{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/22857"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/22857","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Developmental expression of a 4.5S RNA and variants of U1snRNA in Drosophila melanogaster","abstract":"Analysis of small RNAs in Drosophila melanogaster revealed the existence of a 4.5S RNA and variants for the U1 snRNA. The 4.5S RNA was separated from other snRNAs by electrophoresis through high percentage polyacrylamide gels. The Drosophila 4.5S RNA migrated at the same rate as the mouse 4.5S$\\sb{\\rm I}$ RNA standard. Chemical sequence analysis of the 3$\\sp\\prime$ end of the Drosophila 4.5S RNA revealed a 60% nucleotide homology with the mouse 4.5S$\\sb{\\rm I}$ RNA. The mouse 4.5S$\\sb{\\rm I}$ RNA is related to the B$\\sb2$ repeat of the mouse genome. The existence and homology of the Drosophila 4.5S RNA to the mouse 4.5S$\\sb{\\rm I}$ RNA may provide a focal point for the study of the origins of repeated sequences.","abstract_html":"Analysis of small RNAs in Drosophila melanogaster revealed the existence of a 4.5S RNA and variants for the U1 snRNA. The 4.5S RNA was separated from other snRNAs by electrophoresis through high percentage polyacrylamide gels. The Drosophila 4.5S RNA migrated at the same rate as the mouse 4.5S$\\sb{\\rm I}$ RNA standard. Chemical sequence analysis of the 3$\\sp\\prime$ end of the Drosophila 4.5S RNA revealed a 60% nucleotide homology with the mouse 4.5S$\\sb{\\rm I}$ RNA. The mouse 4.5S$\\sb{\\rm I}$ RNA is related to the B$\\sb2$ repeat of the mouse genome. The existence and homology of the Drosophila 4.5S RNA to the mouse 4.5S$\\sb{\\rm I}$ RNA may provide a focal point for the study of the origins of repeated sequences.","abstract_has_math":true,"creators":["Koehler, Richard Fred"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Steffensen, D.M."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T13:53:49Z","date_published":"2011-05-07T13:53:49Z","updated_at":"2026-07-22T22:25:20Z","subjects":["Biology, Molecular","Biology, Genetics"],"languages":["eng"],"rights":["Copyright 1991 Koehler, Richard Fred"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9136639","(UMI)AAI9136639"],"render_values":[{"text":"AAI9136639","href":null,"code":true},{"text":"(UMI)AAI9136639","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/22857","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Steffensen, D.M."]},{"key":"dc:creator","label":"Author","values":["Koehler, Richard Fred"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T13:53:49Z","10000-01-01","1991"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Molecular","Biology, Genetics"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1991 Koehler, Richard Fred"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9136639","(UMI)AAI9136639","http://hdl.handle.net/2142/22857"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Analysis of small RNAs in Drosophila melanogaster revealed the existence of a 4.5S RNA and variants for the U1 snRNA. The 4.5S RNA was separated from other snRNAs by electrophoresis through high percentage polyacrylamide gels. The Drosophila 4.5S RNA migrated at the same rate as the mouse 4.5S$\\sb{\\rm I}$ RNA standard. Chemical sequence analysis of the 3$\\sp\\prime$ end of the Drosophila 4.5S RNA revealed a 60% nucleotide homology with the mouse 4.5S$\\sb{\\rm I}$ RNA. The mouse 4.5S$\\sb{\\rm I}$ RNA is related to the B$\\sb2$ repeat of the mouse genome. The existence and homology of the Drosophila 4.5S RNA to the mouse 4.5S$\\sb{\\rm I}$ RNA may provide a focal point for the study of the origins of repeated sequences.","The U1 snRNA variants of Drosophila were separated by electrophoresis through high percentage polyacrylamide gels. A comparison of the U1 variants from unfertilized eggs, second and third instar larva, and adult flies revealed developmental differences in their expression. In unfertilized eggs the U1 snRNA is absent or in very low concentration. In second and third instar larva there are at least two variants for the U1 snRNA, while in adult flies there are at least 4 