{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/23768"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/23768","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Plant regeneration in vitro, characterization and control of vitrification, and somaclonal variation in onion (Allium cepa L.)","abstract":"Restriction data tranferred 2014-07-01T11:32:03-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","abstract_html":"Restriction data tranferred 2014-07-01T11:32:03-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","abstract_has_math":false,"creators":["Schloupt, Renee Marie"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Agriculture, Plant Culture","degree_department":null,"school":null,"contributors":["Splittstoesser, Walter E."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T14:26:21Z","date_published":"2011-05-07T14:26:21Z","updated_at":"2026-07-22T22:25:22Z","subjects":["Agriculture, Plant Culture"],"languages":["eng"],"rights":["Copyright 1994 Schloupt, Renee Marie"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9512541","(UMI)AAI9512541"],"render_values":[{"text":"AAI9512541","href":null,"code":true},{"text":"(UMI)AAI9512541","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/23768","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Splittstoesser, Walter E."]},{"key":"dc:creator","label":"Author","values":["Schloupt, Renee Marie"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T14:26:21Z","10000-01-01","1994"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Agriculture, Plant Culture"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Agriculture, Plant Culture"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1994 Schloupt, Renee Marie"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9512541","(UMI)AAI9512541","http://hdl.handle.net/2142/23768"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Restriction data tranferred 2014-07-01T11:32:03-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only","Several systems were tested for the induction of vitrification so that causes could be studied and possible prevention measures developed. High levels of vitrification were obtained when explants were placed on cosmetic puffs suspended in liquid medium or on medium solidified with low agar levels. Vitrification also significantly increased when decreasing concentrations of gelrite were used to solidify the medium. BA concentrations ranging from 0 to 71 uM had no effect on vitrification level. When varying BA, gelrite and agar concentrations were combined in a factorial design, there was no significant difference in vitrification between individual BA and gelling agent combinations.","Scanning electron microscope studies revealed a difference in leaf morphology between vitrified onion leaves, normal micropropagated leaves and leaves from glasshouse-grown plants. Vitrified leaves appeared to have little epicuticular wax. Stomata were raised above the epidermis, round in shape, and many had malformed openings.","Vitrification could not be eliminated from onion tissue cultures. However, adding activated charcoal to the tissue culture medium or placing explants in a small desiccator containing silica gel significantly reduced percentage vitrification over control plants.","Varying concentrations of the fungicides Captan and Benomyl had no significant effect on the level of fungal contamination or plant growth in onion tissue cultures.","There is little problem removing onion plants from tissue culture and acclimating them to glasshouse conditions. Significant differences were found between onion clones for foliage leaf and bulb ring number. However, these measures may be unreliable due to a fungal infection, which inhibited growth. Flow cytometry is a fast, efficient alternative to chromosome counting to determine ploidy levels. Cytometric analysis revealed one chimeric multiploid in clone 117. Statistical analysis of the G1 DNA content of the clones revealed the possibility of chromosomal abnormalities such as breakages and deletions in several of the clones.","Made available in DSpace on 2011-05-07T14:26:21Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9512541.pdf: 4434249 bytes, checksum: 65af0353bc79f890b500df97b195bc51 (MD5) Previous issue date: 1994","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T15:06:42Z Item is restricted indefinitely."]},{"key":"dc:title","label":"Title","values":["Plant regeneration in vitro, characterization and control of vitrification, and somaclonal variation in onion (Allium cepa L.)"]}]}],"canonical_facts":{"dc:contributor":["Splittstoesser, Walter E."],"dc:creator":["Schloupt, Renee Marie"],"dc:date":["2011-05-07T14:26:21Z","10000-01-01","1994"],"dc:description":["Restriction data tranferred 2014-07-01T11:32:03-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only","Several systems were tested for the induction of vitrification so that causes could be studied and possible prevention measures developed. High levels of vitrification were obtained when explants were placed on cosmetic puffs suspended in liquid medium or on medium solidified with low agar levels. Vitrification also significantly increased when decreasing concentrations of gelrite were used to solidify the medium. BA concentrations ranging from 0 to 71 uM had no effect on vitrification level. When varying BA, gelrite and agar concentrations were combined in a factorial design, there was no significant difference in vitrification between individual BA and gelling agent combinations.","Scanning electron microscope studies revealed a difference in leaf morphology between vitrified onion leaves, normal micropropagated leaves and leaves from glasshouse-grown plants. Vitrified leaves appeared to have little epicuticular wax. Stomata were raised above the epidermis, round in shape, and many had malformed openings.","Vitrification could not be eliminated from onion tissue cultures. However, adding activated charcoal to the tissue culture medium or placing explants in a small desiccator containing silica gel significantly reduced percentage vitrification over control plants.","Varying concentrations of the fungicides Captan and Benomyl had no significant effect on the level of fungal contamination or plant growth in onion tissue cultures.","There is little problem removing onion plants from tissue culture and acclimating them to glasshouse conditions. Significant differences were found between onion clones for foliage leaf and bulb ring number. However, these measures may be unreliable due to a fungal infection, which inhibited growth. Flow cytometry is a fast, efficient alternative to chromosome counting to determine ploidy levels. Cytometric analysis revealed one chimeric multiploid in clone 117. Statistical analysis of the G1 DNA content of the clones revealed the possibility of chromosomal abnormalities such as breakages and deletions in several of the clones.","Made available in DSpace on 2011-05-07T14:26:21Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9512541.pdf: 4434249 bytes, checksum: 65af0353bc79f890b500df97b195bc51 (MD5) Previous issue date: 1994","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T15:06:42Z Item is restricted indefinitely."],"dc:identifier":["AAI9512541","(UMI)AAI9512541","http://hdl.handle.net/2142/23768"],"dc:language":["eng"],"dc:rights":["Copyright 1994 Schloupt, Renee Marie"],"dc:subject":["Agriculture, Plant Culture"],"dc:title":["Plant regeneration in vitro, characterization and control of vitrification, and somaclonal variation in onion (Allium cepa L.)"],"dc:type":["text"],"thesis:degree_discipline":["Agriculture, Plant Culture"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:22Z"}