{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/21515"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/21515","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Flow cytometric analysis of the maize genome","abstract":"Studies on analysis of the maize genome using flow cytometry were carried out at the University of Illinois, Urbana-Champaign. These studies were performed in order to gain a better understanding of the maize genome, its effect on plant performance and factors which could disrupt it.","abstract_html":"Studies on analysis of the maize genome using flow cytometry were carried out at the University of Illinois, Urbana-Champaign. These studies were performed in order to gain a better understanding of the maize genome, its effect on plant performance and factors which could disrupt it.","abstract_has_math":false,"creators":["Biradar, Digambareppa Pandappa"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Crop Sciences","degree_department":null,"school":null,"contributors":["Bullock, Donald G."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T13:10:52Z","date_published":"2011-05-07T13:10:52Z","updated_at":"2026-07-22T22:25:18Z","subjects":["Agriculture, Agronomy","Biology, Genetics"],"languages":["eng"],"rights":["Copyright 1993 Biradar, Digambareppa Pandappa"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9411568","(UMI)AAI9411568"],"render_values":[{"text":"AAI9411568","href":null,"code":true},{"text":"(UMI)AAI9411568","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/21515","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Bullock, Donald G."]},{"key":"dc:creator","label":"Author","values":["Biradar, Digambareppa Pandappa"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T13:10:52Z","10000-01-01","1993"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Crop Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Agriculture, Agronomy","Biology, Genetics"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1993 Biradar, Digambareppa Pandappa"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9411568","(UMI)AAI9411568","http://hdl.handle.net/2142/21515"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Studies on analysis of the maize genome using flow cytometry were carried out at the University of Illinois, Urbana-Champaign. These studies were performed in order to gain a better understanding of the maize genome, its effect on plant performance and factors which could disrupt it.","In the first two studies, the nuclear DNA content of several F1 hybrids was analyzed using the fluorochrome DAPI (4$\\sp\\prime$-6-diamidino-2-phenylindole). Five hybrids were observed to have had significantly higher nuclear DNA content than their respective parental means. In hybrids that had a low heterotic response, the observed nuclear DNA content exceeded the expected DNA amount. The unstable inheritance of DNA in these hybrids suggests a relationship between heterosis (an agronomic trait) and nuclear DNA content.","In the third, fourth and fifth studies, intraplant variation in nuclear DNA content was investigated. Significant differences were observed with respect to nuclei distribution in the higher ploidy peaks of diploid and tetraploid maize tissues. Variation in the degree of endopolyploidy observed in maize appeared to have an effect on overall agronomic performance under field conditions. Intraplant variations with respect to DNA content in maize diploid nuclei were observed as well. Nuclei from meristematic root and stem had similar amounts of DNA. Nuclei from differentiated mesocotyl and nodal root had lower amounts of DNA. Propidium iodide (PI) in conjunction with DAPI was used for probing chromatin structure in maize diploid nuclei isolated from meristematic and differentiating tissues. Nuclei from various tissues were found to have different chromatin structure.","In the sixth study, nuclear alterations were observed in seedlings grown from kernels treated with benzimidazole RP-727 fungicide. The magnitude of alterations were minute. The alterations were found to be different in various plant organs analyzed. The results indicated that agrichemicals can potentially alter the genome organization of plant nuclei.","In the last study, agronomically important growth and yield parameters showed negative correlations with nuclear DNA amount in maize. These correlations demonstrate how the nucleotype may exhibit a high degree of influence on the agronomic phenotype.","Made available in DSpace on 2011-05-07T13:10:52Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9411568.pdf: 4797256 bytes, checksum: 28646813d6b27497fd097188af99f2b6 (MD5) Previous issue date: 1993","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:51:19Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:23:32-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Flow cytometric analysis of the maize genome"]}]}],"canonical_facts":{"dc:contributor":["Bullock, Donald G."],"dc:creator":["Biradar, Digambareppa Pandappa"],"dc:date":["2011-05-07T13:10:52Z","10000-01-01","1993"],"dc:description":["Studies on analysis of the maize genome using flow cytometry were carried out at the University of Illinois, Urbana-Champaign. These studies were performed in order to gain a better understanding of the maize genome, its effect on plant performance and factors which could disrupt it.","In the first two studies, the nuclear DNA content of several F1 hybrids was analyzed using the fluorochrome DAPI (4$\\sp\\prime$-6-diamidino-2-phenylindole). Five hybrids were observed to have had significantly higher nuclear DNA content than their respective parental means. In hybrids that had a low heterotic response, the observed nuclear DNA content exceeded the expected DNA amount. The unstable inheritance of DNA in these hybrids suggests a relationship between heterosis (an agronomic trait) and nuclear DNA content.","In the third, fourth and fifth studies, intraplant variation in nuclear DNA content was investigated. Significant differences were observed with respect to nuclei distribution in the higher ploidy peaks of diploid and tetraploid maize tissues. Variation in the degree of endopolyploidy observed in maize appeared to have an effect on overall agronomic performance under field conditions. Intraplant variations with respect to DNA content in maize diploid nuclei were observed as well. Nuclei from meristematic root and stem had similar amounts of DNA. Nuclei from differentiated mesocotyl and nodal root had lower amounts of DNA. Propidium iodide (PI) in conjunction with DAPI was used for probing chromatin structure in maize diploid nuclei isolated from meristematic and differentiating tissues. Nuclei from various tissues were found to have different chromatin structure.","In the sixth study, nuclear alterations were observed in seedlings grown from kernels treated with benzimidazole RP-727 fungicide. The magnitude of alterations were minute. The alterations were found to be different in various plant organs analyzed. The results indicated that agrichemicals can potentially alter the genome organization of plant nuclei.","In the last study, agronomically important growth and yield parameters showed negative correlations with nuclear DNA amount in maize. These correlations demonstrate how the nucleotype may exhibit a high degree of influence on the agronomic phenotype.","Made available in DSpace on 2011-05-07T13:10:52Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9411568.pdf: 4797256 bytes, checksum: 28646813d6b27497fd097188af99f2b6 (MD5) Previous issue date: 1993","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:51:19Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:23:32-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9411568","(UMI)AAI9411568","http://hdl.handle.net/2142/21515"],"dc:language":["eng"],"dc:rights":["Copyright 1993 Biradar, Digambareppa Pandappa"],"dc:subject":["Agriculture, Agronomy","Biology, Genetics"],"dc:title":["Flow cytometric analysis of the maize genome"],"dc:type":["text"],"thesis:degree_discipline":["Crop Sciences"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:18Z"}