{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/21375"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/21375","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Characteristics of sodium transport through the amiloride-sensitive sodium-magnesium pathway in hamster red cells","abstract":"In hamster red cells, amiloride-sensitive Na influx, 0.8 mmol/l cell/hr, is the only, carrier-mediated, Mg-dependent pathway for inward Na transport. This Na-dependent Mg transport is involved in the regulation of intracellular Mg$\\sp{2+}$ concentration under physiological conditions.","abstract_html":"In hamster red cells, amiloride-sensitive Na influx, 0.8 mmol/l cell/hr, is the only, carrier-mediated, Mg-dependent pathway for inward Na transport. This Na-dependent Mg transport is involved in the regulation of intracellular Mg$\\sp{2+}$ concentration under physiological conditions.","abstract_has_math":true,"creators":["Xu, Wan Yan"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Physiology","degree_department":null,"school":null,"contributors":["Willis, John S."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T13:06:51Z","date_published":"2011-05-07T13:06:51Z","updated_at":"2026-07-22T22:25:17Z","subjects":["Biology, Cell","Biology, Animal Physiology","Biology, Zoology"],"languages":["eng"],"rights":["Copyright 1993 Xu, Wan Yan"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9411831","(UMI)AAI9411831"],"render_values":[{"text":"AAI9411831","href":null,"code":true},{"text":"(UMI)AAI9411831","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/21375","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Willis, John S."]},{"key":"dc:creator","label":"Author","values":["Xu, Wan Yan"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T13:06:51Z","10000-01-01","1993"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Physiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Cell","Biology, Animal Physiology","Biology, Zoology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1993 Xu, Wan Yan"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9411831","(UMI)AAI9411831","http://hdl.handle.net/2142/21375"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["In hamster red cells, amiloride-sensitive Na influx, 0.8 mmol/l cell/hr, is the only, carrier-mediated, Mg-dependent pathway for inward Na transport. This Na-dependent Mg transport is involved in the regulation of intracellular Mg$\\sp{2+}$ concentration under physiological conditions.","The Na-Mg transport system was studied in hamster red cells by unidirectional Na fluxes, as well as by net Mg movement. This amiloride-sensitive Na influx can be greatly stimulated by intracellular Mg$\\sp{2+}$. Kinetic studies of both Na influx and Mg efflux in parallel show that a cooperative mechanism is involved in the activation by Mg$\\sb{\\rm i}$ with a Mg$\\sb{0.5}$ of 1.7-1.8 mM and a Hill coefficient of 1.7-1.8 and that activation by Na$\\sb{\\rm o}$ fits a simple Michaelis-Menten equation, with a K$\\sb{\\rm m}$ of 83-104 mM. The similarity of the kinetics for Na and Mg activation and for amiloride inhibition (IC$\\sb{50}$ = 0.3 mM) for both Na influx and net Mg loss confirm that transport of Mg$\\sp{2+}$ is coupled with Na$\\sp+$ with a ratio of 2 Na:1 Mg.","Amiloride-sensitive Na influx is rapidly activated by hypotonic incubation in both fresh and Mg-loaded cells. Amiloride-sensitive Na influx requires ATP. Kinase promoters, phorbol ester and dibutyl cAMP, and phosphatase inhibitors, vanadate and okadaic acid, did not stimulate amiloride-sensitive Na influx.","There was virtually no amiloride-sensitive Na efflux in hamster red cells with high (Na$\\sp+$) $\\sb{\\rm i}$ and (Mg$\\sp{2+}$) $\\sb{\\rm o}$, nor with hypotonicity, nor with ATP depletion.","The ATP-dependence, asymmetry of Na transport, volume dependence and apparent lack of dependence on phosphorylation mechanisms all suggest that the Na-Mg transport mechanism in hamster red cells is not a simple carrier.","Made available in DSpace on 2011-05-07T13:06:51Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9411831.pdf: 5054278 bytes, checksum: 191748814d8c5556e2caca686664c857 (MD5) Previous issue date: 1993","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:50:21Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:22:59-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Characteristics of sodium transport through the amiloride-sensitive sodium-magnesium pathway in hamster red cells"]}]}],"canonical_facts":{"dc:contributor":["Willis, John S."],"dc:creator":["Xu, Wan Yan"],"dc:date":["2011-05-07T13:06:51Z","10000-01-01","1993"],"dc:description":["In hamster red cells, amiloride-sensitive Na influx, 0.8 mmol/l cell/hr, is the only, carrier-mediated, Mg-dependent pathway for inward Na transport. This Na-dependent Mg transport is involved in the regulation of intracellular Mg$\\sp{2+}$ concentration under physiological conditions.","The Na-Mg transport system was studied in hamster red cells by unidirectional Na fluxes, as well as by net Mg movement. This amiloride-sensitive Na influx can be greatly stimulated by intracellular Mg$\\sp{2+}$. Kinetic studies of both Na influx and Mg efflux in parallel show that a cooperative mechanism is involved in the activation by Mg$\\sb{\\rm i}$ with a Mg$\\sb{0.5}$ of 1.7-1.8 mM and a Hill coefficient of 1.7-1.8 and that activation by Na$\\sb{\\rm o}$ fits a simple Michaelis-Menten equation, with a K$\\sb{\\rm m}$ of 83-104 mM. The similarity of the kinetics for Na and Mg activation and for amiloride inhibition (IC$\\sb{50}$ = 0.3 mM) for both Na influx and net Mg loss confirm that transport of Mg$\\sp{2+}$ is coupled with Na$\\sp+$ with a ratio of 2 Na:1 Mg.","Amiloride-sensitive Na influx is rapidly activated by hypotonic incubation in both fresh and Mg-loaded cells. Amiloride-sensitive Na influx requires ATP. Kinase promoters, phorbol ester and dibutyl cAMP, and phosphatase inhibitors, vanadate and okadaic acid, did not stimulate amiloride-sensitive Na influx.","There was virtually no amiloride-sensitive Na efflux in hamster red cells with high (Na$\\sp+$) $\\sb{\\rm i}$ and (Mg$\\sp{2+}$) $\\sb{\\rm o}$, nor with hypotonicity, nor with ATP depletion.","The ATP-dependence, asymmetry of Na transport, volume dependence and apparent lack of dependence on phosphorylation mechanisms all suggest that the Na-Mg transport mechanism in hamster red cells is not a simple carrier.","Made available in DSpace on 2011-05-07T13:06:51Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9411831.pdf: 5054278 bytes, checksum: 191748814d8c5556e2caca686664c857 (MD5) Previous issue date: 1993","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:50:21Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:22:59-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9411831","(UMI)AAI9411831","http://hdl.handle.net/2142/21375"],"dc:language":["eng"],"dc:rights":["Copyright 1993 Xu, Wan Yan"],"dc:subject":["Biology, Cell","Biology, Animal Physiology","Biology, Zoology"],"dc:title":["Characteristics of sodium transport through the amiloride-sensitive sodium-magnesium pathway in hamster red cells"],"dc:type":["text"],"thesis:degree_discipline":["Physiology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:17Z"}