{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/22537"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/22537","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Molecular characterization and induction of a peach beta-1,3-glucanase gene","abstract":"A peach genomic library of cultivar Stark Earligo was screened using a pea $\\beta$-1,3-glucanase gene as a probe. Five clones containing $\\beta$-1,3-glucanase sequence were isolated and characterized. A 3.85 Kb DNA fragment of one of the genomic clones, containing peach $\\beta$-1,3-glucanase gene was sequenced in both the directions. The peach $\\beta$-1,3-glucanase gene, PpGns1, encodes a proenzyme of 350 amino acids, which consists of a 38 amino acid signal peptide and a 312 amino acid mature peptide. This is the longest signal peptide of all plant $\\beta$-1,3-glucanase genes so far reported. Putative TATA and CAAT boxes in the 5$\\prime$ flanking region and two polyadenylation signals in the 3$\\prime$ flanking region were identified. The PpGns1 was induced in in vitro-grown peach shoot cultures following treatment with a culture filtrate of Xanthomonas campestris pv. pruni and ethephon within 12-18 h. However, it was not induced following treatment with mercuric chloride. Characterization of four additional clones revealed two other different types of $\\beta$-1,3-glucanase genes in peach. Southern blot analysis of total genomic DNA also indicated that $\\beta$-1,3-glucanase gene belongs to a multigene family in peach.","abstract_html":"A peach genomic library of cultivar Stark Earligo was screened using a pea <span class=\"etd-inline-math\">&beta;</span>-1,3-glucanase gene as a probe. Five clones containing <span class=\"etd-inline-math\">&beta;</span>-1,3-glucanase sequence were isolated and characterized. A 3.85 Kb DNA fragment of one of the genomic clones, containing peach <span class=\"etd-inline-math\">&beta;</span>-1,3-glucanase gene was sequenced in both the directions. The peach <span class=\"etd-inline-math\">&beta;</span>-1,3-glucanase gene, PpGns1, encodes a proenzyme of 350 amino acids, which consists of a 38 amino acid signal peptide and a 312 amino acid mature peptide. This is the longest signal peptide of all plant <span class=\"etd-inline-math\">&beta;</span>-1,3-glucanase genes so far reported. Putative TATA and CAAT boxes in the 5$\\prime$ flanking region and two polyadenylation signals in the 3$\\prime$ flanking region were identified. The PpGns1 was induced in in vitro-grown peach shoot cultures following treatment with a culture filtrate of Xanthomonas campestris pv. pruni and ethephon within 12-18 h. However, it was not induced following treatment with mercuric chloride. Characterization of four additional clones revealed two other different types of <span class=\"etd-inline-math\">&beta;</span>-1,3-glucanase genes in peach. Southern blot analysis of total genomic DNA also indicated that <span class=\"etd-inline-math\">&beta;</span>-1,3-glucanase gene belongs to a multigene family in peach.","abstract_has_math":true,"creators":["Thimmapuram, Jyothi"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Korban, Schuyler S."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T13:43:01Z","date_published":"2011-05-07T13:43:01Z","updated_at":"2026-07-22T22:25:20Z","subjects":["Biology, Molecular","Biology, Plant Physiology"],"languages":["eng"],"rights":["Copyright 1996 Thimmapuram, Jyothi"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9625202","(UMI)AAI9625202"],"render_values":[{"text":"AAI9625202","href":null,"code":true},{"text":"(UMI)AAI9625202","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/22537","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Korban, Schuyler S."]},{"key":"dc:creator","label":"Author","values":["Thimmapuram, Jyothi"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T13:43:01Z","10000-01-01","1996"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Molecular","Biology, Plant Physiology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1996 Thimmapuram, Jyothi"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9625202","(UMI)AAI9625202","http://hdl.handle.net/2142/22537"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["A peach genomic library of cultivar Stark Earligo was screened using a pea $\\beta$-1,3-glucanase gene as a probe. Five clones containing $\\beta$-1,3-glucanase sequence were isolated and characterized. A 3.85 Kb DNA fragment of one of the genomic clones, containing peach $\\beta$-1,3-glucanase gene was sequenced in both the directions. The peach $\\beta$-1,3-glucanase gene, PpGns1, encodes a proenzyme of 350 amino acids, which consists of a 38 amino acid signal peptide and a 312 amino acid mature peptide. This is the longest signal peptide of all plant $\\beta$-1,3-glucanase genes so far reported. Putative TATA and CAAT boxes in the 5$\\prime$ flanking region and two polyadenylation signals in the 3$\\prime$ flanking region were identified. The PpGns1 was induced in in vitro-grown peach shoot cultures following treatment with a culture filtrate of Xanthomonas campestris pv. pruni and ethephon within 12-18 h. However, it was not induced following treatment with mercuric chloride. Characterization of four additional clones revealed two other different types of $\\beta$-1,3-glucanase genes in peach. Southern blot analysis of total genomic DNA also indicated that $\\beta$-1,3-glucanase gene belongs to a multigene family in peach.","Made available in DSpace on 2011-05-07T13:43:01Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9625202.pdf: 3836124 bytes, checksum: cffdbd039923ae56e1b8d16a2ffdef9f (MD5) Previous issue date: 1996","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:58:18Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:27:24-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Molecular characterization and induction of a peach beta-1,3-glucanase gene"]}]}],"canonical_facts":{"dc:contributor":["Korban, Schuyler S."],"dc:creator":["Thimmapuram, Jyothi"],"dc:date":["2011-05-07T13:43:01Z","10000-01-01","1996"],"dc:description":["A peach genomic library of cultivar Stark Earligo was screened using a pea $\\beta$-1,3-glucanase gene as a probe. Five clones containing $\\beta$-1,3-glucanase sequence were isolated and characterized. A 3.85 Kb DNA fragment of one of the genomic clones, containing peach $\\beta$-1,3-glucanase gene was sequenced in both the directions. The peach $\\beta$-1,3-glucanase gene, PpGns1, encodes a proenzyme of 350 amino acids, which consists of a 38 amino acid signal peptide and a 312 amino acid mature peptide. This is the longest signal peptide of all plant $\\beta$-1,3-glucanase genes so far reported. Putative TATA and CAAT boxes in the 5$\\prime$ flanking region and two polyadenylation signals in the 3$\\prime$ flanking region were identified. The PpGns1 was induced in in vitro-grown peach shoot cultures following treatment with a culture filtrate of Xanthomonas campestris pv. pruni and ethephon within 12-18 h. However, it was not induced following treatment with mercuric chloride. Characterization of four additional clones revealed two other different types of $\\beta$-1,3-glucanase genes in peach. Southern blot analysis of total genomic DNA also indicated that $\\beta$-1,3-glucanase gene belongs to a multigene family in peach.","Made available in DSpace on 2011-05-07T13:43:01Z (GMT). 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