator":["Velasquez, Juan"],"dc:date":["2012-02-01T00:54:48Z","2014-02-01T11:00:23Z","2011-12"],"dc:description":["Lantibiotics are polycyclic antimicrobial peptides that are ribosomally synthesized and posttranslationally modified to their biologically active forms. The recently discovered lantibiotic epilancin 15X produced by Staphylococcus epidermidis 15X154 is active against several pathogenic bacteria, including methicillin-resistant S. aureus and vancomycin-resistant Enterococci. Epilancin 15X contains an unusual N-terminal lactate group (Lac) that could be important for its bioactivity. To understand its biosynthesis, the epilancin 15X gene cluster was identified. The Lac group is introduced by dehydration of a Ser residue in the first position of the core peptide by a lantibiotic dehydratase (ElxB), followed by proteolytic removal of the leader peptide by a serine-type protease (ElxP), and hydrolysis of the resulting N-terminal dehydroalanine residue. A pyruvyl group thus formed is finally reduced to Lac by a dehydrogenase (ElxO). Using substrate analogs synthesized by solid-phase peptide synthesis or heterologously expressed in Escherichia coli, the enzymatic activities of ElxO and ElxP and their substrate specificities were investigated in vitro. Furthermore, the dehydratase activity of ElxB was demonstrated in vivo. ElxO tolerates several structural modifications along the substrate sequence and can catalyze the NADPH-dependent reduction of diverse pyruvyl- and oxobutyryl-containing peptides, such as the lantibiotic lactocin S, to the corresponding alcohols. The reaction mechanism of ElxO was proposed based on its x ray crystal structure and a mutagenesis study. The characterization of ElxP demonstrated that the protease can cleave the unmodified precursor peptide and relies on the C-terminal residues of the leader region for substrate recognition. Finally, the stereochemical configuration and the importance of the N-terminal Lac for peptide stability against bacterial aminopeptidases were determined. Understanding the biosynthetic route to epilancin 15X opens the door for engineering of novel lantibiotics with more potent biological activities or different spectra of action.","Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2011-10-18T15:02:04Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Velasquez_Juan.pdf: 12538887 bytes, checksum: 392727f6473ab9f9fa5f3640fb5da4fd (MD5)","Made available in DSpace on 2012-02-01T00:54:48Z (GMT). No. of bitstreams: 2 Velasquez_Juan.pdf: 12538861 bytes, checksum: 762e1793a73cfe61cf610250644ef9ce (MD5) license.txt: 4064 bytes, checksum: f56c2674659392401d70833a4b3c331a (MD5)","Item marked as restricted to the 'Administrator' Group (id=1) by William Ingram (wingram2@illinois.edu) on 2012-02-01T00:57:07Z Item is restricted until 2014-02-01T00:56:58Z","Item reinstated by Sarah Shreeves (sshreeve@illinois.edu) on 2014-02-01T11:00:23Z Item was in collections: Dissertations and Theses - Chemistry (ID: 676) Graduate Theses and Dissertations at Illinois (ID: 204) No. of bitstreams: 2 Velasquez_Juan.pdf: 12538861 bytes, checksum: 762e1793a73cfe61cf610250644ef9ce (MD5) license.txt: 4064 bytes, checksum: f56c2674659392401d70833a4b3c331a (MD5)","Item released from any restrictions by Sarah Shreeves (sshreeve@illinois.edu) on 2014-02-01T11:00:23Z"],"dc:identifier":["http://hdl.handle.net/2142/29545"],"dc:language":["en"],"dc:rights":["Copyright 2011 Juan Esteban Velásquez"],"dc:subject":["Antibiotics","Lantibiotics","Epilancin 15X","Nisin","Antimicrobial peptides","Biosynthesis","Natural Products","Short-Chain Dehydrogenase/Reductase","Serine proteases","Phosphonates"],"dc:title":["Biosynthesis of the antimicrobial peptide epilancin 15X"],"dc:type":["Dissertation / Thesis","text"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:27Z"}