{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/21758"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/21758","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Enzyme-linked immunosorbent assay for herbicide detection","abstract":"The purpose of this research was to develop polyclonal antibodies to the soybean herbicides clomazone (2- ((2 chlorophenyl)methyl) -4,4-dimethyl-3-isoxazolidinone), imazaquin (2- (4,5-dihydro-4-methyl-4-(1-methylethyl)-5-oxo-1H-imidazol-2-yl) -3-quinolinecarboxylic acid), and metribuzin (4-amino-6-(1,1-dimethylethyl)-3-(methylthio)-1,2,4-triazin-5(4H)-one). The herbicide-specific antibodies were to be used in an enzyme linked immunosorbent assay (ELISA) for herbicide detection in soil.","abstract_html":"The purpose of this research was to develop polyclonal antibodies to the soybean herbicides clomazone (2- ((2 chlorophenyl)methyl) -4,4-dimethyl-3-isoxazolidinone), imazaquin (2- (4,5-dihydro-4-methyl-4-(1-methylethyl)-5-oxo-1H-imidazol-2-yl) -3-quinolinecarboxylic acid), and metribuzin (4-amino-6-(1,1-dimethylethyl)-3-(methylthio)-1,2,4-triazin-5(4H)-one). The herbicide-specific antibodies were to be used in an enzyme linked immunosorbent assay (ELISA) for herbicide detection in soil.","abstract_has_math":false,"creators":["Koppatschek, Fritz K."],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Crop Sciences","degree_department":null,"school":null,"contributors":["Liebl, Rex A."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T13:18:15Z","date_published":"2011-05-07T13:18:15Z","updated_at":"2026-07-22T22:25:18Z","subjects":["Agriculture, Agronomy","Chemistry, Agricultural"],"languages":["eng"],"rights":["Copyright 1989 Koppatschek, Fritz K."],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9010922","(UMI)AAI9010922"],"render_values":[{"text":"AAI9010922","href":null,"code":true},{"text":"(UMI)AAI9010922","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/21758","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Liebl, Rex A."]},{"key":"dc:creator","label":"Author","values":["Koppatschek, Fritz K."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T13:18:15Z","10000-01-01","1989"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Crop Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Agriculture, Agronomy","Chemistry, Agricultural"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1989 Koppatschek, Fritz K."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9010922","(UMI)AAI9010922","http://hdl.handle.net/2142/21758"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The purpose of this research was to develop polyclonal antibodies to the soybean herbicides clomazone (2- ((2 chlorophenyl)methyl) -4,4-dimethyl-3-isoxazolidinone), imazaquin (2- (4,5-dihydro-4-methyl-4-(1-methylethyl)-5-oxo-1H-imidazol-2-yl) -3-quinolinecarboxylic acid), and metribuzin (4-amino-6-(1,1-dimethylethyl)-3-(methylthio)-1,2,4-triazin-5(4H)-one). The herbicide-specific antibodies were to be used in an enzyme linked immunosorbent assay (ELISA) for herbicide detection in soil.","Antibodies, specific to clomazone, were produced in New Zealand white rabbits to a BSA-clomazone conjugate. Attempts at producing antibodies specific to imazaquin, via BSA-imazaquin and BSA-hexanoic acid-imazaquin conjugates, were not successful. Antibodies specific to metribuzin, via a porcine gamma globulin-metribuzin conjugate, were not successfully produced.","The antibodies to the BSA-clomazone conjugate were sensitive to clomazone having an I$\\sb{50}$ of 12 ng/mL. The antibodies were also specific to clomazone and demonstrated no cross reactivity to the soybean herbicides metribuzin, metolachlor (2-chloro-N-(2-ethyl-6-methylphenyl)-N-(2-methoxy-1-methylethyl)acetamide) or trifluralin (2,6-dinitro-N,N-dipropyl-4-(trifluromethyl)benzenamine).","Soil extracts, from several different soil types, did not interfere with detection of clomazone in this ELISA. Soil extraction procedures could also be simplified without reducing the sensitivity of the assay.","The ELISA system compared favorably with conventional clomazone detection systems of wheat