{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/22783"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/22783","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"The structure of cyclic fatty acid monomers isolated from heated linseed oil and their effects on cultured aortic endothelial cells","abstract":"This study reports the complete structural configuration of CFAM isolated from heated linseed oil and the influence of these components in cultured porcine aortic endothelial cells. Using CFAM-oxazoline derivatives, location of unsaturation and ring structures were confirmed using gas chromatography-matrix isolation-Fourier transform infrared spectroscopy and gas chromatography-mass spectrometry. The CFAM were identified as 18-carbon diunsaturated structures containing cyclopentenyl and cyclohexenyl rings in various positions along the molecule. One site of cis unsaturation was always within the ring structure while the other site of unsaturation was either cis or trans and could be located at various positions in either the carboxyl- or methyl-terminal chain of the ring. Three pairs were identified as cyclic stereoisomers.","abstract_html":"This study reports the complete structural configuration of CFAM isolated from heated linseed oil and the influence of these components in cultured porcine aortic endothelial cells. Using CFAM-oxazoline derivatives, location of unsaturation and ring structures were confirmed using gas chromatography-matrix isolation-Fourier transform infrared spectroscopy and gas chromatography-mass spectrometry. The CFAM were identified as 18-carbon diunsaturated structures containing cyclopentenyl and cyclohexenyl rings in various positions along the molecule. One site of cis unsaturation was always within the ring structure while the other site of unsaturation was either cis or trans and could be located at various positions in either the carboxyl- or methyl-terminal chain of the ring. Three pairs were identified as cyclic stereoisomers.","abstract_has_math":false,"creators":["Flickinger, Brent David"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Food Science and Human Nutrition","degree_department":null,"school":null,"contributors":["Perkins, Edward G."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T13:51:21Z","date_published":"2011-05-07T13:51:21Z","updated_at":"2026-07-22T22:25:20Z","subjects":["Biology, Cell","Biology, Animal Physiology","Health Sciences, Nutrition"],"languages":["eng"],"rights":["Copyright 1995 Flickinger, Brent David"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9543585","(UMI)AAI9543585"],"render_values":[{"text":"AAI9543585","href":null,"code":true},{"text":"(UMI)AAI9543585","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/22783","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Perkins, Edward G."]},{"key":"dc:creator","label":"Author","values":["Flickinger, Brent David"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T13:51:21Z","10000-01-01","1995"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Food Science and Human Nutrition"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Cell","Biology, Animal Physiology","Health Sciences, Nutrition"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1995 Flickinger, Brent David"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9543585","(UMI)AAI9543585","http://hdl.handle.net/2142/22783"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["This study reports the complete structural configuration of CFAM isolated from heated linseed oil and the influence of these components in cultured porcine aortic endothelial cells. Using CFAM-oxazoline derivatives, location of unsaturation and ring structures were confirmed using gas chromatography-matrix isolation-Fourier transform infrared spectroscopy and gas chromatography-mass spectrometry. The CFAM were identified as 18-carbon diunsaturated structures containing cyclopentenyl and cyclohexenyl rings in various positions along the molecule. One site of cis unsaturation was always within the ring structure while the other site of unsaturation was either cis or trans and could be located at various positions in either the carboxyl- or methyl-terminal chain of the ring. Three pairs were identified as cyclic stereoisomers.","After incubation with CFAM-containing media for 48 hours, CFAM were incorporated into the membrane phospholipids of cultured endothelial cells. No decrease in endothelial cell density or viability was obsessed. A significant decrease in the activity of calcium ATPase as well as a significant decrease in the integrity of the endothelial cell monolayer were observed following incubation with CFAM-containing media. A decrease in the activity of total ATPase also appeared to be caused by incubation with CFAM.","The incubation of CFAM-containing media caused an increase in the fluidity of the membrane hydrocarbon region as measured by the steady-state anisotropy of DPH. This observed fluidity change may be an artifact caused by DPH incorporation into the intracellular lipid droplets present in CFAM-treated cells. The measurements of the steady-state anisotropy of both TMA-DPH and DPH-PA showed no change in the membrane fluidity.","Endothelial cell growth was shown to be influenced by the presence of fatty-acid free bovine serum albumin, which was used to introduce CFAM into the culture media, and not due to the incorporation of CFAM into endothelial cell membranes. Prostacyclin production was significantly increased in endothelial cells due to incubation with CFAM-BSA compared to BSA alone. The presence of CFAM was either counteracting the inhibitory influence of BSA on PGI$\\sb2$ production or directly stimulating PGI$\\sb2$ secretion by cultured endothelial cells.","Made available in DSpace on 2011-05-07T13:51:21Z (GMT). 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Using CFAM-oxazoline derivatives, location of unsaturation and ring structures were confirmed using gas chromatography-matrix isolation-Fourier transform infrared spectroscopy and gas chromatography-mass spectrometry. The CFAM were identified as 18-carbon diunsaturated structures containing cyclopentenyl and cyclohexenyl rings in various positions along the molecule. One site of cis unsaturation was always within the ring structure while the other site of unsaturation was either cis or trans and could be located at various positions in either the carboxyl- or methyl-terminal chain of the ring. Three pairs were identified as cyclic stereoisomers.","After incubation with CFAM-containing media for 48 hours, CFAM were incorporated into the membrane phospholipids of cultured endothelial cells. No decrease in endothelial cell density or viability was obsessed. A significant decrease in the activity of calcium ATPase as well as a significant decrease in the integrity of the endothelial cell monolayer were observed following incubation with CFAM-containing media. A decrease in the activity of total ATPase also appeared to be caused by incubation with CFAM.","The incubation of CFAM-containing media caused an increase in the fluidity of the membrane hydrocarbon region as measured by the steady-state anisotropy of DPH. This observed fluidity change may be an artifact caused by DPH incorporation into the intracellular lipid droplets present in CFAM-treated cells. The measurements of the steady-state anisotropy of both TMA-DPH and DPH-PA showed no change in the membrane fluidity.","Endothelial cell growth was shown to be influenced by the presence of fatty-acid free bovine serum albumin, which was used to introduce CFAM into the culture media, and not due to the incorporation of CFAM into endothelial cell membranes. Prostacyclin production was significantly increased in endothelial cells due to incubation with CFAM-BSA compared to BSA alone. The presence of CFAM was either counteracting the inhibitory influence of BSA on PGI$\\sb2$ production or directly stimulating PGI$\\sb2$ secretion by cultured endothelial cells.","Made available in DSpace on 2011-05-07T13:51:21Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9543585.pdf: 5442763 bytes, checksum: ad1928c4194e42aac298fd7ea5f3d933 (MD5) Previous issue date: 1995","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:59:58Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:28:20-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9543585","(UMI)AAI9543585","http://hdl.handle.net/2142/22783"],"dc:language":["eng"],"dc:rights":["Copyright 1995 Flickinger, Brent David"],"dc:subject":["Biology, Cell","Biology, Animal Physiology","Health Sciences, Nutrition"],"dc:title":["The structure of cyclic fatty acid monomers isolated from heated linseed oil and their effects on cultured aortic endothelial cells"],"dc:type":["text"],"thesis:degree_discipline":["Food Science and Human Nutrition"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:20Z"}