{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/23540"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/23540","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Characterization of six photoautotrophic plant cell lines and assessment of changes after long-term continuous culture","abstract":"Made available in DSpace on 2011-05-07T14:18:00Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9503198.pdf: 4832446 bytes, checksum: 561998f59f31bfc6ae6319424788015c (MD5) Previous issue date: 1994","abstract_html":"Made available in DSpace on 2011-05-07T14:18:00Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9503198.pdf: 4832446 bytes, checksum: 561998f59f31bfc6ae6319424788015c (MD5) Previous issue date: 1994","abstract_has_math":false,"creators":["Goldstein, Cindy Susan"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biology, Botany","degree_department":null,"school":null,"contributors":["Widholm, Jack M."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T14:18:00Z","date_published":"2011-05-07T14:18:00Z","updated_at":"2026-07-22T22:25:22Z","subjects":["Biology, Botany","Agriculture, Plant Culture","Biology, Plant Physiology"],"languages":["eng"],"rights":["Copyright 1994 Goldstein, Cindy Susan"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9503198","(UMI)AAI9503198"],"render_values":[{"text":"AAI9503198","href":null,"code":true},{"text":"(UMI)AAI9503198","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/23540","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Widholm, Jack M."]},{"key":"dc:creator","label":"Author","values":["Goldstein, Cindy Susan"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T14:18:00Z","10000-01-01","1994"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology, Botany","Agriculture, Plant Culture","Biology, Plant Physiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Botany","Agriculture, Plant Culture","Biology, Plant Physiology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1994 Goldstein, Cindy Susan"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9503198","(UMI)AAI9503198","http://hdl.handle.net/2142/23540"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Made available in DSpace on 2011-05-07T14:18:00Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9503198.pdf: 4832446 bytes, checksum: 561998f59f31bfc6ae6319424788015c (MD5) Previous issue date: 1994","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T15:05:10Z Item is restricted indefinitely.","Photoautotrophic cells are chlorophyllous plant cells grown with no sugars and minimal or no organic carbon in the culture medium. These cells are photosynthetic and are capable of light-dependent CO$\\sb2$ fixation. Our laboratory has initiated and continued to grow several different species of photoautotrophs under strict photoautotrophic conditions including Amaranthus powellii, Datura innoxia, Glycine max, and Gossypium hirsutum cell lines, as well as a Nicotiana tabacum-glutinosa fusion hybrid line. These cells have high levels of chiropodies, ranging from approximately 500 $\\mu$g/g fresh weight for the photoautotrophic Nicotiana cells to as high as 4000 $\\mu$g/g fresh weight for G. max and G. hirsutum cells. The chlorophyll contents of these cells have reached levels much higher than that described in the literature for other photoautotrophic culture lines. The photoautotrophic cells primarily carry out C$\\sb3$ photosynthesis, fixing CO$\\sb2$ primarily via RuBP carboxylase through the Calvin Cycle. Culture lines often had high levels of PEP carboxylase activity in the first year following culture initiation, with a decrease in PEP carboxylase activity and an increase in RuBP carboxylase activity over a seven to ten year time period. The ratio of RuBP carboxylase activity to PEP carboxylase activity has likewise increased the longer the cells have been selected for continuous culture in a photoautotrophic environment. The percent activation of RuBP carboxylase has increased from activation levels of below 50% to levels of 70-80% for all of the photoautotrophic culture lines except D. innoxia. The photosynthetic capacity of the cells has increased over a 5 to 7 year time period, as well, with increasing rates of carboxylase activity as chlorophyll content has continued to increase.","Restriction data tranferred 2014-07-01T11:31:12-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Characterization of six photoautotrophic plant cell lines and assessment of changes after long-term continuous culture"]}]}],"canonical_facts":{"dc:contributor":["Widholm, Jack M."],"dc:creator":["Goldstein, Cindy Susan"],"dc:date":["2011-05-07T14:18:00Z","10000-01-01","1994"],"dc:description":["Made available in DSpace on 2011-05-07T14:18:00Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9503198.pdf: 4832446 bytes, checksum: 561998f59f31bfc6ae6319424788015c (MD5) Previous issue date: 1994","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T15:05:10Z Item is restricted indefinitely.","Photoautotrophic cells are chlorophyllous plant cells grown with no sugars and minimal or no organic carbon in the culture medium. These cells are photosynthetic and are capable of light-dependent CO$\\sb2$ fixation. Our laboratory has initiated and continued to grow several different species of photoautotrophs under strict photoautotrophic conditions including Amaranthus powellii, Datura innoxia, Glycine max, and Gossypium hirsutum cell lines, as well as a Nicotiana tabacum-glutinosa fusion hybrid line. These cells have high levels of chiropodies, ranging from approximately 500 $\\mu$g/g fresh weight for the photoautotrophic Nicotiana cells to as high as 4000 $\\mu$g/g fresh weight for G. max and G. hirsutum cells. The chlorophyll contents of these cells have reached levels much higher than that described in the literature for other photoautotrophic culture lines. The photoautotrophic cells primarily carry out C$\\sb3$ photosynthesis, fixing CO$\\sb2$ primarily via RuBP carboxylase through the Calvin Cycle. Culture lines often had high levels of PEP carboxylase activity in the first year following culture initiation, with a decrease in PEP carboxylase activity and an increase in RuBP carboxylase activity over a seven to ten year time period. The ratio of RuBP carboxylase activity to PEP carboxylase activity has likewise increased the longer the cells have been selected for continuous culture in a photoautotrophic environment. The percent activation of RuBP carboxylase has increased from activation levels of below 50% to levels of 70-80% for all of the photoautotrophic culture lines except D. innoxia. The photosynthetic capacity of the cells has increased over a 5 to 7 year time period, as well, with increasing rates of carboxylase activity as chlorophyll content has continued to increase.","Restriction data tranferred 2014-07-01T11:31:12-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9503198","(UMI)AAI9503198","http://hdl.handle.net/2142/23540"],"dc:language":["eng"],"dc:rights":["Copyright 1994 Goldstein, Cindy Susan"],"dc:subject":["Biology, Botany","Agriculture, Plant Culture","Biology, Plant Physiology"],"dc:title":["Characterization of six photoautotrophic plant cell lines and assessment of changes after long-term continuous culture"],"dc:type":["text"],"thesis:degree_discipline":["Biology, Botany","Agriculture, Plant Culture","Biology, Plant Physiology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:22Z"}