{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/23552"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/23552","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Amiloride-sensitive components of sodium transport in guinea pig red blood cells: A cause of imbalance of sodium ion at low temperature","abstract":"It has previously been found that gaining of Na in red cells of guinea pig is faster than in those of ground squirrel during cold storage at 5$\\sp\\circ$C in vitro and amiloride can partially inhibit the accumulation. Also, amiloride-sensitive Na influx in guinea pig red cells is known to be larger at 20$\\sp\\circ$C than at 37$\\sp\\circ$C.","abstract_html":"It has previously been found that gaining of Na in red cells of guinea pig is faster than in those of ground squirrel during cold storage at 5$\\sp\\circ$C in vitro and amiloride can partially inhibit the accumulation. Also, amiloride-sensitive Na influx in guinea pig red cells is known to be larger at 20$\\sp\\circ$C than at 37$\\sp\\circ$C.","abstract_has_math":true,"creators":["Zhao, Zhihong"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biology, Animal Physiology","degree_department":null,"school":null,"contributors":["Willis, John S."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T14:18:24Z","date_published":"2011-05-07T14:18:24Z","updated_at":"2026-07-22T22:25:22Z","subjects":["Biology, Animal Physiology","Biology, Zoology"],"languages":["eng"],"rights":["Copyright 1991 Zhao, Zhihong"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9211058","(UMI)AAI9211058"],"render_values":[{"text":"AAI9211058","href":null,"code":true},{"text":"(UMI)AAI9211058","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/23552","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Willis, John S."]},{"key":"dc:creator","label":"Author","values":["Zhao, Zhihong"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T14:18:24Z","10000-01-01","1991"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology, Animal Physiology","Biology, Zoology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Animal Physiology","Biology, Zoology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1991 Zhao, Zhihong"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9211058","(UMI)AAI9211058","http://hdl.handle.net/2142/23552"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["It has previously been found that gaining of Na in red cells of guinea pig is faster than in those of ground squirrel during cold storage at 5$\\sp\\circ$C in vitro and amiloride can partially inhibit the accumulation. Also, amiloride-sensitive Na influx in guinea pig red cells is known to be larger at 20$\\sp\\circ$C than at 37$\\sp\\circ$C.","In this study, it is shown that amiloride-sensitive Na influx in guinea pig red blood cells can be activated by cytoplasmic H$\\sp+$ and it is correlated with cation-dependent H$\\sp+$ loss from acidified cells. It is also stimulated by shrinkage and by phorbol ester in the presence of Ca, as is Na-H exchange in other cells. Aside from this apparent Na-H exchange, there are at least one and possibly two other distinct components of amiloride-sensitive Na influx: one is hypertonically induced, with a high sensitivity to amiloride and with low sensitivity to Na; the other appears to represent Na-Mg exchange.","Comparison between results at 37$\\sp\\circ$C and 20$\\sp\\circ$C shows that amiloride-sensitive Na influx can be activated dramatically by the activators of Na-H exchange at 37$\\sp\\circ$C, but not at 20$\\sp\\circ$C. Cell alkalinization can diminish the elevation of amiloride-sensitive Na influx at 20$\\sp\\circ$C, while depletion of cell magnesium can not. These observations lead to the conclusion that cooling-induced amiloride-sensitive Na influx in guinea pig cells is mainly due to the increased activity of the Na-H exchange mechanism.","\"By measurement of the stimulation of amiloride-sensitive Na influx by cytoplasmic H$\\sp+,$ the affinity of the amiloride-sensitive Na-H exchanger to cytoplasmic H$\\sp+$ is found to be higher at 20$\\sp\\circ$C than at 37$\\sp\\circ$C. In cells incubated in Na-free medium at 37$\\sp\\circ$C (\"\"reversed Na gradient\"\"), acidification increased amiloride-sensitive Na efflux, indicating the presence of a cytoplasmic regulatory site for H$\\sp+.