{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/22986"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/22986","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Characterization of features of the initiation of translation of satellite tobacco necrosis virus RNA","abstract":"The translation of Satellite Tobacco Necrosis Virus (STNV) RNA has been shown to initiate efficiently in the absence of a cap structure and in the absence of an AUG initiation codon. These unusual characteristics of STNV RNA were shown to reside in the 5$\\sp\\prime$-untranslated region by replacing these upstream sequences with either a random sequence or the 5$\\sp\\prime$-untranslated region of brome mosaic virus RNA 4. Such a replacement caused a loss of translation when the mutant RNA was made without a cap. STNV RNA may have a unique 18S ribosomal RNA hybridization site that allows internal initiation of translation to occur. This site was tested by inserting a translation initiation codon upstream of the internal initiation site. Interestingly, the initiation of translation of these mutant RNAs occurs at the upstream site. To explain these results, a possible mechanism involving the internal initiation site is proposed. STNV RNA binds at the 18S rRNA hybridization site and translation initiates at the normal internal initiation codon. This first ribosome binding frees the upstream site from secondary structure allowing all subsequent ribosomes to initiate translation at the upstream AUG codon.","abstract_html":"The translation of Satellite Tobacco Necrosis Virus (STNV) RNA has been shown to initiate efficiently in the absence of a cap structure and in the absence of an AUG initiation codon. These unusual characteristics of STNV RNA were shown to reside in the 5$\\sp\\prime$-untranslated region by replacing these upstream sequences with either a random sequence or the 5$\\sp\\prime$-untranslated region of brome mosaic virus RNA 4. Such a replacement caused a loss of translation when the mutant RNA was made without a cap. STNV RNA may have a unique 18S ribosomal RNA hybridization site that allows internal initiation of translation to occur. This site was tested by inserting a translation initiation codon upstream of the internal initiation site. Interestingly, the initiation of translation of these mutant RNAs occurs at the upstream site. To explain these results, a possible mechanism involving the internal initiation site is proposed. STNV RNA binds at the 18S rRNA hybridization site and translation initiates at the normal internal initiation codon. This first ribosome binding frees the upstream site from secondary structure allowing all subsequent ribosomes to initiate translation at the upstream AUG codon.","abstract_has_math":true,"creators":["Riegel, Christopher Isaac"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biochemistry","degree_department":null,"school":null,"contributors":["Clark, John M., Jr."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T13:58:08Z","date_published":"2011-05-07T13:58:08Z","updated_at":"2026-07-22T22:25:21Z","subjects":["Biology, Molecular","Agriculture, Plant Pathology","Chemistry, Biochemistry"],"languages":["eng"],"rights":["Copyright 1991 Riegel, Christopher Isaac"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9136714","(UMI)AAI9136714"],"render_values":[{"text":"AAI9136714","href":null,"code":true},{"text":"(UMI)AAI9136714","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/22986","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Clark, John M., Jr."]},{"key":"dc:creator","label":"Author","values":["Riegel, Christopher Isaac"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T13:58:08Z","10000-01-01","1991"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Molecular","Agriculture, Plant Pathology","Chemistry, Biochemistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1991 Riegel, Christopher Isaac"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9136714","(UMI)AAI9136714","http://hdl.handle.net/2142/22986"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The translation of Satellite Tobacco Necrosis Virus (STNV) RNA has been shown to initiate efficiently in the absence of a cap structure and in the absence of an AUG initiation codon. These unusual characteristics of STNV RNA were shown to reside in the 5$\\sp\\prime$-untranslated region by replacing these upstream sequences with either a random sequence or the 5$\\sp\\prime$-untranslated region of brome mosaic virus RNA 4. Such a replacement caused a loss of translation when the mutant RNA was made without a cap. STNV RNA may have a unique 18S ribosomal RNA hybridization site that allows internal initiation of translation to occur. This site was tested by inserting a translation initiation codon upstream of the internal initiation site. Interestingly, the initiation of translation of these mutant RNAs occurs at the upstream site. To explain these results, a possible mechanism involving the internal initiation site is proposed. STNV RNA binds at the 18S rRNA hybridization site and translation initiates at the normal internal initiation codon. This first ribosome binding frees the upstream site from secondary structure allowing all subsequent ribosomes to initiate translation at the upstream AUG codon.","Made available in DSpace on 2011-05-07T13:58:08Z (GMT). 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These unusual characteristics of STNV RNA were shown to reside in the 5$\\sp\\prime$-untranslated region by replacing these upstream sequences with either a random sequence or the 5$\\sp\\prime$-untranslated region of brome mosaic virus RNA 4. Such a replacement caused a loss of translation when the mutant RNA was made without a cap. STNV RNA may have a unique 18S ribosomal RNA hybridization site that allows internal initiation of translation to occur. This site was tested by inserting a translation initiation codon upstream of the internal initiation site. Interestingly, the initiation of translation of these mutant RNAs occurs at the upstream site. To explain these results, a possible mechanism involving the internal initiation site is proposed. STNV RNA binds at the 18S rRNA hybridization site and translation initiates at the normal internal initiation codon. This first ribosome binding frees the upstream site from secondary structure allowing all subsequent ribosomes to initiate translation at the upstream AUG codon.","Made available in DSpace on 2011-05-07T13:58:08Z (GMT). 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