bioassay and gas chromatography. The low level of detection for both gas chromatography and ELISA was from a 10 ppb acetonitrile extract of clomazone treated soil.","Clomazone-treated soil was extracted with phosphate buffer and clomazone was quantified with ELISA. Detection of clomazone in the phosphate buffer extracts was influenced by soil type, with greater detection in low organic matter soil. This technique offers a novel method for quantifying a portion of the biologically available herbicide.","The ELISA system proved to be a fast, accurate and sensitive method of clomazone detection.","Made available in DSpace on 2011-05-07T13:18:15Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9010922.pdf: 2368570 bytes, checksum: 6c7b0373bc0bd66dfc8022fc3bdcff08 (MD5) Previous issue date: 1989","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:53:00Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:24:28-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Enzyme-linked immunosorbent assay for herbicide detection"]}]}],"canonical_facts":{"dc:contributor":["Liebl, Rex A."],"dc:creator":["Koppatschek, Fritz K."],"dc:date":["2011-05-07T13:18:15Z","10000-01-01","1989"],"dc:description":["The purpose of this research was to develop polyclonal antibodies to the soybean herbicides clomazone (2- ((2 chlorophenyl)methyl) -4,4-dimethyl-3-isoxazolidinone), imazaquin (2- (4,5-dihydro-4-methyl-4-(1-methylethyl)-5-oxo-1H-imidazol-2-yl) -3-quinolinecarboxylic acid), and metribuzin (4-amino-6-(1,1-dimethylethyl)-3-(methylthio)-1,2,4-triazin-5(4H)-one). The herbicide-specific antibodies were to be used in an enzyme linked immunosorbent assay (ELISA) for herbicide detection in soil.","Antibodies, specific to clomazone, were produced in New Zealand white rabbits to a BSA-clomazone conjugate. Attempts at producing antibodies specific to imazaquin, via BSA-imazaquin and BSA-hexanoic acid-imazaquin conjugates, were not successful. Antibodies specific to metribuzin, via a porcine gamma globulin-metribuzin conjugate, were not successfully produced.","The antibodies to the BSA-clomazone conjugate were sensitive to clomazone having an I$\\sb{50}$ of 12 ng/mL. The antibodies were also specific to clomazone and demonstrated no cross reactivity to the soybean herbicides metribuzin, metolachlor (2-chloro-N-(2-ethyl-6-methylphenyl)-N-(2-methoxy-1-methylethyl)acetamide) or trifluralin (2,6-dinitro-N,N-dipropyl-4-(trifluromethyl)benzenamine).","Soil extracts, from several different soil types, did not interfere with detection of clomazone in this ELISA. Soil extraction procedures could also be simplified without reducing the sensitivity of the assay.","The ELISA system compared favorably with conventional clomazone detection systems of wheat bioassay and gas chromatography. The low level of detection for both gas chromatography and ELISA was from a 10 ppb acetonitrile extract of clomazone treated soil.","Clomazone-treated soil was extracted with phosphate buffer and clomazone was quantified with ELISA. Detection of clomazone in the phosphate buffer extracts was influenced by soil type, with greater detection in low organic matter soil. This technique offers a novel method for quantifying a portion of the biologically available herbicide.","The ELISA system proved to be a fast, accurate and sensitive method of clomazone detection.","Made available in DSpace on 2011-05-07T13:18:15Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9010922.pdf: 2368570 bytes, checksum: 6c7b0373bc0bd66dfc8022fc3bdcff08 (MD5) Previous issue date: 1989","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:53:00Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:24:28-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9010922","(UMI)AAI9010922","http://hdl.handle.net/2142/21758"],"dc:language":["eng"],"dc:rights":["Copyright 1989 Koppatschek, Fritz K."],"dc:subject":["Agriculture, Agronomy","Chemistry, Agricultural"],"dc:title":["Enzyme-linked immunosorbent assay for herbicide detection"],"dc:type":["text"],"thesis:degree_discipline":["Crop Sciences"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:18Z"}