variants. The nature of the variants was not determined; however, each variant hybridized to a U1 snRNA specific probe. Developmental differences were also observed for variants of the U4 and U6 snRNAs. The U2 snRNA of Drosophila showed a large difference in migration rate between third instar larva and adult flies. The adult U2 migrated significantly faster than the third instar U2. Since only one nucleotide sequence is known for the U2 snRNA, the differences in migration may be due to developmentally-regulated base modifications. These snRNA variants may be involved in the alternative splicing of primary transcripts. The snRNA variants may produce functionally-different spliceosomes that splice primary transcripts in a developmental- or tissue-specific manner.","Made available in DSpace on 2011-05-07T13:53:49Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9136639.pdf: 6828224 bytes, checksum: 22cee2501b46590ed01f8956abf70114 (MD5) Previous issue date: 1991","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T15:00:29Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:28:37-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Developmental expression of a 4.5S RNA and variants of U1snRNA in Drosophila melanogaster"]}]}],"canonical_facts":{"dc:contributor":["Steffensen, D.M."],"dc:creator":["Koehler, Richard Fred"],"dc:date":["2011-05-07T13:53:49Z","10000-01-01","1991"],"dc:description":["Analysis of small RNAs in Drosophila melanogaster revealed the existence of a 4.5S RNA and variants for the U1 snRNA. The 4.5S RNA was separated from other snRNAs by electrophoresis through high percentage polyacrylamide gels. The Drosophila 4.5S RNA migrated at the same rate as the mouse 4.5S$\\sb{\\rm I}$ RNA standard. Chemical sequence analysis of the 3$\\sp\\prime$ end of the Drosophila 4.5S RNA revealed a 60% nucleotide homology with the mouse 4.5S$\\sb{\\rm I}$ RNA. The mouse 4.5S$\\sb{\\rm I}$ RNA is related to the B$\\sb2$ repeat of the mouse genome. The existence and homology of the Drosophila 4.5S RNA to the mouse 4.5S$\\sb{\\rm I}$ RNA may provide a focal point for the study of the origins of repeated sequences.","The U1 snRNA variants of Drosophila were separated by electrophoresis through high percentage polyacrylamide gels. A comparison of the U1 variants from unfertilized eggs, second and third instar larva, and adult flies revealed developmental differences in their expression. In unfertilized eggs the U1 snRNA is absent or in very low concentration. In second and third instar larva there are at least two variants for the U1 snRNA, while in adult flies there are at least 4 variants. The nature of the variants was not determined; however, each variant hybridized to a U1 snRNA specific probe. Developmental differences were also observed for variants of the U4 and U6 snRNAs. The U2 snRNA of Drosophila showed a large difference in migration rate between third instar larva and adult flies. The adult U2 migrated significantly faster than the third instar U2. Since only one nucleotide sequence is known for the U2 snRNA, the differences in migration may be due to developmentally-regulated base modifications. These snRNA variants may be involved in the alternative splicing of primary transcripts. The snRNA variants may produce functionally-different spliceosomes that splice primary transcripts in a developmental- or tissue-specific manner.","Made available in DSpace on 2011-05-07T13:53:49Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9136639.pdf: 6828224 bytes, checksum: 22cee2501b46590ed01f8956abf70114 (MD5) Previous issue date: 1991","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T15:00:29Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:28:37-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9136639","(UMI)AAI9136639","http://hdl.handle.net/2142/22857"],"dc:language":["eng"],"dc:rights":["Copyright 1991 Koehler, Richard Fred"],"dc:subject":["Biology, Molecular","Biology, Genetics"],"dc:title":["Developmental expression of a 4.5S RNA and variants of U1snRNA in Drosophila melanogaster"],"dc:type":["text"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:20Z"}