{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/24451"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/24451","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"The identification and characterization of novel antibacterial compounds via target-based and whole cell screening approaches","abstract":"Item withdrawn by Alexis Thompson (athmpsn1@illinois.edu) on 2011-01-19T21:59:21Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Wang_Nora.pdf: 12154315 bytes, checksum: a4abc355201eaebcd098c4d086069f75 (MD5)","abstract_html":"Item withdrawn by Alexis Thompson (athmpsn1@illinois.edu) on 2011-01-19T21:59:21Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Wang_Nora.pdf: 12154315 bytes, checksum: a4abc355201eaebcd098c4d086069f75 (MD5)","abstract_has_math":false,"creators":["Wang, Nora R."],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Hergenrother, Paul J.","van der Donk, Wilfred A.","Silverman, Scott K.","Burke, Martin D."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-25T14:25:16Z","date_published":"2011-05-25T14:25:16Z","updated_at":"2026-07-22T22:25:23Z","subjects":["antibiotic","antibacterial","Infection","resistance","Staphylococcus aureus"],"languages":["en"],"rights":["Copyright 2011 Nora R. Wang"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/24451","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Hergenrother, Paul J.","van der Donk, Wilfred A.","Silverman, Scott K.","Burke, Martin D."]},{"key":"dc:creator","label":"Author","values":["Wang, Nora R."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-25T14:25:16Z","2013-05-26T10:00:17Z","2011-05"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["antibiotic","antibacterial","Infection","resistance","Staphylococcus aureus"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2011 Nora R. Wang"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/24451"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Item withdrawn by Alexis Thompson (athmpsn1@illinois.edu) on 2011-01-19T21:59:21Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Wang_Nora.pdf: 12154315 bytes, checksum: a4abc355201eaebcd098c4d086069f75 (MD5)","Made available in DSpace on 2011-05-25T14:25:16Z (GMT). No. of bitstreams: 2 Wang_Nora.pdf: 12156650 bytes, checksum: 3fd0ee6f89d2f39fe7c70d4f8b62eeab (MD5) license.txt: 4058 bytes, checksum: 56e6c35dfedf56070d367540e64bf1db (MD5)","Item marked as restricted to the 'Administrator' Group (id=1) by William Ingram (wingram2@illinois.edu) on 2011-05-25T14:29:41Z Item is restricted until 2013-05-25T14:29:35Z","Item reinstated by Sarah Shreeves (sshreeve@illinois.edu) on 2013-05-26T10:00:16Z Item was in collections: University of Illinois Dissertations and Theses (ID: 204) Dissertations and Theses - Chemistry (ID: 676) No. of bitstreams: 3 Wang_Nora.pdf.txt: 408326 bytes, checksum: 9b01a2bf6b2da9ca5a77612f3c1c8b32 (MD5) Wang_Nora.pdf: 12156650 bytes, checksum: 3fd0ee6f89d2f39fe7c70d4f8b62eeab (MD5) license.txt: 4058 bytes, checksum: 56e6c35dfedf56070d367540e64bf1db (MD5)","Item released from any restrictions by Sarah Shreeves (sshreeve@illinois.edu) on 2013-05-26T10:00:17Z","The emergence of multidrug-resistant bacterial infections in both the clinical setting and the community has created an environment in which the development of novel antibacterial compounds is necessary to keep dangerous infections at bay. While the derivatization of existing antibiotics by pharmaceutical companies has so far been successful at achieving this end, this strategy is short-term, and the discovery of antibacterials with novel scaffolds would be a greater contribution to the fight of multidrug-resistant infections. Described herein is the application of both target-based and whole cell screening strategies to identify novel antibacterial compounds. In a target-based approach, we sought small-molecule disruptors of the MazEF toxin-antitoxin protein complex. A lack of facile, continuous assays for this target required the development of a fluorometric assay for MazF ribonuclease activity. This assay was employed to further characterize the activity of the MazF enzyme and was used in a screening effort to identify disruptors of the MazEF complex. In addition, by employing a whole cell screening approach, we identified two compounds with potent antibacterial activity. Efforts to characterize the in vitro antibacterial activities displayed by these compounds and to identify their modes of action are described."]},{"key":"dc:title","label":"Title","values":["The identification and characterization of novel antibacterial compounds via target-based and whole cell screening approaches"]}]}],"canonical_facts":{"dc:contributor":["Hergenrother, Paul J.","van der Donk, Wilfred A.","Silverman, Scott K.","Burke, Martin D."],"dc:creator":["Wang, Nora R."],"dc:date":["2011-05-25T14:25:16Z","2013-05-26T10:00:17Z","2011-05"],"dc:description":["Item withdrawn by Alexis Thompson (athmpsn1@illinois.edu) on 2011-01-19T21:59:21Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Wang_Nora.pdf: 12154315 bytes, checksum: a4abc355201eaebcd098c4d086069f75 (MD5)","Made available in DSpace on 2011-05-25T14:25:16Z (GMT). No. of bitstreams: 2 Wang_Nora.pdf: 12156650 bytes, checksum: 3fd0ee6f89d2f39fe7c70d4f8b62eeab (MD5) license.txt: 4058 bytes, checksum: 56e6c35dfedf56070d367540e64bf1db (MD5)","Item marked as restricted to the 'Administrator' Group (id=1) by William Ingram (wingram2@illinois.edu) on 2011-05-25T14:29:41Z Item is restricted until 2013-05-25T14:29:35Z","Item reinstated by Sarah Shreeves (sshreeve@illinois.edu) on 2013-05-26T10:00:16Z Item was in collections: University of Illinois Dissertations and Theses (ID: 204) Dissertations and Theses - Chemistry (ID: 676) No. of bitstreams: 3 Wang_Nora.pdf.txt: 408326 bytes, checksum: 9b01a2bf6b2da9ca5a77612f3c1c8b32 (MD5) Wang_Nora.pdf: 12156650 bytes, checksum: 3fd0ee6f89d2f39fe7c70d4f8b62eeab (MD5) license.txt: 4058 bytes, checksum: 56e6c35dfedf56070d367540e64bf1db (MD5)","Item released from any restrictions by Sarah Shreeves (sshreeve@illinois.edu) on 2013-05-26T10:00:17Z","The emergence of multidrug-resistant bacterial infections in both the clinical setting and the community has created an environment in which the development of novel antibacterial compounds is necessary to keep dangerous infections at bay. While the derivatization of existing antibiotics by pharmaceutical companies has so far been successful at achieving this end, this strategy is short-term, and the discovery of antibacterials with novel scaffolds would be a greater contribution to the fight of multidrug-resistant infections. Described herein is the application of both target-based and whole cell screening strategies to identify novel antibacterial compounds. In a target-based approach, we sought small-molecule disruptors of the MazEF toxin-antitoxin protein complex. A lack of facile, continuous assays for this target required the development of a fluorometric assay for MazF ribonuclease activity. This assay was employed to further characterize the activity of the MazF enzyme and was used in a screening effort to identify disruptors of the MazEF complex. In addition, by employing a whole cell screening approach, we identified two compounds with potent antibacterial activity. Efforts to characterize the in vitro antibacterial activities displayed by these compounds and to identify their modes of action are described."],"dc:identifier":["http://hdl.handle.net/2142/24451"],"dc:language":["en"],"dc:rights":["Copyright 2011 Nora R. Wang"],"dc:subject":["antibiotic","antibacterial","Infection","resistance","Staphylococcus aureus"],"dc:title":["The identification and characterization of novel antibacterial compounds via target-based and whole cell screening approaches"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:23Z"}