{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/22804"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/22804","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"The role of basic fibroblast growth factor in the compensatory adrenal growth response","abstract":"We have investigated the role of basic fibroblast growth factor (bFGF) in the neurally mediated compensatory adrenal growth response. Unilateral adrenalectomy resulted in a 13%, 6% and 22% increase in adrenal weight, protein and DNA content, respectively, and 33-40% increases in the rate of cell proliferation measured by $\\sp3\\rm H$-thymidine incorporation in vitro. Three forms of bFGF, approximately 18.6, 21 and 22.5 kD were identified in rat adrenals by SDS-PAGE and Western blot. Basic FGF was localized immunocytochemically in cells of the glomerulosa and the medulla. Basic FGF stimulated a 68-80% increase in the rate of DNA synthesis in adrenal capsule-glomerulosa preps in vitro. Suramin (0.1 mM), a growth factor antagonist, blocked bFGF-receptor interaction in vitro and at 200 mg/kg given 5-7 days prior to adrenal surgery, blocked compensatory growth. Conversely, at 2.0 mg/kg, suramin significantly enhanced the compensatory growth response, perhaps caused by suramin-induced bFGF-receptor up-regulation as suramin-pretreatment also enhanced DNA synthesis in response to exogenous bFGF in vitro. Basic FGF receptors, with a $\\rm K\\sb{D}$ of 10 pM, were identified by binding analysis using primary cultures of rat glomerulosa cells, however, these results could not be demonstrated in capsule-glomerulosa tissue. Using autoradiography to verify the existence of bFGF receptors in vivo, heparin-insensitive $\\rm\\sp{125}I$-bFGF binding sites were concentrated primarily in the capsule and glomerulosa of the adrenal cortex suggesting the presence of FGF receptors. The presence of bFGF receptors was further verified by demonstration of internalization of $\\rm\\sp{125}I$-bFGF into cells of capsule-glomerulosa preps. This approach was used to demonstrate that suramin pretreatment of rats results in bFGF receptor up-regulation in the adrenal cortex. In addition we demonstrated a decrease in bFGF internalization in the remaining adrenal 24 hours after unilateral adrenalectomy suggesting the utilization of bFGF during the compensatory adrenal growth response. Together these data support the role for bFGF in autocrine stimulation of the adrenal cortex and in the compensatory adrenal growth response.","abstract_html":"We have investigated the role of basic fibroblast growth factor (bFGF) in the neurally mediated compensatory adrenal growth response. Unilateral adrenalectomy resulted in a 13%, 6% and 22% increase in adrenal weight, protein and DNA content, respectively, and 33-40% increases in the rate of cell proliferation measured by $\\sp3\\rm H$-thymidine incorporation in vitro. Three forms of bFGF, approximately 18.6, 21 and 22.5 kD were identified in rat adrenals by SDS-PAGE and Western blot. Basic FGF was localized immunocytochemically in cells of the glomerulosa and the medulla. Basic FGF stimulated a 68-80% increase in the rate of DNA synthesis in adrenal capsule-glomerulosa preps in vitro. Suramin (0.1 mM), a growth factor antagonist, blocked bFGF-receptor interaction in vitro and at 200 mg/kg given 5-7 days prior to adrenal surgery, blocked compensatory growth. Conversely, at 2.0 mg/kg, suramin significantly enhanced the compensatory growth response, perhaps caused by suramin-induced bFGF-receptor up-regulation as suramin-pretreatment also enhanced DNA synthesis in response to exogenous bFGF in vitro. Basic FGF receptors, with a $\\rm K\\sb{D}$ of 10 pM, were identified by binding analysis using primary cultures of rat glomerulosa cells, however, these results could not be demonstrated in capsule-glomerulosa tissue. Using autoradiography to verify the existence of bFGF receptors in vivo, heparin-insensitive $\\rm\\sp{125}I$-bFGF binding sites were concentrated primarily in the capsule and glomerulosa of the adrenal cortex suggesting the presence of FGF receptors. The presence of bFGF receptors was further verified by demonstration of internalization of $\\rm\\sp{125}I$-bFGF into cells of capsule-glomerulosa preps. This approach was used to demonstrate that suramin pretreatment of rats results in bFGF receptor up-regulation in the adrenal cortex. In addition we demonstrated a decrease in bFGF internalization in the remaining adrenal 24 hours after unilateral adrenalectomy suggesting the utilization of bFGF during the compensatory adrenal growth response. Together these data support the role for bFGF in autocrine stimulation of the adrenal cortex and in the compensatory adrenal growth response.","abstract_has_math":true,"creators":["Basile, David Patrick"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Molecular and Integrative Physiology","degree_department":null,"school":null,"contributors":["Holzwarth, Matilde"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T13:52:02Z","date_published":"2011-05-07T13:52:02Z","updated_at":"2026-07-22T22:25:20Z","subjects":["Biology, Animal Physiology"],"languages":["eng"],"rights":["Copyright 1994 Basile, David Patrick"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9503137","(UMI)AAI9503137"],"render_values":[{"text":"AAI9503137","href":null,"code":true},{"text":"(UMI)AAI9503137","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/22804","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Holzwarth, Matilde"]},{"key":"dc:creator","label":"Author","values":["Basile, David