{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/22418"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/22418","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Molecular analysis of thecDNA and gene for a major protein from flexible cuticles of the giant silkmoth Hyalophora cecropia","abstract":"HCCP12 is a major protein found in flexible cuticles of all three metamorphic stages of the giant silkmoth Hyalophora cecropia. The distribution of HCCP12 provides an opportunity to study the differential regulation of gene expression since there are persistent populations of epidermal cells that secrete the cuticular proteins during metamorphosis. An oligonucleotide probe, designed from N-terminal protein sequence, was used to screen a cDNA library made using mRNA of larval abdominal epidermal cells. A cDNA clone (cDNA1) was shown to encode HCCP12 by sequencing and Northern analysis. cDNA1 was used to screen a H. cecropia genomic library, and the clone II-5 was shown to contain the gene for HCCP12 by sequencing of the subclone pT7-2.8HE. This subclone contained the only fragment of II-5 that hybridized to cDNA1, indicating that HCCP12 is the only related cuticular protein gene in the 17 Kb genomic region. A set of ten GTCT repeats was found in the 5$\\sp\\prime$ sequence flanking the coding region for HCCP12. Searches of the GenBank database revealed that a number of other sequences contain similar repeat regions, but none of the repeats have been shown to have any functional significance. Sequence analysis by RACE (rapid amplification of cDNA ends) of the 5$\\sp\\prime$ ends of mRNAs for HCCP12 isolated from larval, pupal, and pharate adult epidermal cells was used to determine the transcriptional start site, and showed that cDNA 1 is ten bases short of full length. Furthermore, it was concluded that a single gene and a single promoter are used in the three metamorphic stages since the 5$\\sp\\prime$ ends of the mRNAs from the various tissues were identical. Thus, metamorphosis does not require stage-specific gene sets or promoters.","abstract_html":"HCCP12 is a major protein found in flexible cuticles of all three metamorphic stages of the giant silkmoth Hyalophora cecropia. The distribution of HCCP12 provides an opportunity to study the differential regulation of gene expression since there are persistent populations of epidermal cells that secrete the cuticular proteins during metamorphosis. An oligonucleotide probe, designed from N-terminal protein sequence, was used to screen a cDNA library made using mRNA of larval abdominal epidermal cells. A cDNA clone (cDNA1) was shown to encode HCCP12 by sequencing and Northern analysis. cDNA1 was used to screen a H. cecropia genomic library, and the clone II-5 was shown to contain the gene for HCCP12 by sequencing of the subclone pT7-2.8HE. This subclone contained the only fragment of II-5 that hybridized to cDNA1, indicating that HCCP12 is the only related cuticular protein gene in the 17 Kb genomic region. A set of ten GTCT repeats was found in the 5$\\sp\\prime$ sequence flanking the coding region for HCCP12. Searches of the GenBank database revealed that a number of other sequences contain similar repeat regions, but none of the repeats have been shown to have any functional significance. Sequence analysis by RACE (rapid amplification of cDNA ends) of the 5$\\sp\\prime$ ends of mRNAs for HCCP12 isolated from larval, pupal, and pharate adult epidermal cells was used to determine the transcriptional start site, and showed that cDNA 1 is ten bases short of full length. Furthermore, it was concluded that a single gene and a single promoter are used in the three metamorphic stages since the 5$\\sp\\prime$ ends of the mRNAs from the various tissues were identical. Thus, metamorphosis does not require stage-specific gene sets or promoters.","abstract_has_math":true,"creators":["Binger, Lynetta C."],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Entomology","degree_department":null,"school":null,"contributors":["Willis, Judith H."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T13:39:12Z","date_published":"2011-05-07T13:39:12Z","updated_at":"2026-07-22T22:25:19Z","subjects":["Biology, Entomology","Biology, Genetics","Biology, Cell"],"languages":["eng"],"rights":["Copyright 1991 Binger, Lynetta C."],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9210746","(UMI)AAI9210746"],"render_values":[{"text":"AAI9210746","href":null,"code":true},{"text":"(UMI)AAI9210746","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/22418","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Willis, Judith H."]},{"key":"dc:creator","label":"Author","values":["Binger, Lynetta C."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T13:39:12Z","10000-01-01","1991"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Entomology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Entomology","Biology, Genetics","Biology, Cell"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1991 Binger, Lynetta C."