{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/23806"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/23806","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Electron-spin resonance oximetry in cellular systems and in vivo","abstract":"In this thesis, techniques of electron spin resonance (ESR) were developed and applied to measurement of oxygen concentrations in cellular suspensions as well as in vivo systems.","abstract_html":"In this thesis, techniques of electron spin resonance (ESR) were developed and applied to measurement of oxygen concentrations in cellular suspensions as well as in vivo systems.","abstract_has_math":false,"creators":["Glockner, James Frederick"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biophysics, General","degree_department":null,"school":null,"contributors":["Swartz, Harold M."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T14:27:47Z","date_published":"2011-05-07T14:27:47Z","updated_at":"2026-07-22T22:25:22Z","subjects":["Biophysics, General"],"languages":["eng"],"rights":["Copyright 1991 Glockner, James Frederick"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9210815","(UMI)AAI9210815"],"render_values":[{"text":"AAI9210815","href":null,"code":true},{"text":"(UMI)AAI9210815","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/23806","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Swartz, Harold M."]},{"key":"dc:creator","label":"Author","values":["Glockner, James Frederick"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T14:27:47Z","10000-01-01","1991"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biophysics, General"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biophysics, General"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1991 Glockner, James Frederick"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/23806","AAI9210815","(UMI)AAI9210815"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["In this thesis, techniques of electron spin resonance (ESR) were developed and applied to measurement of oxygen concentrations in cellular suspensions as well as in vivo systems.","ESR oximetry was used to measure extracellular and intracellular oxygen concentrations in suspensions of CHO cells. No difference was noted between the extracellular and intracellular values of cells in their baseline state. However, when cellular respiration was stimulated with an uncoupler of oxidative phosphorylation, significant oxygen gradients developed, and these gradients increased as the cellular oxygen consumption rate increased. Attempts to fit these data to standard models of oxygen diffusion and consumption were largely unsuccessful, even when oxygen diffusion barriers at the plasma membrane were invoked.","A technique for measuring the concentration of extracellular oxygen using liposome-encapsulated nitroxides was developed, and it is shown that this method yields results which are similar to those obtained using other well established ESR oximetric techniques. The liposome system was used to measure extracellular oxygen in environments where free nitroxides are rapidly reduced, and it was then applied to measure intracellular and extracellular oxygen concentrations simultaneously in the same sample. The simultaneous technique was applied to suspensions of rapidly respiring myoblast cells, and significant oxygen gradients are demonstrated in unstimulated cells.","In vivo oxygen concentration measurements can be performed with a low frequency (1.1 GHz) ESR spectrometer and surface probe. Liposomes containing the oxygen-sensitive nitroxide d-Cat$\\sb1$ were injected into mouse skeletal muscle, and changes in the local oxygen tension were measured as the fraction of inspired oxygen was varied.","Finally, two novel ESR oxygen probes, fusinite and lithium phthaloryanine, were characterized and then applied to the measurement of in vivo skeletal muscle oxygen tensions. Again, the fraction of inspired oxygen was varied, and changes in tissue pO$\\sb2$ noted. The results of the three in vivo methods were compared, and issues of accuracy, calibration, and oxygen sensitivity discussed.","Made available in DSpace on 2011-05-07T14:27:47Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9210815.pdf: 7025715 bytes, checksum: 85505021503b7b0eb8a12a5885dbd046 (MD5) Previous issue date: 1991","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T15:06:58Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:32:11-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Electron-spin resonance oximetry in cellular systems and in vivo"]}]}],"canonical_facts":{"dc:contributor":["Swartz, Harold M."],"dc:creator":["Glockner, James Frederick"],"dc:date":["2011-05-07T14:27:47Z","10000-01-01","1991"],"dc:description":["In this thesis, techniques of electron spin resonance (ESR) were developed and applied to measurement of oxygen concentrations in cellular suspensions as well as in vivo systems.","ESR oximetry was used to measure extracellular and intracellular oxygen concentrations in suspensions of CHO cells. No difference was noted between the extracellular and intracellular values of cells in their baseline state. However, when cellular respiration was stimulated with an uncoupler of oxidative phosphorylation, significant oxygen gradients developed, and these gradients increased as the cellular oxygen consumption rate increased. Attempts to fit these data to standard models of oxygen diffusion and consumption were largely unsuccessful, even when oxygen diffusion barriers at the plasma membrane were invoked.","A technique for measuring the concentration of extracellular oxygen using liposome-encapsulated nitroxides was developed, and it is shown that this method yields results which are similar to those obtained using other well established ESR oximetric techniques. The liposome system was used to measure extracellular oxygen in environments where free nitroxides are rapidly reduced, and it was then applied to measure intracellular and extracellular oxygen concentrations simultaneously in the same sample. The simultaneous technique was applied to suspensions of rapidly respiring myoblast cells, and significant oxygen gradients are demonstrated in unstimulated cells.","In vivo oxygen concentration measurements can be performed with a low frequency (1.1 GHz) ESR spectrometer and surface probe. Liposomes containing the oxygen-sensitive nitroxide d-Cat$\\sb1$ were injected into mouse skeletal muscle, and changes in the local oxygen tension were measured as the fraction of inspired oxygen was varied.","Finally, two novel ESR oxygen probes, fusinite and lithium phthaloryanine, were characterized and then applied to the measurement of in vivo skeletal muscle oxygen tensions. Again, the fraction of inspired oxygen was varied, and changes in tissue pO$\\sb2$ noted. The results of the three in vivo methods were compared, and issues of accuracy, calibration, and oxygen sensitivity discussed.","Made available in DSpace on 2011-05-07T14:27:47Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9210815.pdf: 7025715 bytes, checksum: 85505021503b7b0eb8a12a5885dbd046 (MD5) Previous issue date: 1991","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T15:06:58Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:32:11-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["http://hdl.handle.net/2142/23806","AAI9210815","(UMI)AAI9210815"],"dc:language":["eng"],"dc:rights":["Copyright 1991 Glockner, James Frederick"],"dc:subject":["Biophysics, General"],"dc:title":["Electron-spin resonance oximetry in cellular systems and in vivo"],"dc:type":["text"],"thesis:degree_discipline":["Biophysics, General"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:22Z"}