{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/22839"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/22839","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Formylmethanofuran formation in Methanobacterium thermoautotrophicum","abstract":"The initial step of methanogenesis from CO$\\sb2$ is the formation of formyl-methanofuran (formyl-MFR) from methanofuran, CO$\\sb2$ and H$\\sb2$. This reaction is not measurable in vitro unless activated. The enzymology, and the activation of this reaction were studied.","abstract_html":"The initial step of methanogenesis from CO$\\sb2$ is the formation of formyl-methanofuran (formyl-MFR) from methanofuran, CO$\\sb2$ and H$\\sb2$. This reaction is not measurable in vitro unless activated. The enzymology, and the activation of this reaction were studied.","abstract_has_math":true,"creators":["Bobik, Thomas Aquinas"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":["Wolfe, R.S."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T13:53:12Z","date_published":"2011-05-07T13:53:12Z","updated_at":"2026-07-22T22:25:20Z","subjects":["Biology, Microbiology"],"languages":["eng"],"rights":["Copyright 1990 Bobik, Thomas Aquinas"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9021651","(UMI)AAI9021651"],"render_values":[{"text":"AAI9021651","href":null,"code":true},{"text":"(UMI)AAI9021651","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/22839","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Wolfe, R.S."]},{"key":"dc:creator","label":"Author","values":["Bobik, Thomas Aquinas"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T13:53:12Z","10000-01-01","1990"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Microbiology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1990 Bobik, Thomas Aquinas"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9021651","(UMI)AAI9021651","http://hdl.handle.net/2142/22839"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The initial step of methanogenesis from CO$\\sb2$ is the formation of formyl-methanofuran (formyl-MFR) from methanofuran, CO$\\sb2$ and H$\\sb2$. This reaction is not measurable in vitro unless activated. The enzymology, and the activation of this reaction were studied.","It was found that the heterodisulfide of 2-mercaptoethanesulfonic acid and N-(7-mercaptoheptanoyl)threonine-O$\\sp3$-phosphate (CoM-S-S-HTP) was directly involved in the activation of formyl-MFR formation. Preliminary evidence indicated that CoM-S-S-HTP activated electron transfer to formyl-MFR formation; CoM-S-S-HTP was found to activate an unknown electron carrier present in cell extracts of Methanobacterium, and the low potential reducing agent titanium-(III)$\\cdot$citrate was found to bypass the CoM-S-S-HTP requirement of formyl-MFR synthesis. Additionally, CoM-S-S-HTP was shown to be a product of the methyl-reductase and the fumarate reductase reactions, and evidence indicated that each of these reactions activated formyl-MFR formation. Moreover, CoM-S-S-HTP was shown to be reduced by a heterodisulfide reductase and this reduction inactivated formyl-MFR formation.","Fractionation procedures were employed to resolve cell extract into two components each of which was required for formyl-MFR formation. One fraction (in the presence of CoM-S-S-HTP) reduced the redox dye metronidazole with electrons from H$\\sb2$, and the other fraction contained formyl-MFR dehydrogenase activity. Neither ATP nor a membrane fraction was required to reconstitute formyl-MFR formation, and the non-deazaflavin reducing hydrogenase was sufficient for formyl-MFR formation. It was proposed that the activation of formyl-MFR formation was used to coordinate the rate of CO$\\sb2$ fixation with the rates of methanogenesis and cell carbon synthesis.","Made available in DSpace on 2011-05-07T13:53:12Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9021651.pdf: 4519553 bytes, checksum: b9b9f5e44768996a3fb0ca1716ded5ad (MD5) Previous issue date: 1990","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T15:00:22Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:28:33-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Formylmethanofuran formation in Methanobacterium thermoautotrophicum"]}]}],"canonical_facts":{"dc:contributor":["Wolfe, R.S."],"dc:creator":["Bobik, Thomas Aquinas"],"dc:date":["2011-05-07T13:53:12Z","10000-01-01","1990"],"dc:description":["The initial step of methanogenesis from CO$\\sb2$ is the formation of formyl-methanofuran (formyl-MFR) from methanofuran, CO$\\sb2$ and H$\\sb2$. This reaction is not measurable in vitro unless activated. The enzymology, and the activation of this reaction were studied.","It was found that the heterodisulfide of 2-mercaptoethanesulfonic acid and N-(7-mercaptoheptanoyl)threonine-O$\\sp3$-phosphate (CoM-S-S-HTP) was directly involved in the activation of formyl-MFR formation. Preliminary evidence indicated that CoM-S-S-HTP activated electron transfer to formyl-MFR formation; CoM-S-S-HTP was found to activate an unknown electron carrier present in cell extracts of Methanobacterium, and the low potential reducing agent titanium-(III)$\\cdot$citrate was found to bypass the CoM-S-S-HTP requirement of formyl-MFR synthesis. Additionally, CoM-S-S-HTP was shown to be a product of the methyl-reductase and the fumarate reductase reactions, and evidence indicated that each of these reactions activated formyl-MFR formation. Moreover, CoM-S-S-HTP was shown to be reduced by a heterodisulfide reductase and this reduction inactivated formyl-MFR formation.","Fractionation procedures were employed to resolve cell extract into two components each of which was required for formyl-MFR formation. One fraction (in the presence of CoM-S-S-HTP) reduced the redox dye metronidazole with electrons from H$\\sb2$, and the other fraction contained formyl-MFR dehydrogenase activity. Neither ATP nor a membrane fraction was required to reconstitute formyl-MFR formation, and the non-deazaflavin reducing hydrogenase was sufficient for formyl-MFR formation. It was proposed that the activation of formyl-MFR formation was used to coordinate the rate of CO$\\sb2$ fixation with the rates of methanogenesis and cell carbon synthesis.","Made available in DSpace on 2011-05-07T13:53:12Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9021651.pdf: 4519553 bytes, checksum: b9b9f5e44768996a3fb0ca1716ded5ad (MD5) Previous issue date: 1990","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T15:00:22Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:28:33-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9021651","(UMI)AAI9021651","http://hdl.handle.net/2142/22839"],"dc:language":["eng"],"dc:rights":["Copyright 1990 Bobik, Thomas Aquinas"],"dc:subject":["Biology, Microbiology"],"dc:title":["Formylmethanofuran formation in Methanobacterium thermoautotrophicum"],"dc:type":["text"],"thesis:degree_discipline":["Microbiology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:20Z"}