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9210746","(UMI)AAI9210746","http://hdl.handle.net/2142/22418"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["HCCP12 is a major protein found in flexible cuticles of all three metamorphic stages of the giant silkmoth Hyalophora cecropia. The distribution of HCCP12 provides an opportunity to study the differential regulation of gene expression since there are persistent populations of epidermal cells that secrete the cuticular proteins during metamorphosis. An oligonucleotide probe, designed from N-terminal protein sequence, was used to screen a cDNA library made using mRNA of larval abdominal epidermal cells. A cDNA clone (cDNA1) was shown to encode HCCP12 by sequencing and Northern analysis. cDNA1 was used to screen a H. cecropia genomic library, and the clone II-5 was shown to contain the gene for HCCP12 by sequencing of the subclone pT7-2.8HE. This subclone contained the only fragment of II-5 that hybridized to cDNA1, indicating that HCCP12 is the only related cuticular protein gene in the 17 Kb genomic region. A set of ten GTCT repeats was found in the 5$\\sp\\prime$ sequence flanking the coding region for HCCP12. Searches of the GenBank database revealed that a number of other sequences contain similar repeat regions, but none of the repeats have been shown to have any functional significance. Sequence analysis by RACE (rapid amplification of cDNA ends) of the 5$\\sp\\prime$ ends of mRNAs for HCCP12 isolated from larval, pupal, and pharate adult epidermal cells was used to determine the transcriptional start site, and showed that cDNA 1 is ten bases short of full length. Furthermore, it was concluded that a single gene and a single promoter are used in the three metamorphic stages since the 5$\\sp\\prime$ ends of the mRNAs from the various tissues were identical. Thus, metamorphosis does not require stage-specific gene sets or promoters.","Made available in DSpace on 2011-05-07T13:39:12Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9210746.pdf: 2791333 bytes, checksum: 025df949dae9160faa453764616b592f (MD5) Previous issue date: 1991","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:57:29Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:26:58-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Molecular analysis of thecDNA and gene for a major protein from flexible cuticles of the giant silkmoth Hyalophora cecropia"]}]}],"canonical_facts":{"dc:contributor":["Willis, Judith H."],"dc:creator":["Binger, Lynetta C."],"dc:date":["2011-05-07T13:39:12Z","10000-01-01","1991"],"dc:description":["HCCP12 is a major protein found in flexible cuticles of all three metamorphic stages of the giant silkmoth Hyalophora cecropia. The distribution of HCCP12 provides an opportunity to study the differential regulation of gene expression since there are persistent populations of epidermal cells that secrete the cuticular proteins during metamorphosis. An oligonucleotide probe, designed from N-terminal protein sequence, was used to screen a cDNA library made using mRNA of larval abdominal epidermal cells. A cDNA clone (cDNA1) was shown to encode HCCP12 by sequencing and Northern analysis. cDNA1 was used to screen a H. cecropia genomic library, and the clone II-5 was shown to contain the gene for HCCP12 by sequencing of the subclone pT7-2.8HE. This subclone contained the only fragment of II-5 that hybridized to cDNA1, indicating that HCCP12 is the only related cuticular protein gene in the 17 Kb genomic region. A set of ten GTCT repeats was found in the 5$\\sp\\prime$ sequence flanking the coding region for HCCP12. Searches of the GenBank database revealed that a number of other sequences contain similar repeat regions, but none of the repeats have been shown to have any functional significance. Sequence analysis by RACE (rapid amplification of cDNA ends) of the 5$\\sp\\prime$ ends of mRNAs for HCCP12 isolated from larval, pupal, and pharate adult epidermal cells was used to determine the transcriptional start site, and showed that cDNA 1 is ten bases short of full length. Furthermore, it was concluded that a single gene and a single promoter are used in the three metamorphic stages since the 5$\\sp\\prime$ ends of the mRNAs from the various tissues were identical. Thus, metamorphosis does not require stage-specific gene sets or promoters.","Made available in DSpace on 2011-05-07T13:39:12Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9210746.pdf: 2791333 bytes, checksum: 025df949dae9160faa453764616b592f (MD5) Previous issue date: 1991","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:57:29Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:26:58-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9210746","(UMI)AAI9210746","http://hdl.handle.net/2142/22418"],"dc:language":["eng"],"dc:rights":["Copyright 1991 Binger, Lynetta C."],"dc:subject":["Biology, Entomology","Biology, Genetics","Biology, Cell"],"dc:title":["Molecular analysis of thecDNA and gene for a major protein from flexible cuticles of the giant silkmoth Hyalophora cecropia"],"dc:type":["text"],"thesis:degree_discipline":["Entomology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:19Z"}