{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/22441"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/22441","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Effects of food restriction and lysine supplementation on protein metabolism in the rat","abstract":"These studies examined the effects of prolonged food restriction and lysine supplementation on growth, muscle mass, and protein metabolism in the rat. Two levels of food restriction (40% and 75%) were utilized during an 8-week feeding period to produce moderate or severe undernutrition. Rats restricted to 75% of ad libitum intake lost weight, but reached a stable body weight during the last 4 weeks. The stable body weight was achieved when the animals reached an energy intake/unit body weight comparable to ad libitum fed, age controls.","abstract_html":"These studies examined the effects of prolonged food restriction and lysine supplementation on growth, muscle mass, and protein metabolism in the rat. Two levels of food restriction (40% and 75%) were utilized during an 8-week feeding period to produce moderate or severe undernutrition. Rats restricted to 75% of ad libitum intake lost weight, but reached a stable body weight during the last 4 weeks. The stable body weight was achieved when the animals reached an energy intake/unit body weight comparable to ad libitum fed, age controls.","abstract_has_math":false,"creators":["Sika, Mohammed"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Health Sciences, Nutrition","degree_department":null,"school":null,"contributors":["Layman, Donald K."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T13:39:56Z","date_published":"2011-05-07T13:39:56Z","updated_at":"2026-07-22T22:25:20Z","subjects":["Health Sciences, Nutrition"],"languages":["eng"],"rights":["Copyright 1991 Sika, Mohammed"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9124489","(UMI)AAI9124489"],"render_values":[{"text":"AAI9124489","href":null,"code":true},{"text":"(UMI)AAI9124489","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/22441","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Layman, Donald K."]},{"key":"dc:creator","label":"Author","values":["Sika, Mohammed"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T13:39:56Z","10000-01-01","1991"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Health Sciences, Nutrition"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Health Sciences, Nutrition"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1991 Sika, Mohammed"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9124489","(UMI)AAI9124489","http://hdl.handle.net/2142/22441"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["These studies examined the effects of prolonged food restriction and lysine supplementation on growth, muscle mass, and protein metabolism in the rat. Two levels of food restriction (40% and 75%) were utilized during an 8-week feeding period to produce moderate or severe undernutrition. Rats restricted to 75% of ad libitum intake lost weight, but reached a stable body weight during the last 4 weeks. The stable body weight was achieved when the animals reached an energy intake/unit body weight comparable to ad libitum fed, age controls.","Lysine supplementation of the wheat gluten diet produced a significant increase in growth of muscle mass in rats fed ad libitum or at the 40% restricted level. Urinary 3-methylhistidine was increased by lysine supplementation in 75% restricted rats suggesting an increase in myofibrillar protein breakdown.","Changes in whole body and muscle protein turnover were investigated in animals after 8 weeks on the respective diets. Measurements were made in both fed (absorptive) and fasted (postabsorptive) states to evaluate the significance of diurnal variations. In the fed state, restricted rats had lower or the same fractional synthesis rates (FSR) of muscle protein as the ad libitum fed rats. However, in the fasted state, restricted rats had a higher skeletal muscle FSR than ad libitum fed rats. Whole body protein turnover, expressed per unit of body weight, was the same in ad libitum and food restricted groups.","In the fed state, lysine supplementation increased FSR for muscles in ad libitum and 40% restricted rats. However, FSR in skeletal muscles decreased in the 75% restricted rats. In the fasted state, lysine supplementation resulted in a decrease in muscle FSR's.","This study demonstrates that rats can reach and maintain a smaller body weight during prolonged undernutrition. This stable body weight is characterized by nitrogen balance and similar rates of whole body protein turnover per unit of body weight as ad libitum fed rats. These studies also showed that the threshold for beneficial effects of lysine supplementation is somewhere between 40% and 75% food restriction.","Made available in DSpace on 2011-05-07T13:39:56Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9124489.pdf: 4806125 bytes, checksum: 4fcb50045cb436eb964291b0a5844de7 (MD5) Previous issue date: 1991","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:57:38Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:27:02-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Effects of food restriction and lysine supplementation on protein metabolism in the rat"]}]}],"canonical_facts":{"dc:contributor":["Layman, Donald K."],"dc:creator":["Sika, Mohammed"],"dc:date":["2011-05-07T13:39:56Z","10000-01-01","1991"],"dc:description":["These studies examined the effects of prolonged food restriction and lysine supplementation on growth, muscle mass, and protein metabolism in the rat. Two levels of food restriction (40% and 75%) were utilized during an 8-week feeding period to produce moderate or severe undernutrition. Rats restricted to 75% of ad libitum intake lost weight, but reached a stable body weight during the last 4 weeks. The stable body weight was achieved when the animals reached an energy intake/unit body weight comparable to ad libitum fed, age controls.","Lysine supplementation of the wheat gluten diet produced a significant increase in growth of muscle mass in rats fed ad libitum or at the 40% restricted level. Urinary 3-methylhistidine was increased by lysine supplementation in 75% restricted rats suggesting an increase in myofibrillar protein breakdown.","Changes in whole body and muscle protein turnover were investigated in animals after 8 weeks on the respective diets. Measurements were made in both fed (absorptive) and fasted (postabsorptive) states to evaluate the significance of diurnal variations. In the fed state, restricted rats had lower or the same fractional synthesis rates (FSR) of muscle protein as the ad libitum fed rats. However, in the fasted state, restricted rats had a higher skeletal muscle FSR than ad libitum fed rats. Whole body protein turnover, expressed per unit of body weight, was the same in ad libitum and food restricted groups.","In the fed state, lysine supplementation increased FSR for muscles in ad libitum and 40% restricted rats. However, FSR in skeletal muscles decreased in the 75% restricted rats. In the fasted state, lysine supplementation resulted in a decrease in muscle FSR's.","This study demonstrates that rats can reach and maintain a smaller body weight during prolonged undernutrition. This stable body weight is characterized by nitrogen balance and similar rates of whole body protein turnover per unit of body weight as ad libitum fed rats. These studies also showed that the threshold for beneficial effects of lysine supplementation is somewhere between 40% and 75% food restriction.","Made available in DSpace on 2011-05-07T13:39:56Z (GMT). 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