{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/23224"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/23224","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Identification and characterization of the ribosomal RNA operons from Rhodobacter sphaeroides","abstract":"The three ribosomal RNA operons present in the facultative photoheterotroph Rhodobacter sphaeroides were identified, cloned and sequenced. DNA sequence analysis has identified the 16S, 23S and 5S rRNAs, two tRNAs (ile and ala) in the 16S-23S spacer region, and an f-met tRNA immediately following the 5S rRNa gene of all three operons. The secondary structure of the rRNA and tRNA molecules present in the rRNA operons was deduced and evolutionary implications discussed. Physical mapping, genetic analysis, and Southern hybridization data indicate that rrnA is contained on a large chromosome and rrnB and rrnC are contained on a second smaller chromosome. Strains were constructed in which one or two rRNA operons were deleted and the effects on cell growth analyzed. Strains with deletions in one or two rrn operons grew more slowly than wild type strains under all growth conditions tested. However, there was no significant difference in the growth rate of strains with two rrn operons deleted when compared to strains deleted in only one operon. In addition, the primary transcript of each rRNA operon was identified via primer extension analysis. The region upstream of the primary rRNA transcripts was analyzed and a $-$10 and $-$35 promoter region was identified as well as regulatory elements which may be involved in regulating the synthesis of the rRNA operons. Fusions of the proposed promoter regions were constructed utilizing the reporter molecule xylE and analyzed under various growth conditions. Results indicate that production of the xylE gene product (catechol 2,3-dioxygenase) was greatest under photosynthetic conditions. Also, the upstream region of rrnB, when fused with xylE produced more catechol 2,3-dioxygenase than analogous regions of rrnA, suggesting that the promoters of the rrn operons differ in strength. It is postulated that under the same conditions, the rrnC promoter could potentially be the weakest of the three rrn promoters.","abstract_html":"The three ribosomal RNA operons present in the facultative photoheterotroph Rhodobacter sphaeroides were identified, cloned and sequenced. DNA sequence analysis has identified the 16S, 23S and 5S rRNAs, two tRNAs (ile and ala) in the 16S-23S spacer region, and an f-met tRNA immediately following the 5S rRNa gene of all three operons. The secondary structure of the rRNA and tRNA molecules present in the rRNA operons was deduced and evolutionary implications discussed. Physical mapping, genetic analysis, and Southern hybridization data indicate that rrnA is contained on a large chromosome and rrnB and rrnC are contained on a second smaller chromosome. Strains were constructed in which one or two rRNA operons were deleted and the effects on cell growth analyzed. Strains with deletions in one or two rrn operons grew more slowly than wild type strains under all growth conditions tested. However, there was no significant difference in the growth rate of strains with two rrn operons deleted when compared to strains deleted in only one operon. In addition, the primary transcript of each rRNA operon was identified via primer extension analysis. The region upstream of the primary rRNA transcripts was analyzed and a $-$10 and $-$35 promoter region was identified as well as regulatory elements which may be involved in regulating the synthesis of the rRNA operons. Fusions of the proposed promoter regions were constructed utilizing the reporter molecule xylE and analyzed under various growth conditions. Results indicate that production of the xylE gene product (catechol 2,3-dioxygenase) was greatest under photosynthetic conditions. Also, the upstream region of rrnB, when fused with xylE produced more catechol 2,3-dioxygenase than analogous regions of rrnA, suggesting that the promoters of the rrn operons differ in strength. It is postulated that under the same conditions, the rrnC promoter could potentially be the weakest of the three rrn promoters.","abstract_has_math":true,"creators":["Dryden, Sylvia Cartwright"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":["Kaplan, Samuel"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T14:06:35Z","date_published":"2011-05-07T14:06:35Z","updated_at":"2026-07-22T22:25:21Z","subjects":["Biology, Molecular","Biology, Microbiology"],"languages":["eng"],"rights":["Copyright 1992 Dryden, Sylvia Cartwright"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9236441","(UMI)AAI9236441"],"render_values":[{"text":"AAI9236441","href":null,"code":true},{"text":"(UMI)AAI9236441","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/23224","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Kaplan, Samuel"]},{"key":"dc:creator","label":"Author","values":["Dryden, Sylvia Cartwright"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T14:06:35Z","10000-01-01","1992"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Molecular","Biology, Microbiology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1992 Dryden, Sylvia Cartwright"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9236441","(UMI)AAI9236441","http://hdl.handle.net/2142/23224"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The