$ At 20$\\sp\\circ$C, acidification caused no significant increase, but basification inhibited Na efflux into Na-free medium, indicating higher sensitivity of the regulatory site to cytoplasmic H$\\sp+.$ Thus increased activity of the Na-H exchange at reduced temperature is characterized by reduced H$\\sp+$ transport but increased affinity of a regulatory site for H$\\sp+.$\"","Made available in DSpace on 2011-05-07T14:18:24Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9211058.pdf: 3877003 bytes, checksum: dc19f6f52727abb523ca9963e5d0039d (MD5) Previous issue date: 1991","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T15:05:14Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:31:14-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Amiloride-sensitive components of sodium transport in guinea pig red blood cells: A cause of imbalance of sodium ion at low temperature"]}]}],"canonical_facts":{"dc:contributor":["Willis, John S."],"dc:creator":["Zhao, Zhihong"],"dc:date":["2011-05-07T14:18:24Z","10000-01-01","1991"],"dc:description":["It has previously been found that gaining of Na in red cells of guinea pig is faster than in those of ground squirrel during cold storage at 5$\\sp\\circ$C in vitro and amiloride can partially inhibit the accumulation. Also, amiloride-sensitive Na influx in guinea pig red cells is known to be larger at 20$\\sp\\circ$C than at 37$\\sp\\circ$C.","In this study, it is shown that amiloride-sensitive Na influx in guinea pig red blood cells can be activated by cytoplasmic H$\\sp+$ and it is correlated with cation-dependent H$\\sp+$ loss from acidified cells. It is also stimulated by shrinkage and by phorbol ester in the presence of Ca, as is Na-H exchange in other cells. Aside from this apparent Na-H exchange, there are at least one and possibly two other distinct components of amiloride-sensitive Na influx: one is hypertonically induced, with a high sensitivity to amiloride and with low sensitivity to Na; the other appears to represent Na-Mg exchange.","Comparison between results at 37$\\sp\\circ$C and 20$\\sp\\circ$C shows that amiloride-sensitive Na influx can be activated dramatically by the activators of Na-H exchange at 37$\\sp\\circ$C, but not at 20$\\sp\\circ$C. Cell alkalinization can diminish the elevation of amiloride-sensitive Na influx at 20$\\sp\\circ$C, while depletion of cell magnesium can not. These observations lead to the conclusion that cooling-induced amiloride-sensitive Na influx in guinea pig cells is mainly due to the increased activity of the Na-H exchange mechanism.","\"By measurement of the stimulation of amiloride-sensitive Na influx by cytoplasmic H$\\sp+,$ the affinity of the amiloride-sensitive Na-H exchanger to cytoplasmic H$\\sp+$ is found to be higher at 20$\\sp\\circ$C than at 37$\\sp\\circ$C. In cells incubated in Na-free medium at 37$\\sp\\circ$C (\"\"reversed Na gradient\"\"), acidification increased amiloride-sensitive Na efflux, indicating the presence of a cytoplasmic regulatory site for H$\\sp+.$ At 20$\\sp\\circ$C, acidification caused no significant increase, but basification inhibited Na efflux into Na-free medium, indicating higher sensitivity of the regulatory site to cytoplasmic H$\\sp+.$ Thus increased activity of the Na-H exchange at reduced temperature is characterized by reduced H$\\sp+$ transport but increased affinity of a regulatory site for H$\\sp+.$\"","Made available in DSpace on 2011-05-07T14:18:24Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9211058.pdf: 3877003 bytes, checksum: dc19f6f52727abb523ca9963e5d0039d (MD5) Previous issue date: 1991","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T15:05:14Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:31:14-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9211058","(UMI)AAI9211058","http://hdl.handle.net/2142/23552"],"dc:language":["eng"],"dc:rights":["Copyright 1991 Zhao, Zhihong"],"dc:subject":["Biology, Animal Physiology","Biology, Zoology"],"dc:title":["Amiloride-sensitive components of sodium transport in guinea pig red blood cells: A cause of imbalance of sodium ion at low temperature"],"dc:type":["text"],"thesis:degree_discipline":["Biology, Animal Physiology","Biology, Zoology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:22Z"}