Patrick"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T13:52:02Z","10000-01-01","1994"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Molecular and Integrative Physiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Animal Physiology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1994 Basile, David Patrick"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9503137","(UMI)AAI9503137","http://hdl.handle.net/2142/22804"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["We have investigated the role of basic fibroblast growth factor (bFGF) in the neurally mediated compensatory adrenal growth response. Unilateral adrenalectomy resulted in a 13%, 6% and 22% increase in adrenal weight, protein and DNA content, respectively, and 33-40% increases in the rate of cell proliferation measured by $\\sp3\\rm H$-thymidine incorporation in vitro. Three forms of bFGF, approximately 18.6, 21 and 22.5 kD were identified in rat adrenals by SDS-PAGE and Western blot. Basic FGF was localized immunocytochemically in cells of the glomerulosa and the medulla. Basic FGF stimulated a 68-80% increase in the rate of DNA synthesis in adrenal capsule-glomerulosa preps in vitro. Suramin (0.1 mM), a growth factor antagonist, blocked bFGF-receptor interaction in vitro and at 200 mg/kg given 5-7 days prior to adrenal surgery, blocked compensatory growth. Conversely, at 2.0 mg/kg, suramin significantly enhanced the compensatory growth response, perhaps caused by suramin-induced bFGF-receptor up-regulation as suramin-pretreatment also enhanced DNA synthesis in response to exogenous bFGF in vitro. Basic FGF receptors, with a $\\rm K\\sb{D}$ of 10 pM, were identified by binding analysis using primary cultures of rat glomerulosa cells, however, these results could not be demonstrated in capsule-glomerulosa tissue. Using autoradiography to verify the existence of bFGF receptors in vivo, heparin-insensitive $\\rm\\sp{125}I$-bFGF binding sites were concentrated primarily in the capsule and glomerulosa of the adrenal cortex suggesting the presence of FGF receptors. The presence of bFGF receptors was further verified by demonstration of internalization of $\\rm\\sp{125}I$-bFGF into cells of capsule-glomerulosa preps. This approach was used to demonstrate that suramin pretreatment of rats results in bFGF receptor up-regulation in the adrenal cortex. In addition we demonstrated a decrease in bFGF internalization in the remaining adrenal 24 hours after unilateral adrenalectomy suggesting the utilization of bFGF during the compensatory adrenal growth response. Together these data support the role for bFGF in autocrine stimulation of the adrenal cortex and in the compensatory adrenal growth response.","Made available in DSpace on 2011-05-07T13:52:02Z (GMT). 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Unilateral adrenalectomy resulted in a 13%, 6% and 22% increase in adrenal weight, protein and DNA content, respectively, and 33-40% increases in the rate of cell proliferation measured by $\\sp3\\rm H$-thymidine incorporation in vitro. Three forms of bFGF, approximately 18.6, 21 and 22.5 kD were identified in rat adrenals by SDS-PAGE and Western blot. Basic FGF was localized immunocytochemically in cells of the glomerulosa and the medulla. Basic FGF stimulated a 68-80% increase in the rate of DNA synthesis in adrenal capsule-glomerulosa preps in vitro. Suramin (0.1 mM), a growth factor antagonist, blocked bFGF-receptor interaction in vitro and at 200 mg/kg given 5-7 days prior to adrenal surgery, blocked compensatory growth. Conversely, at 2.0 mg/kg, suramin significantly enhanced the compensatory growth response, perhaps caused by suramin-induced bFGF-receptor up-regulation as suramin-pretreatment also enhanced DNA synthesis in response to exogenous bFGF in vitro. Basic FGF receptors, with a $\\rm K\\sb{D}$ of 10 pM, were identified by binding analysis using primary cultures of rat glomerulosa cells, however, these results could not be demonstrated in capsule-glomerulosa tissue. Using autoradiography to verify the existence of bFGF receptors in vivo, heparin-insensitive $\\rm\\sp{125}I$-bFGF binding sites were concentrated primarily in the capsule and glomerulosa of the adrenal cortex suggesting the presence of FGF receptors. The presence of bFGF receptors was further verified by demonstration of internalization of $\\rm\\sp{125}I$-bFGF into cells of capsule-glomerulosa preps. This approach was used to demonstrate that suramin pretreatment of rats results in bFGF receptor up-regulation in the adrenal cortex. In addition we demonstrated a decrease in bFGF internalization in the remaining adrenal 24 hours after unilateral adrenalectomy suggesting the utilization of bFGF during the compensatory adrenal growth response. Together these data support the role for bFGF in autocrine stimulation of the adrenal cortex and in the compensatory adrenal growth response.","Made available in DSpace on 2011-05-07T13:52:02Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9503137.pdf: 5367908 bytes, checksum: f10d43febed90cad69aa1e627102fe8c (MD5) Previous issue date: 1994","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T15:00:07Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:28:25-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9503137","(UMI)AAI9503137","http://hdl.handle.net/2142/22804"],"dc:language":["eng"],"dc:rights":["Copyright 1994 Basile, David Patrick"],"dc:subject":["Biology, Animal Physiology"],"dc:title":["The role of basic fibroblast growth factor in the compensatory adrenal growth response"],"dc:type":["text"],"thesis:degree_discipline":["Molecular and Integrative Physiology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:20Z"}