three ribosomal RNA operons present in the facultative photoheterotroph Rhodobacter sphaeroides were identified, cloned and sequenced. DNA sequence analysis has identified the 16S, 23S and 5S rRNAs, two tRNAs (ile and ala) in the 16S-23S spacer region, and an f-met tRNA immediately following the 5S rRNa gene of all three operons. The secondary structure of the rRNA and tRNA molecules present in the rRNA operons was deduced and evolutionary implications discussed. Physical mapping, genetic analysis, and Southern hybridization data indicate that rrnA is contained on a large chromosome and rrnB and rrnC are contained on a second smaller chromosome. Strains were constructed in which one or two rRNA operons were deleted and the effects on cell growth analyzed. Strains with deletions in one or two rrn operons grew more slowly than wild type strains under all growth conditions tested. However, there was no significant difference in the growth rate of strains with two rrn operons deleted when compared to strains deleted in only one operon. In addition, the primary transcript of each rRNA operon was identified via primer extension analysis. The region upstream of the primary rRNA transcripts was analyzed and a $-$10 and $-$35 promoter region was identified as well as regulatory elements which may be involved in regulating the synthesis of the rRNA operons. Fusions of the proposed promoter regions were constructed utilizing the reporter molecule xylE and analyzed under various growth conditions. Results indicate that production of the xylE gene product (catechol 2,3-dioxygenase) was greatest under photosynthetic conditions. Also, the upstream region of rrnB, when fused with xylE produced more catechol 2,3-dioxygenase than analogous regions of rrnA, suggesting that the promoters of the rrn operons differ in strength. It is postulated that under the same conditions, the rrnC promoter could potentially be the weakest of the three rrn promoters.","Made available in DSpace on 2011-05-07T14:06:35Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9236441.pdf: 6922932 bytes, checksum: b7628aeb22ea6376a97600d85d4e07c4 (MD5) Previous issue date: 1992","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T15:03:01Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:30:01-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Identification and characterization of the ribosomal RNA operons from Rhodobacter sphaeroides"]}]}],"canonical_facts":{"dc:contributor":["Kaplan, Samuel"],"dc:creator":["Dryden, Sylvia Cartwright"],"dc:date":["2011-05-07T14:06:35Z","10000-01-01","1992"],"dc:description":["The three ribosomal RNA operons present in the facultative photoheterotroph Rhodobacter sphaeroides were identified, cloned and sequenced. DNA sequence analysis has identified the 16S, 23S and 5S rRNAs, two tRNAs (ile and ala) in the 16S-23S spacer region, and an f-met tRNA immediately following the 5S rRNa gene of all three operons. The secondary structure of the rRNA and tRNA molecules present in the rRNA operons was deduced and evolutionary implications discussed. Physical mapping, genetic analysis, and Southern hybridization data indicate that rrnA is contained on a large chromosome and rrnB and rrnC are contained on a second smaller chromosome. Strains were constructed in which one or two rRNA operons were deleted and the effects on cell growth analyzed. Strains with deletions in one or two rrn operons grew more slowly than wild type strains under all growth conditions tested. However, there was no significant difference in the growth rate of strains with two rrn operons deleted when compared to strains deleted in only one operon. In addition, the primary transcript of each rRNA operon was identified via primer extension analysis. The region upstream of the primary rRNA transcripts was analyzed and a $-$10 and $-$35 promoter region was identified as well as regulatory elements which may be involved in regulating the synthesis of the rRNA operons. Fusions of the proposed promoter regions were constructed utilizing the reporter molecule xylE and analyzed under various growth conditions. Results indicate that production of the xylE gene product (catechol 2,3-dioxygenase) was greatest under photosynthetic conditions. Also, the upstream region of rrnB, when fused with xylE produced more catechol 2,3-dioxygenase than analogous regions of rrnA, suggesting that the promoters of the rrn operons differ in strength. It is postulated that under the same conditions, the rrnC promoter could potentially be the weakest of the three rrn promoters.","Made available in DSpace on 2011-05-07T14:06:35Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9236441.pdf: 6922932 bytes, checksum: b7628aeb22ea6376a97600d85d4e07c4 (MD5) Previous issue date: 1992","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T15:03:01Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:30:01-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9236441","(UMI)AAI9236441","http://hdl.handle.net/2142/23224"],"dc:language":["eng"],"dc:rights":["Copyright 1992 Dryden, Sylvia Cartwright"],"dc:subject":["Biology, Molecular","Biology, Microbiology"],"dc:title":["Identification and characterization of the ribosomal RNA operons from Rhodobacter sphaeroides"],"dc:type":["text"],"thesis:degree_discipline":["Microbiology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:21Z"}